Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab82666 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 46 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|ICC/IF||Use a concentration of 5 µg/ml.|
FunctionMay play a role in mediating resistance to virus infection, control of cell growth, differentiation, and apoptosis.
Sequence similaritiesBelongs to the 2-5A synthase family.
Cellular localizationMitochondrion. Nucleus. Microsome. Endoplasmic reticulum. Associated with different subcellular fractions such as mitochondrial, nuclear, and rough/smooth microsomal fractions.
- Information by UniProt
- (2 5')oligo(A) synthetase 1 antibody
- (2-5'')oligo(A) synthase 1 antibody
- 2 5 Oligoadenylate Synthetase 1 antibody
Anti-OAS1 antibody (ab82666) at 1 µg/ml (5% skim milk/ PBS buffer) + 293T cell lysate at 10 µg with gel concentration: 12%
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 46 kDa
Observed band size: 46 kDa
Additional bands at: 32 kDa, 35 kDa. We are unsure as to the identity of these extra bands.
ICC/IF image of ab82666 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab82666, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunohistochemistry of Tonsil tissue at an antibody concentration of 5µg/ml using anti-OAS1 antibody (ab82666)