Recombinant
RabMAb

Recombinant Anti-O-Linked N-Acetylglucosamine antibody [EPR19847] - BSA and Azide free (ab252384)

Overview

  • Product name

    Anti-O-Linked N-Acetylglucosamine antibody [EPR19847] - BSA and Azide free
    See all O-Linked N-Acetylglucosamine primary antibodies
  • Description

    Rabbit monoclonal [EPR19847] to O-Linked N-Acetylglucosamine - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Dot blot, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human O-Linked N-Acetylglucosamine. The exact sequence is proprietary.

  • Positive control

    • WB: HeLa treated with DMSO (0.4%) as baseline control whole cell lysate; HeLa treated with 200 µM Ac45SGlcNAc for 24 hours whole cell lysate; HeLa treated with 200 µM Thiamet-G for 24 hours whole cell lysate; HEK-293t and HeLa whole cell lysate. IP: HeLa whole cell lysate.
  • General notes

    Ab252384 is the carrier-free version of ab202665. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab252384 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR19847
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab252384 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.
Dot blot Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

Target

  • Relevance

    Many cellular proteins, including nuclear pore, oncogene, cytoskeletal, heat shock, viral and transcription regulatory proteins contain single O-linked N-acetylglucosamine (O-GlcNAc) residues attached to serine or threonine residues. It has been observed that O-GlcNAc glycosylated proteins tend to be under phosphorylated relative to unglycosylated proteins and that O-GlcNAc bearing proteins tend to be found in multimeric complexes. This has led to the suggestion that O-GlcNAc glycosylation may obscure phosphorylation sites and acts as a signaling mechanism or mediator of signaling.

Images

  • Dot Blot - Anti-O-Linked N-Acetylglucosamine antibody [EPR19847] (ab202665 1:1000 dilution).

    Lane 1: O-linked N-acetylglucosamine (O-GlcNAc) peptide 10 ng.

    Lane 2: Non-O-GlcNAc peptide 10 ng.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051 1:100,000 dilution).

    Exposure time of 3 minutes.

    The peptides were kindly provided by our collaborator Dr. Xing Chen, Peking University.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab202665).

  • O-Linked N-Acetylglucosamine was immunoprecipitated from 0.35 mg HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate with ab202665 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab202665 at 1/1,000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.
    Lane 1: HeLa whole cell lysate 10 µg (Input).
    Lane 2: ab202665 IP in HeLa whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab202665 in HeLa whole cell lysate.
    Blocking/Dilution buffer: 5% NFDM/TBST.
    Exposure time: 3 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab202665).

References

ab252384 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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