RecombinantRabMAb

Recombinant Anti-NXN/NRX antibody [EPR15177] - BSA and Azide free (ab251080)

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Western blot - Anti-NXN/NRX antibody [EPR15177] - BSA and Azide free (ab251080)
  • Flow Cytometry - Anti-NXN/NRX antibody [EPR15177] - BSA and Azide free (ab251080)
  • Immunocytochemistry - Anti-NXN/NRX antibody [EPR15177] - BSA and Azide free (ab251080)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NXN/NRX antibody [EPR15177] - BSA and Azide free (ab251080)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NXN/NRX antibody [EPR15177] - BSA and Azide free (ab251080)
  • Western blot - Anti-NXN/NRX antibody [EPR15177] - BSA and Azide free (ab251080)
  • Anti-NXN/NRX antibody [EPR15177] - BSA and Azide free (ab251080)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15177] to NXN/NRX - BSA and Azide free
  • Suitable for: Flow Cyt, ICC, WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-NXN/NRX antibody [EPR15177] - BSA and Azide free
    See all NXN/NRX primary antibodies

  • Description

    Rabbit monoclonal [EPR15177] to NXN/NRX - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, ICC, WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human NXN/NRX aa 300-400. The exact sequence is proprietary.
    Database link: Q6DKJ4

  • General notes

    ab251080 is the carrier-free version of ab191429 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab251080 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as NXN

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab251080 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

Images

  • Western blot - Anti-NXN/NRX antibody [EPR15177] - BSA and Azide free (ab251080)
    Western blot - Anti-NXN/NRX antibody [EPR15177] - BSA and Azide free (ab251080)
    All lanes : Anti-NXN/NRX antibody [EPR15177] (ab191429) at 1/10000 dilution

    Lane 1 : 293 lysate
    Lane 2 : HepG2 lysate
    Lane 3 : HeLa lysate
    Lane 4 : SH-SY5Y lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 48 kDa
    Additional bands at: 48 kDa. We are unsure as to the identity of these extra bands.



    This data was developed using ab191429, the same antibody clone in a different buffer formulation.

  • Flow Cytometry - Anti-NXN/NRX antibody [EPR15177] - BSA and Azide free (ab251080)
    Flow Cytometry - Anti-NXN/NRX antibody [EPR15177] - BSA and Azide free (ab251080)
    This data was developed using ab191429, the same antibody clone in a different buffer formulation.Flow Cytometry analysis of NIH/3T3 (mouse embryo) labelling NXN/NRX with purified ab191429 at 1/1000 (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Alexa Fluor® 488 goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.
  • Immunocytochemistry - Anti-NXN/NRX antibody [EPR15177] - BSA and Azide free (ab251080)
    Immunocytochemistry - Anti-NXN/NRX antibody [EPR15177] - BSA and Azide free (ab251080)
    This data was developed using ab191429, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of paraformaldehyde-fixed A431 cells labeling NXN/NRX with ab191429 at 1/250, Goat anti rabbit IgG (Alexa Fluor® 488) at 1/200 and DAPI staining (blue). The two negative controls: 1. Primary ab concentration (anti-NXN/NRX) is 1/100 dilution, Secondary ab (Goat anti mouse IgG (Alexa Fluor®594)) is 1/400 dilution; 2. Primary ab concentration (anti-NXN/NRX) is 1/100 dilution, Secondary ab (Goat anti mouse IgG (Alexa Fluor®594)) is 1/400 dilution.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NXN/NRX antibody [EPR15177] - BSA and Azide free (ab251080)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NXN/NRX antibody [EPR15177] - BSA and Azide free (ab251080)
    This data was developed using ab191429, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded mouse brain tissue labeling NXN/NRX with ab191429 at 1/100 with prediluted HRP Polymer for Rabbit IgG as secondary antibody. Negative control: Using PBS instead of primary antibody. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NXN/NRX antibody [EPR15177] - BSA and Azide free (ab251080)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NXN/NRX antibody [EPR15177] - BSA and Azide free (ab251080)
    This data was developed using ab191429, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human transitional cell carcinoma of bladder tissue labeling NXN/NRX with ab191429 at 1/100 with prediluted HRP Polymer for Rabbit IgG as secondary antibody. Negative control: Using PBS instead of primary antibody. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Western blot - Anti-NXN/NRX antibody [EPR15177] - BSA and Azide free (ab251080)
    Western blot - Anti-NXN/NRX antibody [EPR15177] - BSA and Azide free (ab251080)
    All lanes : Anti-NXN/NRX antibody [EPR15177] (ab191429) at 1/10000 dilution

    Lane 1 : Mouse testis lysate
    Lane 2 : Rat testis lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 48 kDa
    Additional bands at: 48 kDa. We are unsure as to the identity of these extra bands.



    This data was developed using ab191429, the same antibody clone in a different buffer formulation.

     

  • Anti-NXN/NRX antibody [EPR15177] - BSA and Azide free (ab251080)
    Anti-NXN/NRX antibody [EPR15177] - BSA and Azide free (ab251080)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab251080? Please let us know so that we can cite the reference in this datasheet.

ab251080 has not yet been referenced specifically in any publications.

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