Product nameAnti-NXF1 antibody [53H8]
See all NXF1 primary antibodies
DescriptionMouse monoclonal [53H8] to NXF1
Tested applicationsSuitable for: Dot blot, ICC/IF, WB, IP, ELISAmore details
Species reactivityReacts with: Human
TAP (Tip Associated Protein)
- HeLa nuclear cell extract
Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab129160 as a replacement.
Storage instructionsShipped at 4°C. Store at -20ºC.
Storage bufferpH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS
Concentration information loading...
Purification notesPurified Immunoglobulin
Our Abpromise guarantee covers the use of ab50609 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Dot blot||Use a concentration of 0.5 - 1 µg/ml.|
|ICC/IF||Use at an assay dependent dilution.|
|WB||Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 70 kDa.|
|IP||Use at an assay dependent dilution.|
|ELISA||Use at an assay dependent dilution.|
FunctionInvolved in the nuclear export of mRNA species bearing retroviral constitutive transport elements (CTE) and in the export of mRNA from the nucleus to the cytoplasm. The NXF1-NXT1 heterodimer is involved in the export of HSP70 mRNA in conjunction with THOC4 and THOC5.
Tissue specificityExpressed ubiquitously.
Sequence similaritiesBelongs to the NXF family.
Contains 4 LRR (leucine-rich) repeats.
Contains 1 NTF2 domain.
Contains 1 RRM (RNA recognition motif) domain.
Contains 1 TAP-C domain.
DomainThe minimal CTE binding domain consists of an RNP-type RNA binding domain (RBD) and leucine-rich repeats.
The nucleoporin binding domain consists of a NTF2 domain (also called NTF2-like domain) and a TAP-C domain (also called UBA-like domain). It has 2 nucleoporin-FG-repeats binding sites (one in the NTF2 and the other in the TAP-C domain) which contribute to nucleoporin association and act synergistically to export cellular mRNAs.
The NTF2 domain is functional only in the presence of NXT1 and is essential for the export of mRNA from the nucleus.
The TAP-C domain mediates direct interactions with nucleoporin-FG-repeats and is necessary and sufficient for localization of NXF1 to the nuclear rim. The conserved loop 594-NWD-596 of the TAP-C domain has a critical role in the interaction with nucleoporins.
The leucine-rich repeats are essential for the export of mRNA from the nucleus.
The RNA-binding domain is a non-canonical RNP-type domain.
Cellular localizationNucleus > nucleoplasm. Nucleus speckle. Cytoplasm. Localized predominantly in the nucleoplasm and at both the nucleoplasmic and cytoplasmic faces of the nuclear pore complex. Shuttles between the nucleus and the cytoplasm. Travels to the cytoplasm as part of the exon junction complex (EJC) bound to mRNA.
- Information by UniProt
- DKFZp667O0311 antibody
- DmNXF1 antibody
- MEX67 antibody
Anti-NXF1 antibody [53H8] (ab50609) at 0.5 µg/ml + Hela nuclear cell extract at 200 µg/ml
Predicted band size: 70 kDa
Observed band size: 62 kDa why is the actual band size different from the predicted?
ab50609 staining NXF1 in HeLa cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed in paraformaldehyde and permeabilized in 0.1% Triton X-100. The primary antibody was diluted 1/1000 in 3% BSA in PBS and incubated with the sample for 1 hour at 25°C. The secondary antibody was Alexa Fluor® 568-conjugated goat anti-mouse polyclonal, diluted 1/1000. DAPI was used for nuclear counterstain.
This product has been referenced in:
- Hautbergue GM et al. SRSF1-dependent nuclear export inhibition of C9ORF72 repeat transcripts prevents neurodegeneration and associated motor deficits. Nat Commun 8:16063 (2017). Read more (PubMed: 28677678) »
- Viphakone N et al. Luzp4 defines a new mRNA export pathway in cancer cells. Nucleic Acids Res 43:2353-66 (2015). Read more (PubMed: 25662211) »