Product nameAnti-NYREN18 antibody [EPR10716]
See all NYREN18 primary antibodies
DescriptionRabbit monoclonal [EPR10716] to NYREN18
Tested applicationsSuitable for: WB, ICC/IF, Flow Cytmore details
Unsuitable for: IHC-P or IP
Species reactivityReacts with: Human
Does not react with: Mouse, Rat
Synthetic peptide within Human NYREN18 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: Q9Y5A7
- Raji, SHSY5Y, 293T and HeLa cell line lysates. HeLa cells. Permeabilized Raji cells.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab171077 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 71 kDa.|
|ICC/IF||1/100 - 1/250.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionSpecific down-regulator of the NEDD8 conjugation system. Recruits NEDD8, UBD, and their conjugates to the proteasome for degradation. Isoform 1 promotes the degradation of NEDD8 more efficiently than isoform 2.
Tissue specificityWidely expressed with lowest expression in the pancreas for isoform 1 and in leukocytes, liver, prostate and skeletal muscle for isoform 2.
Sequence similaritiesContains 3 UBA domains.
Cellular localizationNucleus. Predominantly nuclear.
- Information by UniProt
- BS4 antibody
- NEDD8 ultimate buster 1 antibody
- Negative regulator of ubiquitin like proteins 1 antibody
All lanes : Anti-NYREN18 antibody [EPR10716] (ab171077) at 1/1000 dilution
Lane 1 : Raji cell lysate
Lane 2 : SHSY5Y cell lysate
Lane 3 : 293T cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 71 kDa
Immunofluorescent staining of HeLa cells labeling NUB1 using ab171077 at 1/100 dilution.
Flow cytometric analysis of permeabilized Raji cells labeling NUB1 using ab171077 at 1/10 dilution (red) or a rabbit IgG negative(green).
ab171077 has not yet been referenced specifically in any publications.