Anti-OASL antibody (ab229136)
Key features and details
- Rabbit polyclonal to OASL
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-OASL antibody
See all OASL primary antibodies -
Description
Rabbit polyclonal to OASL -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee -
Immunogen
Recombinant fragment within Human OASL (internal sequence). The exact sequence is proprietary.
Database link: Q15646 -
Positive control
- WB: HCT 116 whole cell lysate. IHC-P: Human breast cancer tissue. ICC/IF: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab229136 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/100 - 1/1000.
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WB |
1/500 - 1/3000. Predicted molecular weight: 59 kDa.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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IHC-P
1/100 - 1/1000. |
WB
1/500 - 1/3000. Predicted molecular weight: 59 kDa. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Does not have 2'-5'-OAS activity, but can bind double-stranded RNA. Displays antiviral activity against encephalomyocarditis virus (EMCV) and hepatitis C virus (HCV) via an alternative antiviral pathway independent of RNase L. -
Tissue specificity
Expressed in most tissues, with the highest levels in primary blood Leukocytes and other hematopoietic system tissues, colon, stomach and to some extent in testis. -
Sequence similarities
Belongs to the 2-5A synthase family.
Contains 2 ubiquitin-like domains. -
Domain
The ubiquitin-like domains are essential for its antiviral activity. -
Cellular localization
Cytoplasm and Nucleus > nucleolus. Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 8638 Human
- Omim: 603281 Human
- SwissProt: Q15646 Human
- Unigene: 118633 Human
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Alternative names
- 2' 5' oligoadenylate synthetase like antibody
- 2''-5''-OAS-related protein antibody
- 2''-5''-OAS-RP antibody
see all
Images
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Anti-OASL antibody (ab229136) at 1/2000 dilution + HCT116 (human colorectal carcinoma cell line) whole cell lysate at 30 µg
Predicted band size: 59 kDa7.5% SDS-PAGE gel.
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Paraffin-embedded human breast cancer tissue stained for OASL using ab229136 at 1/250 dilution in immunohistochemical analysis.
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Paraformaldehyde-fixed Hela (human epithelial cell line from cervix adenocarcinoma) cells stained for OASL (green) using ab229136 at 1/500 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342 (blue).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab229136 has been referenced in 1 publication.
- Zeng Z et al. Overexpression of OASL upregulates TET1 to induce aberrant activation of CD4+ T cells in systemic sclerosis via IRF1 signaling. Arthritis Res Ther 24:50 (2022). PubMed: 35183246