For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    nxnnrx-antibody-epr15177-bsa-and-azide-free-ab251080.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Signal Transduction Protein Phosphorylation Ser / Thr Phosphatases
Share by email
RecombinantRabMAb

Recombinant Anti-NXN/NRX antibody [EPR15177] - BSA and Azide free (ab251080)

  • Datasheet
  • Certificate of Compliance
Submit a review Submit a question

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-NXN/NRX antibody [EPR15177] - BSA and Azide free (ab251080)
  • Flow Cytometry - Anti-NXN/NRX antibody [EPR15177] - BSA and Azide free (ab251080)
  • Immunocytochemistry - Anti-NXN/NRX antibody [EPR15177] - BSA and Azide free (ab251080)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NXN/NRX antibody [EPR15177] - BSA and Azide free (ab251080)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NXN/NRX antibody [EPR15177] - BSA and Azide free (ab251080)
  • Western blot - Anti-NXN/NRX antibody [EPR15177] - BSA and Azide free (ab251080)
  • Anti-NXN/NRX antibody [EPR15177] - BSA and Azide free (ab251080)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15177] to NXN/NRX - BSA and Azide free
  • Suitable for: Flow Cyt, ICC, WB, IHC-P
  • Reacts with: Mouse, Rat, Human

You may also be interested in

Conjugation
Product image
PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)

View more associated products

Overview

  • Product name

    Anti-NXN/NRX antibody [EPR15177] - BSA and Azide free
    See all NXN/NRX primary antibodies
  • Description

    Rabbit monoclonal [EPR15177] to NXN/NRX - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, ICC, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human NXN/NRX aa 300-400. The exact sequence is proprietary.
    Database link: Q6DKJ4

  • General notes

    ab251080 is the carrier-free version of ab191429 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab251080 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as NXN

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Clonality

    Monoclonal
  • Clone number

    EPR15177
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Phosphatases
    • Stem Cells
    • Signaling Pathways
    • Wnt
    • Cytoplasmic
    • Stem Cells
    • Signaling Pathways
    • Wnt
    • Nuclear

Associated products

  • Alternative Versions

    • Anti-NXN/NRX antibody [EPR15177] (ab191429)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control - BSA and Azide Free (ab210849)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • Mouse testis normal tissue lysate - total protein (ab29289)
    • HeLa whole cell lysate (ab29545)

Applications

Our Abpromise guarantee covers the use of ab251080 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Functions as a redox-dependent negative regulator of the Wnt signaling pathway. May function as a transcriptional regulator. May regulate phosphatase 2A.
  • Sequence similarities

    Belongs to the nucleoredoxin family.
    Contains 1 thioredoxin domain.
  • Cellular localization

    Cytoplasm > cytosol. Nucleus.
  • Target information above from: UniProt accession Q6DKJ4 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 64359 Human
    • Entrez Gene: 18230 Mouse
    • Entrez Gene: 360577 Rat
    • Omim: 612895 Human
    • SwissProt: Q6DKJ4 Human
    • SwissProt: P97346 Mouse
    • Unigene: 527989 Human
    • Unigene: 27915 Mouse
    • Alternative names

      • FLJ12614 antibody
      • NRX antibody
      • nucleoredoxin 1 antibody
      • Nucleoredoxin antibody
      • Nxn antibody
      • NXN_HUMAN antibody
      • OTTHUMP00000115356 antibody
      • TRG 4 antibody
      see all

    Images

    • Western blot - Anti-NXN/NRX antibody [EPR15177] - BSA and Azide free (ab251080)
      Western blot - Anti-NXN/NRX antibody [EPR15177] - BSA and Azide free (ab251080)
      All lanes : Anti-NXN/NRX antibody [EPR15177] (ab191429) at 1/10000 dilution

      Lane 1 : 293 lysate
      Lane 2 : HepG2 lysate
      Lane 3 : HeLa lysate
      Lane 4 : SH-SY5Y lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 48 kDa
      Additional bands at: 48 kDa. We are unsure as to the identity of these extra bands.



      This data was developed using ab191429, the same antibody clone in a different buffer formulation.

    • Flow Cytometry - Anti-NXN/NRX antibody [EPR15177] - BSA and Azide free (ab251080)
      Flow Cytometry - Anti-NXN/NRX antibody [EPR15177] - BSA and Azide free (ab251080)
      This data was developed using ab191429, the same antibody clone in a different buffer formulation.Flow Cytometry analysis of NIH/3T3 (mouse embryo) labelling NXN/NRX with purified ab191429 at 1/1000 (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Alexa Fluor® 488 goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.
    • Immunocytochemistry - Anti-NXN/NRX antibody [EPR15177] - BSA and Azide free (ab251080)
      Immunocytochemistry - Anti-NXN/NRX antibody [EPR15177] - BSA and Azide free (ab251080)
      This data was developed using ab191429, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of paraformaldehyde-fixed A431 cells labeling NXN/NRX with ab191429 at 1/250, Goat anti rabbit IgG (Alexa Fluor® 488) at 1/200 and DAPI staining (blue). The two negative controls: 1. Primary ab concentration (anti-NXN/NRX) is 1/100 dilution, Secondary ab (Goat anti mouse IgG (Alexa Fluor®594)) is 1/400 dilution; 2. Primary ab concentration (anti-NXN/NRX) is 1/100 dilution, Secondary ab (Goat anti mouse IgG (Alexa Fluor®594)) is 1/400 dilution.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NXN/NRX antibody [EPR15177] - BSA and Azide free (ab251080)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NXN/NRX antibody [EPR15177] - BSA and Azide free (ab251080)
      This data was developed using ab191429, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded mouse brain tissue labeling NXN/NRX with ab191429 at 1/100 with prediluted HRP Polymer for Rabbit IgG as secondary antibody. Negative control: Using PBS instead of primary antibody. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NXN/NRX antibody [EPR15177] - BSA and Azide free (ab251080)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NXN/NRX antibody [EPR15177] - BSA and Azide free (ab251080)
      This data was developed using ab191429, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human transitional cell carcinoma of bladder tissue labeling NXN/NRX with ab191429 at 1/100 with prediluted HRP Polymer for Rabbit IgG as secondary antibody. Negative control: Using PBS instead of primary antibody. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
    • Western blot - Anti-NXN/NRX antibody [EPR15177] - BSA and Azide free (ab251080)
      Western blot - Anti-NXN/NRX antibody [EPR15177] - BSA and Azide free (ab251080)
      All lanes : Anti-NXN/NRX antibody [EPR15177] (ab191429) at 1/10000 dilution

      Lane 1 : Mouse testis lysate
      Lane 2 : Rat testis lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 48 kDa
      Additional bands at: 48 kDa. We are unsure as to the identity of these extra bands.



      This data was developed using ab191429, the same antibody clone in a different buffer formulation.

       

    • Anti-NXN/NRX antibody [EPR15177] - BSA and Azide free (ab251080)
      Anti-NXN/NRX antibody [EPR15177] - BSA and Azide free (ab251080)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

  • References (0)

    Publishing research using ab251080? Please let us know so that we can cite the reference in this datasheet.

    ab251080 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab251080.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2021 Abcam plc. All rights reserved.