Overview

  • Product name

  • Description

    Rabbit polyclonal to NUSAP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human NUSAP (C terminal).

  • Positive control

    • HT29 cell lysate.
  • General notes

    Previously labelled as NUSAP1.

Properties

Applications

Our Abpromise guarantee covers the use of ab137230 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 49 kDa.

Target

  • Function

    Microtubule-associated protein with the capacity to bundle and stabilize microtubules (By similarity). May associate with chromosomes and promote the organization of mitotic spindle microtubules around them.
  • Sequence similarities

    Belongs to the NUSAP family.
  • Domain

    The KEN box is required for the FZR1-dependent degradation of this protein subsequent to ubiquitination.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
    Ubiquitinated. Ubiquitination by FZR1 may lead to proteasome-dependent degradation of this protein.
  • Cellular localization

    Cytoplasm. Nucleus > nucleolus. Cytoplasm > cytoskeleton > spindle. Chromosome. Found in the cytoplasm and nucleolus during interphase and redistributes to the mitotic spindle in prometaphase (By similarity). Localizes to the mitotic spindle during anaphase and telophase then disappears from around the chromosomes during cytokinesis (By similarity). Localizes to multiple distinct regions of chromosomes throughout mitosis.
  • Information by UniProt
  • Database links

  • Alternative names

    • 2610201A12Rik antibody
    • AI481307 antibody
    • ANKT antibody
    • AW547774 antibody
    • BB165529 antibody
    • BM037 antibody
    • FLJ13421 antibody
    • LNP antibody
    • Nucleolar and spindle associated protein 1 antibody
    • Nucleolar and spindle-associated protein 1 antibody
    • Nucleolar protein ANKT antibody
    • NuSAP antibody
    • NUSAP_HUMAN antibody
    • nusap1 antibody
    • PRO0310p1 antibody
    • Q0310 antibody
    • RP23-22A15.4 antibody
    • SAPL antibody
    see all

Images

  • Anti-NUSAP antibody (ab137230) at 1/500 dilution + HT29 cell lysate at 30 µg

    Predicted band size: 49 kDa

References

ab137230 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Sample
Human Cell lysate - whole cell (HAP1, U2OS, HeLa, HEK293)
Gel Running Conditions
Reduced Denaturing
Loading amount
40 µg
Specification
HAP1, U2OS, HeLa, HEK293
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jan 05 2017

Application
Western blot
Sample
Mouse Tissue lysate - whole (MEF and mouse brain lysates)
Gel Running Conditions
Reduced Denaturing (4-12 bis tris)
Loading amount
40 µg
Specification
MEF and mouse brain lysates
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Nov 08 2016

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