Overview

  • Product name

    Anti-NXF1 antibody [EPR8010]
    See all NXF1 primary antibodies
  • Description

    Rabbit monoclonal [EPR8010] to NXF1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human NXF1 aa 550-650 (C terminal). The exact sequence is proprietary.

  • Positive control

    • K562, HeLa and 293T cell lysates
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab129171 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 70 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • Target

    • Function

      Involved in the nuclear export of mRNA species bearing retroviral constitutive transport elements (CTE) and in the export of mRNA from the nucleus to the cytoplasm. The NXF1-NXT1 heterodimer is involved in the export of HSP70 mRNA in conjunction with THOC4 and THOC5.
    • Tissue specificity

      Expressed ubiquitously.
    • Sequence similarities

      Belongs to the NXF family.
      Contains 4 LRR (leucine-rich) repeats.
      Contains 1 NTF2 domain.
      Contains 1 RRM (RNA recognition motif) domain.
      Contains 1 TAP-C domain.
    • Domain

      The minimal CTE binding domain consists of an RNP-type RNA binding domain (RBD) and leucine-rich repeats.
      The nucleoporin binding domain consists of a NTF2 domain (also called NTF2-like domain) and a TAP-C domain (also called UBA-like domain). It has 2 nucleoporin-FG-repeats binding sites (one in the NTF2 and the other in the TAP-C domain) which contribute to nucleoporin association and act synergistically to export cellular mRNAs.
      The NTF2 domain is functional only in the presence of NXT1 and is essential for the export of mRNA from the nucleus.
      The TAP-C domain mediates direct interactions with nucleoporin-FG-repeats and is necessary and sufficient for localization of NXF1 to the nuclear rim. The conserved loop 594-NWD-596 of the TAP-C domain has a critical role in the interaction with nucleoporins.
      The leucine-rich repeats are essential for the export of mRNA from the nucleus.
      The RNA-binding domain is a non-canonical RNP-type domain.
    • Cellular localization

      Nucleus > nucleoplasm. Nucleus speckle. Cytoplasm. Localized predominantly in the nucleoplasm and at both the nucleoplasmic and cytoplasmic faces of the nuclear pore complex. Shuttles between the nucleus and the cytoplasm. Travels to the cytoplasm as part of the exon junction complex (EJC) bound to mRNA.
    • Information by UniProt
    • Database links

    • Alternative names

      • DKFZp667O0311 antibody
      • DmNXF1 antibody
      • MEX67 antibody
      • MEX67, yeast, homolog of antibody
      • Mex67p antibody
      • mRNA export factor TAP antibody
      • Mvb1 antibody
      • Nuclear RNA export factor 1 antibody
      • Nuclear RNA export factor 1 homolog (S. cerevisiae) antibody
      • nxf 1 antibody
      • NXF1 antibody
      • NXF1_HUMAN antibody
      • Protein small bristles antibody
      • Sbr antibody
      • TAP antibody
      • Tip associating protein antibody
      • Tip-associated protein antibody
      • Tip-associating protein antibody
      see all

    Images

    • All lanes : Anti-NXF1 antibody [EPR8010] (ab129171) at 1/1000 dilution

      Lane 1 : K562 cell lysates
      Lane 2 : HeLa cell lysates
      Lane 3 : 293T cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

      Predicted band size: 70 kDa

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab129171 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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