Anti-Nurr1 antibody (ab227260)
Key features and details
- Rabbit polyclonal to Nurr1
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Nurr1 antibody
See all Nurr1 primary antibodies -
Description
Rabbit polyclonal to Nurr1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Pig, Rhesus monkey
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Immunogen
Recombinant fragment within Human Nurr1 (internal sequence). The exact sequence is proprietary.
Database link: P43354 -
Positive control
- ICC/IF: SK-N-SH and HeLa cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab227260 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/100 - 1/1000. |
Target
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Function
Transcriptional regulator which is important for the differentiation and maintenance of meso-diencephalic dopaminergic (mdDA) neurons during development. It is crucial for expression of a set of genes such as SLC6A3, SLC18A2, TH and DRD2 which are essential for development of mdDA neurons. -
Tissue specificity
Expressed in a number of cell lines of T-cell, B-cell and fibroblast origin. Strong expression in brain tissue. -
Sequence similarities
Belongs to the nuclear hormone receptor family. NR4 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
Developmental stage
Rapidly and only very transiently expressed after cell activation, during the G0-G1 transition of the cell cycle. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 540245[uid] Cow
- Entrez Gene: 4929 Human
- Entrez Gene: 18227 Mouse
- Entrez Gene: 100157120 Pig
- Entrez Gene: 54278 Rat
- Entrez Gene: 697077 Rhesus monkey
- Omim: 601828 Human
- SwissProt: Q08E53 Cow
see all -
Alternative names
- HZF 3 antibody
- HZF3 antibody
- Immediate-early response protein NOT antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Nurr1 antibody (ab227260)
4% paraformaldehyde-fixed SK-N-SH (human neuroblastoma cell line) cells stained for Nurr1 (green) using ab227260 at 1/500 dilution in ICC/IF.
Red: Phalloidin, a cytoskeleton marker, at 1/50 dilution.
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Immunocytochemistry/ Immunofluorescence - Anti-Nurr1 antibody (ab227260)Paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Nurr1 (green) using ab227260 at 1/500 dilution in ICC/IF.
Alpha-tubulin filaments were labeled with an alhpa-tubulin antibody at 1/2000 dilution (Red).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (0)
ab227260 has not yet been referenced specifically in any publications.
