Key features and details
- Rabbit polyclonal to NUT
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-NUT antibody
See all NUT primary antibodies
DescriptionRabbit polyclonal to NUT
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human NUT aa 900-1000 conjugated to keyhole limpet haemocyanin.
Database link: Q86Y26
- IHC-P: FFPE human testis tissue sections.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab157338 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
Tissue specificitySpecifically expressed in testis.
Involvement in diseaseNote=A chromosomal aberration involving NUT is found in a rare, aggressive, and lethal carcinoma arising in midline organs of young people. Translocation t(15;19)(q14;p13) with BRD4 which produces a BRD4-NUT fusion protein.
Note=A chromosomal aberration involving NUT is found in a rare, aggressive, and lethal carcinoma arising in midline organs of young people. Translocation t(15;9)(q14;q34) with BRD3 which produces a BRD3-NUT fusion protein.
Sequence similaritiesBelongs to the FAM22 family.
modificationsPhosphorylation on Ser-1026, Ser-1029 or Ser-1031 is important for cytoplasmic export.
Cellular localizationCytoplasm. Nucleus. Shuttles between nucleus and cytoplasm.
- Information by UniProt
- C15orf55 antibody
- Chromosome 15 open reading frame 55 antibody
- DKFZp434O192 antibody
IHC image of NUT staining in human testis formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab157338, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
ab157338 has not yet been referenced specifically in any publications.