Recombinant Anti-Nurr1 antibody [EPR24121-38] - BSA and Azide free (Capture) (ab305354)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR24121-38] to Nurr1 - BSA and Azide free (Capture)
- Suitable for: Sandwich ELISA
- Reacts with: Human
Overview
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Product name
Anti-Nurr1 antibody [EPR24121-38] - BSA and Azide free (Capture)
See all Nurr1 primary antibodies -
Description
Rabbit monoclonal [EPR24121-38] to Nurr1 - BSA and Azide free (Capture) -
Host species
Rabbit -
Tested applications
Suitable for: Sandwich ELISAmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab305354 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human Nurr1.
The recommended pair for sandwich ELISA is:
Capture: ab305354, Human Nurr1 Capture Antibody (unconjugated)
Detector: ab30535 , Human Nurr1 Detector Antibody (unconjugated)
The reference range value is 78-5000pg/ml.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.20
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR24121-38 -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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sELISA pair antibody
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab305354 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Sandwich ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918) and Streptavidin Conjugation Kit - Lightning-Link® (ab102921) and Biotinylation Kit / Biotin Conjugation Kit (Fast and Type A) - Lightning-Link® (ab201795).
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Notes |
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Sandwich ELISA
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918) and Streptavidin Conjugation Kit - Lightning-Link® (ab102921) and Biotinylation Kit / Biotin Conjugation Kit (Fast and Type A) - Lightning-Link® (ab201795). |
Target
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Function
Transcriptional regulator which is important for the differentiation and maintenance of meso-diencephalic dopaminergic (mdDA) neurons during development. It is crucial for expression of a set of genes such as SLC6A3, SLC18A2, TH and DRD2 which are essential for development of mdDA neurons. -
Tissue specificity
Expressed in a number of cell lines of T-cell, B-cell and fibroblast origin. Strong expression in brain tissue. -
Sequence similarities
Belongs to the nuclear hormone receptor family. NR4 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
Developmental stage
Rapidly and only very transiently expressed after cell activation, during the G0-G1 transition of the cell cycle. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 4929 Human
- Omim: 601828 Human
- SwissProt: P43354 Human
- Unigene: 563344 Human
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Alternative names
- HZF 3 antibody
- HZF3 antibody
- Immediate-early response protein NOT antibody
see all
Images
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Sandwich ELISA of ab305353 with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab305354 has not yet been referenced specifically in any publications.