Key features and details
- Rabbit polyclonal to OASL - Carboxyterminal end
- Suitable for: WB, ELISA, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-OASL antibody - Carboxyterminal end
See all OASL primary antibodies
DescriptionRabbit polyclonal to OASL - Carboxyterminal end
SpecificityThis antibody is specific for OASL (C term).
Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
Species reactivityReacts with: Human
A KLH conjugated synthetic peptide (10-30 aa in length) at the C-term of last 50 aa of human OASL.
- HL60 lysate and breast carcinoma tissue. This antibody gave a positive result when used in the following methanol fixed cell lines: HCT116
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab38325 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/100 - 1/500. Detects a band of approximately 60 kDa (predicted molecular weight: 59 kDa).|
|IHC-P||1/50 - 1/100.|
FunctionDoes not have 2'-5'-OAS activity, but can bind double-stranded RNA. Displays antiviral activity against encephalomyocarditis virus (EMCV) and hepatitis C virus (HCV) via an alternative antiviral pathway independent of RNase L.
Tissue specificityExpressed in most tissues, with the highest levels in primary blood Leukocytes and other hematopoietic system tissues, colon, stomach and to some extent in testis.
Sequence similaritiesBelongs to the 2-5A synthase family.
Contains 2 ubiquitin-like domains.
DomainThe ubiquitin-like domains are essential for its antiviral activity.
Cellular localizationCytoplasm and Nucleus > nucleolus. Cytoplasm.
- Information by UniProt
- 2' 5' oligoadenylate synthetase like antibody
- 2''-5''-OAS-related protein antibody
- 2''-5''-OAS-RP antibody
Anti-OASL antibody - Carboxyterminal end (ab38325) at 1/100 dilution + HL60 lysate
Predicted band size: 59 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Additional bands at: 37 kDa, 70 kDa. We are unsure as to the identity of these extra bands.
Formalin-fixed and paraffin-embedded human breast cancer tissue reacted with ab38325 at a 1/50 dilution, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
ab38325 has been referenced in 1 publication.
- Kroeker AL et al. Response of primary human airway epithelial cells to influenza infection: a quantitative proteomic study. J Proteome Res 11:4132-46 (2012). WB . PubMed: 22694362