Overview

  • Product name
    Anti-NXF1 antibody [EPR8009]
    See all NXF1 primary antibodies
  • Description
    Rabbit monoclonal [EPR8009] to NXF1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IP, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human NXF1. The exact sequence is proprietary.

  • Positive control
    • HeLa, 293T, K562 and HepG2 cell lysates; Human colon tissue; HepG2 cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab129160 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/250 - 1/500.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function
      Involved in the nuclear export of mRNA species bearing retroviral constitutive transport elements (CTE) and in the export of mRNA from the nucleus to the cytoplasm. The NXF1-NXT1 heterodimer is involved in the export of HSP70 mRNA in conjunction with THOC4 and THOC5.
    • Tissue specificity
      Expressed ubiquitously.
    • Sequence similarities
      Belongs to the NXF family.
      Contains 4 LRR (leucine-rich) repeats.
      Contains 1 NTF2 domain.
      Contains 1 RRM (RNA recognition motif) domain.
      Contains 1 TAP-C domain.
    • Domain
      The minimal CTE binding domain consists of an RNP-type RNA binding domain (RBD) and leucine-rich repeats.
      The nucleoporin binding domain consists of a NTF2 domain (also called NTF2-like domain) and a TAP-C domain (also called UBA-like domain). It has 2 nucleoporin-FG-repeats binding sites (one in the NTF2 and the other in the TAP-C domain) which contribute to nucleoporin association and act synergistically to export cellular mRNAs.
      The NTF2 domain is functional only in the presence of NXT1 and is essential for the export of mRNA from the nucleus.
      The TAP-C domain mediates direct interactions with nucleoporin-FG-repeats and is necessary and sufficient for localization of NXF1 to the nuclear rim. The conserved loop 594-NWD-596 of the TAP-C domain has a critical role in the interaction with nucleoporins.
      The leucine-rich repeats are essential for the export of mRNA from the nucleus.
      The RNA-binding domain is a non-canonical RNP-type domain.
    • Cellular localization
      Nucleus > nucleoplasm. Nucleus speckle. Cytoplasm. Localized predominantly in the nucleoplasm and at both the nucleoplasmic and cytoplasmic faces of the nuclear pore complex. Shuttles between the nucleus and the cytoplasm. Travels to the cytoplasm as part of the exon junction complex (EJC) bound to mRNA.
    • Information by UniProt
    • Database links
    • Alternative names
      • DKFZp667O0311 antibody
      • DmNXF1 antibody
      • MEX67 antibody
      • MEX67, yeast, homolog of antibody
      • Mex67p antibody
      • mRNA export factor TAP antibody
      • Mvb1 antibody
      • Nuclear RNA export factor 1 antibody
      • Nuclear RNA export factor 1 homolog (S. cerevisiae) antibody
      • nxf 1 antibody
      • NXF1 antibody
      • NXF1_HUMAN antibody
      • Protein small bristles antibody
      • Sbr antibody
      • TAP antibody
      • Tip associating protein antibody
      • Tip-associated protein antibody
      • Tip-associating protein antibody
      see all

    Images

    • All lanes : Anti-NXF1 antibody [EPR8009] (ab129160) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : K562 cell lysate
      Lane 4 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 70 kDa

    • ab129160 at 1/100 dilution staining NXF1 in paraffin-embedded Human colon tissue by immunohistochemistry.
    • ab129160 at 1/250 dilution staining NXF1 in HepG2 cells by immunofluorescence.
    • ab129160 at 1/20 immunoprecipitating NXF1 in HepG2 (human hepatocellular carcinoma) whole cell lysate observed at 70 KDa (Lanes 1 and 2).

      Lane 1 (input): HepG2 whole cell lysate, 10μg
      Lane 2 (+): ab129160 + HepG2 whole cell lysate
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab129160 in HepG2 whole cell lysate

      For western blotting, ab129160 at 1/500 dilution and ab131366 VeriBlot for IP (HRP) at 1/1000 were used.

      Blocking and Diluting buffer and concentration: 5% NFDM/TBST.

    • ab129160 at 1/20 immunoprecipitating NXF1 in HeLa (human cervix adenocarcinoma) whole cell lysate observed at 70 KDa (Lanes 1 and 2).

      Lane 1 (input): HeLa whole cell lysate, 10μg
      Lane 2 (+): ab129160 + HeLa whole cell lysate
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab129160 in HeLa whole cell lysate

      For western blotting, ab129160 at 1/500 dilution and ab131366 VeriBlot for IP (HRP) at 1/1000 were used.

      Blocking and Diluting buffer and concentration: 5% NFDM/TBST.

    References

    This product has been referenced in:
    See 1 Publication for this product

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunoprecipitation
    Sample
    Human Cell lysate - whole cell (HeLa soluble lysate)
    Total protein in input
    5e+006 cells
    Immuno-precipitation step
    Protein A
    Specification
    HeLa soluble lysate

    Abcam user community

    Verified customer

    Submitted Nov 30 2016

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HeLa)
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis-Tris)
    Loading amount
    100 µg
    Treatment
    siRNA verification
    Specification
    HeLa
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C

    Abcam user community

    Verified customer

    Submitted Nov 29 2016

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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