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Neuroscience Neurology process Neurodegenerative disease Parkinson's disease Other
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Anti-Nurr1 antibody (ab227260)

  • Datasheet
  • SDS
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Immunocytochemistry/ Immunofluorescence - Anti-Nurr1 antibody (ab227260)
  • Immunocytochemistry/ Immunofluorescence - Anti-Nurr1 antibody (ab227260)

Key features and details

  • Rabbit polyclonal to Nurr1
  • Suitable for: ICC/IF
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-Nurr1 antibody
    See all Nurr1 primary antibodies
  • Description

    Rabbit polyclonal to Nurr1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Pig, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human Nurr1 (internal sequence). The exact sequence is proprietary.
    Database link: P43354

  • Positive control

    • ICC/IF: SK-N-SH and HeLa cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Parkinson's disease
    • Other
    • Neuroscience
    • Neurology process
    • Neural Signal Transduction
    • Immunology
    • Immune System Diseases
    • Autoimmune
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human Nurr1 protein (His tag) (ab241530)

Applications

Our Abpromise guarantee covers the use of ab227260 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Transcriptional regulator which is important for the differentiation and maintenance of meso-diencephalic dopaminergic (mdDA) neurons during development. It is crucial for expression of a set of genes such as SLC6A3, SLC18A2, TH and DRD2 which are essential for development of mdDA neurons.
  • Tissue specificity

    Expressed in a number of cell lines of T-cell, B-cell and fibroblast origin. Strong expression in brain tissue.
  • Sequence similarities

    Belongs to the nuclear hormone receptor family. NR4 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Developmental stage

    Rapidly and only very transiently expressed after cell activation, during the G0-G1 transition of the cell cycle.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession P43354 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 540245[uid] Cow
    • Entrez Gene: 4929 Human
    • Entrez Gene: 18227 Mouse
    • Entrez Gene: 100157120 Pig
    • Entrez Gene: 54278 Rat
    • Entrez Gene: 697077 Rhesus monkey
    • Omim: 601828 Human
    • SwissProt: Q08E53 Cow
    • SwissProt: P43354 Human
    • SwissProt: Q06219 Mouse
    • SwissProt: Q07917 Rat
    • Unigene: 563344 Human
    • Unigene: 3507 Mouse
    • Unigene: 88129 Rat
    see all
  • Alternative names

    • HZF 3 antibody
    • HZF3 antibody
    • Immediate-early response protein NOT antibody
    • Intermediate early receptor protein antibody
    • NGFI B/nur77 beta type transcription factor homolog antibody
    • NOT antibody
    • Nr4a2 antibody
    • NR4A2_HUMAN antibody
    • nuclear receptor of T cells antibody
    • nuclear receptor related 1 antibody
    • Nuclear receptor subfamily 4 group A member 2 antibody
    • Nur related protein 1 homolog antibody
    • nur related protein-1, human homolog of antibody
    • Nurr 1 antibody
    • Orphan nuclear receptor NR4A2 antibody
    • Orphan nuclear receptor NURR1 antibody
    • RNR 1 antibody
    • RNR1 antibody
    • T cell nuclear receptor NOT antibody
    • T-cell nuclear receptor NOT antibody
    • TINUR antibody
    • Transcriptionally inducible nuclear receptor antibody
    • Transcriptionally inducible nuclear receptor related antibody
    • Transcriptionally inducible nuclear receptor related 1 antibody
    • Transcriptionally-inducible nuclear receptor antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Nurr1 antibody (ab227260)
    Immunocytochemistry/ Immunofluorescence - Anti-Nurr1 antibody (ab227260)

    4% paraformaldehyde-fixed SK-N-SH (human neuroblastoma cell line) cells stained for Nurr1 (green) using ab227260 at 1/500 dilution in ICC/IF. 

    Red: Phalloidin, a cytoskeleton marker, at 1/50 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-Nurr1 antibody (ab227260)
    Immunocytochemistry/ Immunofluorescence - Anti-Nurr1 antibody (ab227260)

    Paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Nurr1 (green) using ab227260 at 1/500 dilution in ICC/IF.

    Alpha-tubulin filaments were labeled with an alhpa-tubulin antibody at 1/2000 dilution (Red).

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab227260? Please let us know so that we can cite the reference in this datasheet.

    ab227260 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Nurr1 antibody

    Inconclusive
    Abreviews
    Abreviews
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Sepia officinalis Cell (Nucleus)
    Permeabilization
    Yes - tritonX
    Specification
    Nucleus
    Fixative
    Paraformaldehyde
    Read More

    Laure BONNAUD-PONTICELLI

    Verified customer

    Submitted Jun 27 2019

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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