Anti-Nurr1 antibody (ab176184)
Key features and details
- Rabbit polyclonal to Nurr1
- Suitable for: WB, IHC-P
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-Nurr1 antibody
See all Nurr1 primary antibodies -
Description
Rabbit polyclonal to Nurr1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P MouseWB Mouse
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Immunogen
Synthetic peptide within Human Nurr1 aa 566-591. The exact sequence is proprietary. The selected peptide was post-synthetically modified to achieve highest antigenicity before coupling to carrier protein using hetero bifunctional cross linker.
Sequence:CTQGLQRIFYLKLEDLVPPPAIIDKL
Database link: P43354 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab176184 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| IHC-P |
Mouse
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| WB |
Mouse
|
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500. Detects a band of approximately 75 kDa (predicted molecular weight: 67 kDa).
|
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| IHC-P |
1/50 - 1/100.
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| Notes |
|---|
|
WB
1/500. Detects a band of approximately 75 kDa (predicted molecular weight: 67 kDa). |
|
IHC-P
1/50 - 1/100. |
Target
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Function
Transcriptional regulator which is important for the differentiation and maintenance of meso-diencephalic dopaminergic (mdDA) neurons during development. It is crucial for expression of a set of genes such as SLC6A3, SLC18A2, TH and DRD2 which are essential for development of mdDA neurons. -
Tissue specificity
Expressed in a number of cell lines of T-cell, B-cell and fibroblast origin. Strong expression in brain tissue. -
Sequence similarities
Belongs to the nuclear hormone receptor family. NR4 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
Developmental stage
Rapidly and only very transiently expressed after cell activation, during the G0-G1 transition of the cell cycle. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 18227 Mouse
- SwissProt: Q06219 Mouse
- Unigene: 3507 Mouse
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Alternative names
- HZF 3 antibody
- HZF3 antibody
- Immediate-early response protein NOT antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nurr1 antibody (ab176184)
Formalin-fixed, paraffin-embedded mouse kidney tissue stained for Nurr1 using ab176184 at 1:50 dilution (IHC Blocking Buffer) in immunohistochemical analysis. Hematoxylin QS (blue) was used to cunterstain.
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Western blot - Anti-Nurr1 antibody (ab176184)All lanes : Anti-Nurr1 antibody (ab176184) at 1/500 dilution
Lane 1 : Mouse skeletal muscle tissue lysate
Lane 2 : Mouse heart tissue lysate
Lane 3 : Mouse liver tissue lysate
Lane 4 : Mouse kidney tissue lysate
Lane 5 : Mouse lung tissue lysate
Lane 6 : Mouse large intestine tissue lysate
Lane 7 : Mouse small intestine tissue lysate
Lane 8 : Mouse spleen tissue lysate
Lane 9 : Mouse ovary tissue lysate
Lane 10 : Mouse bladder tissue lysate
Lane 11 : Mouse cerebellum tissue lysate
Lane 12 : Mouse spinal cord tissue lysate
Lane 13 : Mouse brain tissue lysate
Predicted band size: 67 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nurr1 antibody (ab176184)Formalin-fixed, paraffin-embedded mouse kidney tissue stained for Nurr1 using ab176184 at 1:50 dilution (IHC Blocking Buffer) in immunohistochemical analysis. Hematoxylin QS (blue) was used to cunterstain.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab176184 has not yet been referenced specifically in any publications.