Product nameAnti-NXF5 antibody
DescriptionRabbit polyclonal to NXF5
Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Rat, Dog
- Jurkat cell lysate; Paraffin-embedded human kidney tissue, Paraffin-embedded human intestine tissue
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab50954 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 2.5 µg/ml. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|ELISA||Use at an assay dependent concentration.
Titre using peptide based assay 1/312500.
|IHC-P||Use a concentration of 4 - 8 µg/ml.|
RelevanceNXF5 is a member of a family of nuclear RNA export factors. Common domain features of this family are a noncanonical RNP-type RNA-binding domain (RBD), 4 leucine-rich repeats (LRRs), a nuclear transport factor 2 (NTF2)-like domain that allows heterodimerization with NTF2-related export protein-1 (NXT1), and a ubiquitin-associated domain that mediates interactions with nucleoporins. The LRRs and NTF2-like domains are required for export activity. Alternative splicing seems to be a common mechanism in this gene family. Five transcript variants that encode different isoforms have been found for the NXF5 gene.
Cellular localizationCytoplasmic and Nuclear. Mainly localized in the cytoplasm particularly in the cell body and neurites of hippocampal neurons. Nuclear localization is also observed. Not detected at nuclear rim.
- Nuclear RNA export factor 5 antibody
- TAP like protein 1 antibody
- TAPL 1 antibody
- TAPL1 antibody
Anti-NXF5 antibody (ab50954) at 2.5 µg/ml + Jurkat cell lysate at 10 µg
Predicted band size: 44 kDa
Observed band size: 44 kDa
Secondary antibody: HRP conjugated anti-Rabbit IgG at 1/50000 to 1/100000 dilution.
Buffer: 5% skim milk in PBS. Gel concentration: 12%.
Tissue: paraffin-embedded human Kidney
Cells with positive label: Epithelial cells of renal tubule (indicated with arrows)
Antibody concentration 4 - 8 µg/ml
Tissue: paraffin-embedded human Intestine
Cells with positive label: Epithelial cells of intestinal villus (indicated with arrows)
Antibody concentration: 4 - 8 µg/ml
ab50954 has not yet been referenced specifically in any publications.