Overview

  • Product name

  • Description

    Rabbit polyclonal to NXF1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human NXF1 aa 569-619. The exact sequence is proprietary. (NP_006353.2).
    Database link: Q9UBU9

  • Positive control

    • WB: HEK-293T, HeLa, Jurkat and NIH/3T3 cell lysate. IP: NXF1 in HEK-293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab240705 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    Involved in the nuclear export of mRNA species bearing retroviral constitutive transport elements (CTE) and in the export of mRNA from the nucleus to the cytoplasm. The NXF1-NXT1 heterodimer is involved in the export of HSP70 mRNA in conjunction with THOC4 and THOC5.
  • Tissue specificity

    Expressed ubiquitously.
  • Sequence similarities

    Belongs to the NXF family.
    Contains 4 LRR (leucine-rich) repeats.
    Contains 1 NTF2 domain.
    Contains 1 RRM (RNA recognition motif) domain.
    Contains 1 TAP-C domain.
  • Domain

    The minimal CTE binding domain consists of an RNP-type RNA binding domain (RBD) and leucine-rich repeats.
    The nucleoporin binding domain consists of a NTF2 domain (also called NTF2-like domain) and a TAP-C domain (also called UBA-like domain). It has 2 nucleoporin-FG-repeats binding sites (one in the NTF2 and the other in the TAP-C domain) which contribute to nucleoporin association and act synergistically to export cellular mRNAs.
    The NTF2 domain is functional only in the presence of NXT1 and is essential for the export of mRNA from the nucleus.
    The TAP-C domain mediates direct interactions with nucleoporin-FG-repeats and is necessary and sufficient for localization of NXF1 to the nuclear rim. The conserved loop 594-NWD-596 of the TAP-C domain has a critical role in the interaction with nucleoporins.
    The leucine-rich repeats are essential for the export of mRNA from the nucleus.
    The RNA-binding domain is a non-canonical RNP-type domain.
  • Cellular localization

    Nucleus > nucleoplasm. Nucleus speckle. Cytoplasm. Localized predominantly in the nucleoplasm and at both the nucleoplasmic and cytoplasmic faces of the nuclear pore complex. Shuttles between the nucleus and the cytoplasm. Travels to the cytoplasm as part of the exon junction complex (EJC) bound to mRNA.
  • Information by UniProt
  • Database links

  • Alternative names

    • DKFZp667O0311 antibody
    • DmNXF1 antibody
    • MEX67 antibody
    • MEX67, yeast, homolog of antibody
    • Mex67p antibody
    • mRNA export factor TAP antibody
    • Mvb1 antibody
    • Nuclear RNA export factor 1 antibody
    • Nuclear RNA export factor 1 homolog (S. cerevisiae) antibody
    • nxf 1 antibody
    • NXF1 antibody
    • NXF1_HUMAN antibody
    • Protein small bristles antibody
    • Sbr antibody
    • TAP antibody
    • Tip associating protein antibody
    • Tip-associated protein antibody
    • Tip-associating protein antibody
    see all

Images

  • All lanes : Anti-NXF1 antibody (ab240705) at 0.1 µg/ml

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
    Lane 2 : HEK-293T whole cell lysate at 15 µg
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
    Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
    Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg

    Exposure time: 30 seconds
  • NXF1 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab240705 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab240705 at 1 µg/ml.

    Lane 1: ab240705 IP in HEK-293T whole cell lysate.
    Lane 2: Control IgG IP in HEK-293T whole cell lysate.

    Detection: Chemiluminescence with exposure time of 3 minutes.

References

ab240705 has not yet been referenced specifically in any publications.

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