Anti-Nurr1 antibody (ab176184)
Key features and details
- Rabbit polyclonal to Nurr1
- Suitable for: WB, IHC-P
- Reacts with: Mouse
- Isotype: IgG
Overview
-
Product name
Anti-Nurr1 antibody
See all Nurr1 primary antibodies -
Description
Rabbit polyclonal to Nurr1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Synthetic peptide within Human Nurr1 aa 566-591. The exact sequence is proprietary. The selected peptide was post-synthetically modified to achieve highest antigenicity before coupling to carrier protein using hetero bifunctional cross linker.
Sequence:CTQGLQRIFYLKLEDLVPPPAIIDKL
Database link: P43354 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab176184 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500. Detects a band of approximately 75 kDa (predicted molecular weight: 67 kDa).
|
|
IHC-P |
1/50 - 1/100.
|
Notes |
---|
WB
1/500. Detects a band of approximately 75 kDa (predicted molecular weight: 67 kDa). |
IHC-P
1/50 - 1/100. |
Target
-
Function
Transcriptional regulator which is important for the differentiation and maintenance of meso-diencephalic dopaminergic (mdDA) neurons during development. It is crucial for expression of a set of genes such as SLC6A3, SLC18A2, TH and DRD2 which are essential for development of mdDA neurons. -
Tissue specificity
Expressed in a number of cell lines of T-cell, B-cell and fibroblast origin. Strong expression in brain tissue. -
Sequence similarities
Belongs to the nuclear hormone receptor family. NR4 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
Developmental stage
Rapidly and only very transiently expressed after cell activation, during the G0-G1 transition of the cell cycle. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 18227 Mouse
- SwissProt: Q06219 Mouse
- Unigene: 3507 Mouse
-
Alternative names
- HZF 3 antibody
- HZF3 antibody
- Immediate-early response protein NOT antibody
see all
Images
-
Formalin-fixed, paraffin-embedded mouse kidney tissue stained for Nurr1 using ab176184 at 1:50 dilution (IHC Blocking Buffer) in immunohistochemical analysis. Hematoxylin QS (blue) was used to cunterstain.
-
All lanes : Anti-Nurr1 antibody (ab176184) at 1/500 dilution
Lane 1 : Mouse skeletal muscle tissue lysate
Lane 2 : Mouse heart tissue lysate
Lane 3 : Mouse liver tissue lysate
Lane 4 : Mouse kidney tissue lysate
Lane 5 : Mouse lung tissue lysate
Lane 6 : Mouse large intestine tissue lysate
Lane 7 : Mouse small intestine tissue lysate
Lane 8 : Mouse spleen tissue lysate
Lane 9 : Mouse ovary tissue lysate
Lane 10 : Mouse bladder tissue lysate
Lane 11 : Mouse cerebellum tissue lysate
Lane 12 : Mouse spinal cord tissue lysate
Lane 13 : Mouse brain tissue lysate
Predicted band size: 67 kDa -
Formalin-fixed, paraffin-embedded mouse kidney tissue stained for Nurr1 using ab176184 at 1:50 dilution (IHC Blocking Buffer) in immunohistochemical analysis. Hematoxylin QS (blue) was used to cunterstain.
Datasheets and documents
-
Datasheet download
References (2)
ab176184 has been referenced in 2 publications.
- Liu J et al. The Pathology of Morphine-Inhibited Nerve Repair and Morphine-Induced Nerve Damage Is Mediated via Endoplasmic Reticulum Stress. Front Neurosci 15:618190 (2021). PubMed: 33679302
- Hu L et al. Exosomal miR-409-3p secreted from activated mast cells promotes microglial migration, activation and neuroinflammation by targeting Nr4a2 to activate the NF-?B pathway. J Neuroinflammation 18:68 (2021). PubMed: 33750404