Our Abpromise guarantee covers the use of ab41917 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 66 kDa. Non reducing WB 3 µg/ml.|
|ELISA||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
|IHC-P||Use a concentration of 10 µg/ml.|
|ICC/IF||Use at an assay dependent concentration. PubMed: 22800564|
ChIP analysis using ab41917 binding Nurr1 in Mouse microglia whole cell lysate. Cells were cross-linked for 10 minutes with 1% formaldehyde. Samples were incubated with primary antibody (1/100 in ChIP dilution buffer - 0.01% SDS, 1.1% Triton X-100, 1.2mM EDTA, 16.7mM Tris-HCl (pH8.1), 167mM NaCl, proteanase inhibitor cocktail) for 16 hours at 4°C. Protein binding was detected using real-time PCR.
Negative Control: BV2 Mouse microglia cells.
Positive control: LPS treated BV2 Mouse microglia cells.