Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8010] to NXF1
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Product nameAnti-NXF1 antibody [EPR8010]
See all NXF1 primary antibodies
DescriptionRabbit monoclonal [EPR8010] to NXF1
Tested applicationsSuitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human NXF1 aa 550-650 (C terminal). The exact sequence is proprietary.
- K562, HeLa and 293T cell lysates
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20ºC.
Dissociation constant (KD)KD = 4.70 x 10 -11 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab129171 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 70 kDa.|
FunctionInvolved in the nuclear export of mRNA species bearing retroviral constitutive transport elements (CTE) and in the export of mRNA from the nucleus to the cytoplasm. The NXF1-NXT1 heterodimer is involved in the export of HSP70 mRNA in conjunction with THOC4 and THOC5.
Tissue specificityExpressed ubiquitously.
Sequence similaritiesBelongs to the NXF family.
Contains 4 LRR (leucine-rich) repeats.
Contains 1 NTF2 domain.
Contains 1 RRM (RNA recognition motif) domain.
Contains 1 TAP-C domain.
DomainThe minimal CTE binding domain consists of an RNP-type RNA binding domain (RBD) and leucine-rich repeats.
The nucleoporin binding domain consists of a NTF2 domain (also called NTF2-like domain) and a TAP-C domain (also called UBA-like domain). It has 2 nucleoporin-FG-repeats binding sites (one in the NTF2 and the other in the TAP-C domain) which contribute to nucleoporin association and act synergistically to export cellular mRNAs.
The NTF2 domain is functional only in the presence of NXT1 and is essential for the export of mRNA from the nucleus.
The TAP-C domain mediates direct interactions with nucleoporin-FG-repeats and is necessary and sufficient for localization of NXF1 to the nuclear rim. The conserved loop 594-NWD-596 of the TAP-C domain has a critical role in the interaction with nucleoporins.
The leucine-rich repeats are essential for the export of mRNA from the nucleus.
The RNA-binding domain is a non-canonical RNP-type domain.
Cellular localizationNucleus > nucleoplasm. Nucleus speckle. Cytoplasm. Localized predominantly in the nucleoplasm and at both the nucleoplasmic and cytoplasmic faces of the nuclear pore complex. Shuttles between the nucleus and the cytoplasm. Travels to the cytoplasm as part of the exon junction complex (EJC) bound to mRNA.
- Information by UniProt
- DKFZp667O0311 antibody
- DmNXF1 antibody
- MEX67 antibody
All lanes : Anti-NXF1 antibody [EPR8010] (ab129171) at 1/1000 dilution
Lane 1 : K562 cell lysates
Lane 2 : HeLa cell lysates
Lane 3 : 293T cell lysates
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution
Predicted band size: 70 kDa
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
ab129171 has not yet been referenced specifically in any publications.