Name: Anti-NXF1 antibody [EPR8009]
Brand: RabMAb
SKU: ab129160
Rating: 5 (2 reviews)

Product name

Anti-NXF1 antibody [EPR8009]
See all NXF1 primary antibodies
Description

Rabbit monoclonal [EPR8009] to NXF1
Host species

Rabbit
Tested applications

Suitable for: WB, IP, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt
Species reactivity

Reacts with: Human
Immunogen

Synthetic peptide within Human NXF1. The exact sequence is proprietary.
Positive control
- HeLa, 293T, K562 and HepG2 cell lysates; Human colon tissue; HepG2 cells.
General notes

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab^® patents.
This product is a recombinant rabbit monoclonal antibody.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR8009
Isotype

IgG
Research areas

Alternative Versions
- Anti-NXF1 antibody [EPR8009] - BSA and Azide free (ab248319)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls

Our Abpromise guarantee covers the use of ab129160 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/1000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).
IP		1/10 - 1/100.
IHC-P		1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF		1/250 - 1/500.

Application notes

Is unsuitable for Flow Cyt.

Function

Involved in the nuclear export of mRNA species bearing retroviral constitutive transport elements (CTE) and in the export of mRNA from the nucleus to the cytoplasm. The NXF1-NXT1 heterodimer is involved in the export of HSP70 mRNA in conjunction with THOC4 and THOC5.
Tissue specificity

Expressed ubiquitously.
Sequence similarities

Belongs to the NXF family.
Contains 4 LRR (leucine-rich) repeats.
Contains 1 NTF2 domain.
Contains 1 RRM (RNA recognition motif) domain.
Contains 1 TAP-C domain.
Domain

The minimal CTE binding domain consists of an RNP-type RNA binding domain (RBD) and leucine-rich repeats.
The nucleoporin binding domain consists of a NTF2 domain (also called NTF2-like domain) and a TAP-C domain (also called UBA-like domain). It has 2 nucleoporin-FG-repeats binding sites (one in the NTF2 and the other in the TAP-C domain) which contribute to nucleoporin association and act synergistically to export cellular mRNAs.
The NTF2 domain is functional only in the presence of NXT1 and is essential for the export of mRNA from the nucleus.
The TAP-C domain mediates direct interactions with nucleoporin-FG-repeats and is necessary and sufficient for localization of NXF1 to the nuclear rim. The conserved loop 594-NWD-596 of the TAP-C domain has a critical role in the interaction with nucleoporins.
The leucine-rich repeats are essential for the export of mRNA from the nucleus.
The RNA-binding domain is a non-canonical RNP-type domain.
Cellular localization

Nucleus > nucleoplasm. Nucleus speckle. Cytoplasm. Localized predominantly in the nucleoplasm and at both the nucleoplasmic and cytoplasmic faces of the nuclear pore complex. Shuttles between the nucleus and the cytoplasm. Travels to the cytoplasm as part of the exon junction complex (EJC) bound to mRNA.
Target information above from: UniProt accession Q9UBU9 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 10482 Human
- Omim: 602647 Human
- SwissProt: Q9UBU9 Human
- Unigene: 523739 Human
- Unigene: 601546 Human
Alternative names
- DKFZp667O0311 antibody
- DmNXF1 antibody
- MEX67 antibody
- MEX67, yeast, homolog of antibody
- Mex67p antibody
- mRNA export factor TAP antibody
- Mvb1 antibody
- Nuclear RNA export factor 1 antibody
- Nuclear RNA export factor 1 homolog (S. cerevisiae) antibody
- nxf 1 antibody
- NXF1 antibody
- NXF1_HUMAN antibody
- Protein small bristles antibody
- Sbr antibody
- TAP antibody
- Tip associating protein antibody
- Tip-associated protein antibody
- Tip-associating protein antibody
see all

Western blot - Anti-NXF1 antibody [EPR8009] (ab129160)

All lanes : Anti-NXF1 antibody [EPR8009] (ab129160) at 1/1000 dilution

Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : K562 cell lysate
Lane 4 : HepG2 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 70 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NXF1 antibody [EPR8009] (ab129160)

ab129160 at 1/100 dilution staining NXF1 in paraffin-embedded Human colon tissue by immunohistochemistry.
Immunocytochemistry/ Immunofluorescence - Anti-NXF1 antibody [EPR8009] (ab129160)

ab129160 at 1/250 dilution staining NXF1 in HepG2 cells by immunofluorescence.
Immunoprecipitation - Anti-NXF1 antibody [EPR8009] (ab129160)

ab129160 at 1/20 immunoprecipitating NXF1 in HepG2 (human hepatocellular carcinoma) whole cell lysate observed at 70 KDa (Lanes 1 and 2).

Lane 1 (input): HepG2 whole cell lysate, 10μg
Lane 2 (+): ab129160 + HepG2 whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab129160 in HepG2 whole cell lysate

For western blotting, ab129160 at 1/500 dilution and ab131366 VeriBlot for IP (HRP) at 1/1000 were used.

Blocking and Diluting buffer and concentration: 5% NFDM/TBST.
Immunoprecipitation - Anti-NXF1 antibody [EPR8009] (ab129160)

ab129160 at 1/20 immunoprecipitating NXF1 in HeLa (human cervix adenocarcinoma) whole cell lysate observed at 70 KDa (Lanes 1 and 2).

Lane 1 (input): HeLa whole cell lysate, 10μg
Lane 2 (+): ab129160 + HeLa whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab129160 in HeLa whole cell lysate

For western blotting, ab129160 at 1/500 dilution and ab131366 VeriBlot for IP (HRP) at 1/1000 were used.

Blocking and Diluting buffer and concentration: 5% NFDM/TBST.

Immunoprecipitation protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Datasheet

This product has been referenced in:

Roundtree IA et al. YTHDC1 mediates nuclear export of N6-methyladenosine methylated mRNAs. Elife 6:N/A (2017). Read more (PubMed: 28984244) »

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Publishing research using ab129160? Please let us know so that we can cite the reference in this datasheet.

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Abcam guarantees this product to work in the species/application used in this Abreview.

Application

Immunoprecipitation

Sample

Human Cell lysate - whole cell (HeLa soluble lysate)

Total protein in input

5e+006 cells

Immuno-precipitation step

Protein A

Specification

HeLa soluble lysate

Abcam user community

Verified customer

Submitted Nov 30 2016

Abcam guarantees this product to work in the species/application used in this Abreview.

Application

Western blot

Sample

Human Cell lysate - whole cell (HeLa)

Gel Running Conditions

Reduced Denaturing (4-12% Bis-Tris)

Loading amount

100 µg

Treatment

siRNA verification

Specification

HeLa

Blocking step

Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Nov 29 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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Anti-NXF1 antibody [EPR8009] (ab129160)

Overview

Properties

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Applications

Target

Images

Protocols

Datasheets and documents

References

This product has been referenced in:

Customer reviews and Q&As

Immunoprecipitation abreview for Anti-NXF1 antibody [EPR8009]

Western blot abreview for Anti-NXF1 antibody [EPR8009]