Anti-Nurr1 antibody (ab227260)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Nurr1 antibody
See all Nurr1 primary antibodies -
Description
Rabbit polyclonal to Nurr1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Pig, Rhesus monkey
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Immunogen
Recombinant fragment within Human Nurr1 (internal sequence). The exact sequence is proprietary.
Database link: P43354 -
Positive control
- ICC/IF: SK-N-SH and HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab227260 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/100 - 1/1000. |
Target
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Function
Transcriptional regulator which is important for the differentiation and maintenance of meso-diencephalic dopaminergic (mdDA) neurons during development. It is crucial for expression of a set of genes such as SLC6A3, SLC18A2, TH and DRD2 which are essential for development of mdDA neurons. -
Tissue specificity
Expressed in a number of cell lines of T-cell, B-cell and fibroblast origin. Strong expression in brain tissue. -
Sequence similarities
Belongs to the nuclear hormone receptor family. NR4 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
Developmental stage
Rapidly and only very transiently expressed after cell activation, during the G0-G1 transition of the cell cycle. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 540245[uid] Cow
- Entrez Gene: 4929 Human
- Entrez Gene: 18227 Mouse
- Entrez Gene: 100157120 Pig
- Entrez Gene: 54278 Rat
- Entrez Gene: 697077 Rhesus monkey
- Omim: 601828 Human
- SwissProt: Q08E53 Cow
see all -
Alternative names
- HZF 3 antibody
- HZF3 antibody
- Immediate-early response protein NOT antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Nurr1 antibody (ab227260)4% paraformaldehyde-fixed SK-N-SH (human neuroblastoma cell line) cells stained for Nurr1 (green) using ab227260 at 1/500 dilution in ICC/IF.
Red: Phalloidin, a cytoskeleton marker, at 1/50 dilution.
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Immunocytochemistry/ Immunofluorescence - Anti-Nurr1 antibody (ab227260)Paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Nurr1 (green) using ab227260 at 1/500 dilution in ICC/IF.
Alpha-tubulin filaments were labeled with an alhpa-tubulin antibody at 1/2000 dilution (Red).
References
ab227260 has not yet been referenced specifically in any publications.
