Anti-NUSAP antibody (ab137230)
Key features and details
- Rabbit polyclonal to NUSAP
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-NUSAP antibody
See all NUSAP primary antibodies -
Description
Rabbit polyclonal to NUSAP -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human NUSAP (C terminal).
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Positive control
- HT29 cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
PBS is without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137230 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (2) |
1/500 - 1/1000. Predicted molecular weight: 49 kDa.
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Notes |
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WB
1/500 - 1/1000. Predicted molecular weight: 49 kDa. |
Target
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Function
Microtubule-associated protein with the capacity to bundle and stabilize microtubules (By similarity). May associate with chromosomes and promote the organization of mitotic spindle microtubules around them. -
Sequence similarities
Belongs to the NUSAP family. -
Domain
The KEN box is required for the FZR1-dependent degradation of this protein subsequent to ubiquitination. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Ubiquitinated. Ubiquitination by FZR1 may lead to proteasome-dependent degradation of this protein. -
Cellular localization
Cytoplasm. Nucleus > nucleolus. Cytoplasm > cytoskeleton > spindle. Chromosome. Found in the cytoplasm and nucleolus during interphase and redistributes to the mitotic spindle in prometaphase (By similarity). Localizes to the mitotic spindle during anaphase and telophase then disappears from around the chromosomes during cytokinesis (By similarity). Localizes to multiple distinct regions of chromosomes throughout mitosis. - Information by UniProt
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Database links
- Entrez Gene: 51203 Human
- Omim: 612818 Human
- SwissProt: Q9BXS6 Human
- Unigene: 615092 Human
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Alternative names
- 2610201A12Rik antibody
- AI481307 antibody
- ANKT antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab137230 has been referenced in 3 publications.
- Hou J et al. Increased NUSAP1 expression is associated with lymph node metastasis and survival prognosis in bladder urothelial carcinoma. Sci Rep 12:7003 (2022). PubMed: 35487972
- Sun L et al. Overexpression of NuSAP1 is predictive of an unfavourable prognosis and promotes proliferation and invasion of triple-negative breast cancer cells via the Wnt/ß-catenin/EMT signalling axis. Gene 747:144657 (2020). PubMed: 32298762
- Gao S et al. Nucleolar and Spindle Associated Protein 1 (NUSAP1) Promotes Bladder Cancer Progression Through the TGF-ß Signaling Pathway. Onco Targets Ther 13:813-825 (2020). PubMed: 32099387