Key features and details
- Rabbit polyclonal to OASL
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-OASL antibody
See all OASL primary antibodies
DescriptionRabbit polyclonal to OASL
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee
Recombinant fragment within Human OASL (internal sequence). The exact sequence is proprietary.
Database link: Q15646
- WB: HCT 116 whole cell lysate. IHC-P: Human breast cancer tissue. ICC/IF: HeLa cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab229136 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/1000.|
|WB||1/500 - 1/3000. Predicted molecular weight: 59 kDa.|
|ICC/IF||1/100 - 1/1000.|
FunctionDoes not have 2'-5'-OAS activity, but can bind double-stranded RNA. Displays antiviral activity against encephalomyocarditis virus (EMCV) and hepatitis C virus (HCV) via an alternative antiviral pathway independent of RNase L.
Tissue specificityExpressed in most tissues, with the highest levels in primary blood Leukocytes and other hematopoietic system tissues, colon, stomach and to some extent in testis.
Sequence similaritiesBelongs to the 2-5A synthase family.
Contains 2 ubiquitin-like domains.
DomainThe ubiquitin-like domains are essential for its antiviral activity.
Cellular localizationCytoplasm and Nucleus > nucleolus. Cytoplasm.
- Information by UniProt
- 2' 5' oligoadenylate synthetase like antibody
- 2''-5''-OAS-related protein antibody
- 2''-5''-OAS-RP antibody
Anti-OASL antibody (ab229136) at 1/2000 dilution + HCT116 (human colorectal carcinoma cell line) whole cell lysate at 30 µg
Predicted band size: 59 kDa
7.5% SDS-PAGE gel.
Paraffin-embedded human breast cancer tissue stained for OASL using ab229136 at 1/250 dilution in immunohistochemical analysis.
Paraformaldehyde-fixed Hela (human epithelial cell line from cervix adenocarcinoma) cells stained for OASL (green) using ab229136 at 1/500 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342 (blue).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab229136 has not yet been referenced specifically in any publications.