Anti-NXT2 antibody (ab121797)
Key features and details
- Rabbit polyclonal to NXT2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-NXT2 antibody -
Description
Rabbit polyclonal to NXT2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
antigen sequence, corresponding to amino acids 31-142 of Human NXT2.
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Positive control
- Human pancreas tissue; Transfected HEK293T cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab121797 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
Use a concentration of 0.04 - 0.4 µg/ml. |
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Regulator of protein export for NES-containing proteins. Also plays a role in mRNA nuclear export. -
Sequence similarities
Contains 1 NTF2 domain. -
Cellular localization
Nucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 55916 Human
- Omim: 300320 Human
- SwissProt: Q9NPJ8 Human
- Unigene: 25010 Human
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Alternative names
- NTF2-related export protein 2 antibody
- Nxt2 antibody
- NXT2_HUMAN antibody
- Protein p15-2 antibody
Images
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ab121797, at 1/450 dilution, staining NXT2 in paraffin-embedded Human pancreas tissue, by Immunohistochemistry.
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All lanes : Anti-NXT2 antibody (ab121797) at 1/250 dilution
Lane 1 : Negative control (Vector-only transfected HEK293T lysate).
Lane 2 : NXT2-transfected HEK293T Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa))
Developed using the ECL technique.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab121797 has been referenced in 2 publications.
- Malone CF et al. Selective Modulation of a Pan-Essential Protein as a Therapeutic Strategy in Cancer. Cancer Discov 11:2282-2299 (2021). PubMed: 33883167
- Xu L et al. MicroRNA-7-regulated TLR9 signaling-enhanced growth and metastatic potential of human lung cancer cells by altering the phosphoinositide-3-kinase, regulatory subunit 3/Akt pathway. Mol Biol Cell 24:42-55 (2013). WB . PubMed: 23135998