Overview

  • Product name
    Anti-Nurr1 antibody [N1404] - ChIP Grade
    See all Nurr1 primary antibodies
  • Description
    Mouse monoclonal [N1404] to Nurr1 - ChIP Grade
  • Host species
    Mouse
  • Specificity
    Antibody does not recognize alpha and gamma forms.
  • Tested applications
    Suitable for: WB, ChIP, ELISA, IP, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment: CVQAQYGSSP QGASPASQSY SYHSSGEYSS DFLTPEFVKF SMDLTNTEIT ATTSLPSFST FMDNYSTGYD VKPPCLYQMP LSGQQSSIKV EDIQMHN, corresponding to amino acids 2-99 of Human Nurr1

  • Positive control
    • Rat cerebral nerve cell.

Properties

Applications

Our Abpromise guarantee covers the use of ab41917 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 66 kDa. Non reducing WB 3 µg/ml.
ChIP 1/100.
ELISA Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
IHC-P Use a concentration of 10 µg/ml.
ICC/IF Use at an assay dependent concentration. PubMed: 22800564

Target

  • Function
    Transcriptional regulator which is important for the differentiation and maintenance of meso-diencephalic dopaminergic (mdDA) neurons during development. It is crucial for expression of a set of genes such as SLC6A3, SLC18A2, TH and DRD2 which are essential for development of mdDA neurons.
  • Tissue specificity
    Expressed in a number of cell lines of T-cell, B-cell and fibroblast origin. Strong expression in brain tissue.
  • Sequence similarities
    Belongs to the nuclear hormone receptor family. NR4 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Developmental stage
    Rapidly and only very transiently expressed after cell activation, during the G0-G1 transition of the cell cycle.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • HZF 3 antibody
    • HZF3 antibody
    • Immediate-early response protein NOT antibody
    • Intermediate early receptor protein antibody
    • NGFI B/nur77 beta type transcription factor homolog antibody
    • NOT antibody
    • Nr4a2 antibody
    • NR4A2_HUMAN antibody
    • nuclear receptor of T cells antibody
    • nuclear receptor related 1 antibody
    • Nuclear receptor subfamily 4 group A member 2 antibody
    • Nur related protein 1 homolog antibody
    • nur related protein-1, human homolog of antibody
    • Nurr 1 antibody
    • Orphan nuclear receptor NR4A2 antibody
    • Orphan nuclear receptor NURR1 antibody
    • RNR 1 antibody
    • RNR1 antibody
    • T cell nuclear receptor NOT antibody
    • T-cell nuclear receptor NOT antibody
    • TINUR antibody
    • Transcriptionally inducible nuclear receptor antibody
    • Transcriptionally inducible nuclear receptor related antibody
    • Transcriptionally inducible nuclear receptor related 1 antibody
    • Transcriptionally-inducible nuclear receptor antibody
    see all

Images

  • ChIP analysis using ab41917 binding Nurr1 in Mouse microglia whole cell lysate. Cells were cross-linked for 10 minutes with 1% formaldehyde. Samples were incubated with primary antibody (1/100 in ChIP dilution buffer - 0.01% SDS, 1.1% Triton X-100, 1.2mM EDTA, 16.7mM Tris-HCl (pH8.1), 167mM NaCl, proteanase inhibitor cocktail) for 16 hours at 4°C. Protein binding was detected using real-time PCR.
    Negative Control: BV2 Mouse microglia cells.

    Positive control: LPS treated BV2 Mouse microglia cells.

     

    See Abreview

  • ab41917 staining Nurr1 rat cerebral nerve cell paraffin section (10 ug/mL) by immunohistochemistry, formalin-fixed paraffin embedded sections.

References

This product has been referenced in:
  • Ho ATV  et al. Prostaglandin E2 is essential for efficacious skeletal muscle stem-cell function, augmenting regeneration and strength. Proc Natl Acad Sci U S A 114:6675-6684 (2017). Flow Cyt . Read more (PubMed: 28607093) »
  • Parra-Damas A  et al. CRTC1 mediates preferential transcription at neuronal activity-regulated CRE/TATA promoters. Sci Rep 7:18004 (2017). Read more (PubMed: 29269871) »
See all 7 Publications for this product

Customer reviews and Q&As

1-10 of 12 Abreviews or Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Brain
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1%
Fixative
Formaldehyde

Mr. Carl Hobbs

Verified customer

Submitted Jan 04 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Brain
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative
Formaldehyde

Mr. Carl Hobbs

Verified customer

Submitted Jan 04 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
ChIP
Detection step
Real-time PCR
Sample
Mouse Cell lysate - whole cell (mouse microglia cells)
Specification
mouse microglia cells
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% formaldehyde

Abcam user community

Verified customer

Submitted Sep 09 2013

Question

Product code: 41917
Lot number: GR16596-11
Inquiry: Hello, We ordered Nurr1 antibody (Anti-Nurr1 antibody [N1404] (ab41917), Lot GR16596-11 and we have tried it in different conditions with no positive result. These were the conditions: IHC-paraffin PFA fixed samples, ethanol grade dehydration, rotihistol, paraffin and embedding. 10 μM sections Deparaffination in rotihistol followed by ethanol grades All washes in PBS Antigen retrieval with citric acid and incubation in microwave. Endogenous peroxidases inactivation by incubating samples in 3% H2O2 for 30 min. Blocking: 10% normal goat serum, 0,1 % triton, 1% BSA in PBS or 0,1 % triton, 1% BSA in PBS, or 1 % triton, 1% BSA in PBS 1-2 hours Primary antibody: 10% normal goat serum, 0,1 % triton, 1% BSA in PBS or 0,1 % triton, 1% BSA in PBS overnight 4°C 1/100 or 1/400 Secondary antibody: Biotinilated goat anti-mouse secondary antibody for 2 hours room temperature 1/600 (standardized for other stainings) Amplification: VECTASTAIN® ABC Kit Detection with DAB Peroxidase Substrate Kit, 3,3’-diaminobenzidine (Vector laboratoires) Immunofluorescence in cryosections: 4% PFA fixed samples, 30% sucrose in PBS overnight, then embedding in OCT. 10 μM sections All washes in PBS Blocking: 10% normal goat serum, 0,1 % triton, 1% BSA in PBS or 0,1 % triton, 1% BSA in PBS 1-2 hours Primary antibody: 10% normal goat serum, 0,1 % triton, 1% BSA in PBS or 0,1 % triton, 1% BSA in PBS overnight 4°C 1/100 or 1/400 Secondary antibody: Goat anti-mouse secondary antibody labelled with Cy3 or Cy2 for 2 hours room temperature 1:200 In both situations we obtained non specific staining, specially in blood vessels. Specifical signal was not found. We used midbrain tissue for which signal should be present in dopaminergic neurons. Stainings were performed at the same with other antibodies for which we obtained specific signal.

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Answer

Thank you for your email. I am sorry to hear that you have been experiencing problems with this antibody.
I have read the details you have kindly provided and have following further questions for better understanding of the problem;
- For how long the sections were heated in microwave?
- Could you provide the image?
- Have you done any sort of troubleshooting?
- Which other antibody has been used? Is it against Nurr1 or other proteins?
- What is the purchase order number?
The protein Nurr1 has been shown to be observed in T-cells as well as B cells. http://www.uniprot.org/uniprot/P43354. Could you please explain why it should not stain blood vessels.
Thank you very much for your cooperation. I will look forward to hearing from you soon.

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (macrophage)
Loading amount
200 µg
Specification
macrophage
Treatment
20uM compound X and IP perfomred
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 26°C

Abcam user community

Verified customer

Submitted Sep 13 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Mouse Cell lysate - whole cell (RAW cell (mouse macrophage))
Total protein in input
200 µg
Specification
RAW cell (mouse macrophage)
Immuno-precipitation step
Protein A/G

Abcam user community

Verified customer

Submitted Sep 07 2012

Answer

DISCOUNT CODE: 100%ABR-0000
Expiration date: 06. November 2012

I am very pleased to hear you would like to accept our offer and test ab41917in CHIP.

This code will give you the value ofab41917 off a future order before the expiration date.

https://www.abcam.com/collaborationdiscount

To redeem this offer, please submit an Abreview for CHIP and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site:

https://www.abcam.com/Abreviews.


Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways:
1) Call to place your order and mention the code to our customer service department;
2) Include the code in your fax order;
3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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Answer

Thank you for confirming those details.

I have created the credit notes for those orders as follows:

xxxxxxx


The credit note may be used in one of the following ways:

(1) Redeemed against the original invoice if this hasn't already been paid.
(2) Held on the account for use against a future order.
(3) A full refund can be offered where no other invoices are outstanding.

Please contact your Finance department to confirm how you would like the credit note to be used and ensure it is not redeemed without your knowledge.

To specifically receive a refund please ask your Finance department to contact our Finance department at creditcontrol@abcam.com or by telephone using the information at the “Contact Us” link in the top right corner of our website.

The credit note ID is for your reference only, please refer to the credit note ID in any correspondence with our accounting department. We will send you the completed credit note by email or postal mail with the actual credit note number which will start with the letters CGB.

The reason I asked about whether you had been stripping the membrane is that the bands detected withall threeantibodies is remarkably similar and at the approximate size which you would expect cross reactivity with an endogenous antibody to be detected (heavy chain ˜50 kDa). This is especially true of the blots presented for ab54366 and ab41918. Both of which I assume were used with the same secondary antibody. This could very easily be explored by performing a "no primary" control. Oris this what has alreadybeen performed in the blot on the top right handside?

I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service should you require furtherassistance.

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Answer

Thank you for contacting us and confirming those details.

Kate is currently away from the office but I will try to help resolve this case as best I can. In order to arrange the credit note to be issued for the three products (ab41917, ab41918 and ab45366) I will need the order numbers. I currently have one order number (1054582 , purchase order number VK3 / 4500392492) for ab41917. Could you please give me the order details of ab41918 and ab45366. If you don't have the order number then the approximate date of delivery? Once I have this information I will issue the credit notes for you.

Having reviewed your correspondence with Kate, would you mind if I ask another question? The three blots you shared with us, were they obtained from individual membranes? Or were the membranes probed for one target then stripped and re-probed? If they were stripped, how was this process performed? Do you think it would be possible that the second probe is picking up the secondary antibody used in the first blot?

I look forward to receiving your reply.

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Question
Answer

Thank you for your message.

I appreciate the experience of your team and the time you have spent on these experiments in the laboratory. I fully understand your concerns and it is disappointing the results have not been successful.

I would like to reassure you that these antibodies (ab41917, ab41918 and ab45366) are tested and covered by our 6 month guarantee in WB and human samples. We are happy to offer a refund, credit note or free of charge replacement when a product is not working in a successfully tested applications or species within the guarantee period. However, we do often find that suggesting some scientifically thought out optimization tips helps to improve the results. So I hope you can understand that we like to offer the best technical support possible to provide a satisfactory outcome. It is also helpful for us to obtain as much information as possible for our quality monitoring investigations.

On this occasion, as you state you have already spent much time optimizing the procedure with no success, I would be pleased to offer an immediate free of charge replacement or credit note for these three items.

With regards to product quality, I would like to reassure you that we monitor feedback closely on a weekly basis and we are not currently concerned about the general quality of these antibodies. Regrettably, I can suggest you have receivedsome bad vials on this occasion. However, as with all our customer correspondence, this enquiry will be kept on our records to be included with ongoing monitoring. With regards to the question of the incorrect band size, if the experiments have been optimized I can only suggest that the antibodies are unfortunately binding non specifically for some reason in this case. I am not able to find anything in the literature to indicate if these proteins may be detected at this size.

Thank you for your understanding and your continued cooperation. I look forward to hearing from you with details of how you would like to proceed.

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1-10 of 12 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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