Key features and details
- Rabbit polyclonal to NXPH4
- Suitable for: WB, ELISA, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-NXPH4 antibody
DescriptionRabbit polyclonal to NXPH4
Specificityab74999 detects endogenous levels of total NXPH4 protein.
Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Rat
A synthesized peptide derived from an internal region of human NXPH4.
- IHC-P: human brain tissue. WB: extracts from COLO cells and HUVEC cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThe antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
Our Abpromise guarantee covers the use of ab74999 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 33 kDa.|
|IHC-P||1/50 - 1/100.|
RelevanceNXPH4 is a neurexophilin. Mammalian brains contain four different neurexophilin proteins. Neurexophilins form a family of related glycoproteins that are proteolytically processed after synthesis and bind to alpha neurexins. The structure and characteristics of neurexophilins indicate that they function as neuropeptides that may signal via alpha neurexins and possibly other receptors.
- Neurexophilin 4 antibody
- NPH4 antibody
- NXPH 4 antibody
All lanes : Anti-NXPH4 antibody (ab74999) at 1/500 dilution
Lane 1 : Extracts from COLO cells
Lane 2 : Extracts from HUVEC cells
Lane 3 : Extracts from COLO cells plus 5µg immunizing peptide
Lysates/proteins at 5 µg per lane.
Predicted band size: 33 kDa
Observed band size: 33 kDa
Immunohistochemical analysis of NXPH4 in paraffin embedded human brain tissue using ab74999 at a 1/50 dilution.
Left image: untreated.
Right image: treated with immunizing peptide.
ab74999 has not yet been referenced specifically in any publications.