Key features and details
- Mouse polyclonal to NUSAP
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-NUSAP antibody
See all NUSAP primary antibodies
DescriptionMouse polyclonal to NUSAP
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human NUSAP aa 216-441.
MNELKQQPINKGGVRTPVPPRGRLSVASTPISQRRSQGRSCGPASQSTLG LKGSLKRSAISAAKTGVRFSAATKDNEHKRSLTKTPARKSAHVTVSGGTP KGEAVLGTHKLKTITGNSAAVITPFKLTTEATQTPVSNKKPVFDLKASLS RPLNYEPHKGKLKPWGQSKENNYLNQHVNRINFYKKTYKQPHLQTKEEQR KKREQERKEKKAKVLGMRRGLILAED
Database link: NP_057443
- HeLa cells. NuSAP-transfected 293T cell lysate.
Previously labelled as NUSAP1.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferpH: 7.4
Constituent: 99% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab169083 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 49 kDa.|
|ICC/IF||Use a concentration of 10 µg/ml.|
FunctionMicrotubule-associated protein with the capacity to bundle and stabilize microtubules (By similarity). May associate with chromosomes and promote the organization of mitotic spindle microtubules around them.
Sequence similaritiesBelongs to the NUSAP family.
DomainThe KEN box is required for the FZR1-dependent degradation of this protein subsequent to ubiquitination.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Ubiquitinated. Ubiquitination by FZR1 may lead to proteasome-dependent degradation of this protein.
Cellular localizationCytoplasm. Nucleus > nucleolus. Cytoplasm > cytoskeleton > spindle. Chromosome. Found in the cytoplasm and nucleolus during interphase and redistributes to the mitotic spindle in prometaphase (By similarity). Localizes to the mitotic spindle during anaphase and telophase then disappears from around the chromosomes during cytokinesis (By similarity). Localizes to multiple distinct regions of chromosomes throughout mitosis.
- Information by UniProt
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All lanes : Anti-NUSAP antibody (ab169083) at 1 µg/ml
Lane 1 : NUSAP1-transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 15 µl per lane.
All lanes : Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 49 kDa
Immunofluorescent analysis of HeLa cells labeling NuSAP with ab169083 at 10µg/ml.
ab169083 has been referenced in 1 publication.
- Hao L et al. Knockdown of P3H4 inhibits proliferation and invasion of bladder cancer. Aging (Albany NY) 12:2156-2168 (2020). PubMed: 32018225