Overview

  • Product name

    Anti-OATP1B1 antibody [MDQ]
    See all OATP1B1 primary antibodies
  • Description

    Mouse monoclonal [MDQ] to OATP1B1
  • Host species

    Mouse
  • Specificity

    Ab15442 will also cross react with OATP8.
  • Tested applications

    Suitable for: WB, IHC-Fr, ICC/IF, IPmore details
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat, Dog
  • Immunogen

    Synthetic peptide corresponding to Human OATP1B1 aa 1-23.
    Sequence:

    MDQNQHLNKTAEAQPSENKKTRY

  • Positive control

    • Human liver tissue slides.
  • General notes

     This product was previously labelled as OATP2

     

Properties

Applications

Our Abpromise guarantee covers the use of ab15442 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.
IHC-Fr 1/20.
ICC/IF 1/20.
IP Use at an assay dependent concentration.

Target

  • Function

    Mediates the Na(+)-independent uptake of organic anions such as pravastatin, taurocholate, methotrexate, dehydroepiandrosterone sulfate, 17-beta-glucuronosyl estradiol, estrone sulfate, prostaglandin E2, thromboxane B2, leukotriene C3, leukotriene E4, thyroxine and triiodothyronine. May play an important role in the clearance of bile acids and organic anions from the liver.
  • Tissue specificity

    Highly expressed in liver. Not detected in heart, brain, placenta, lung, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, ovary, small intestine, colon and leukocyte.
  • Sequence similarities

    Belongs to the organo anion transporter (TC 2.A.60) family.
    Contains 1 Kazal-like domain.
  • Cellular localization

    Basolateral cell membrane. Detected in basolateral membranes of hepatocytes.
  • Information by UniProt
  • Database links

  • Alternative names

    • Liver-specific organic anion transporter 1 antibody
    • LST-1 antibody
    • LST1 antibody
    • OATP-2 antibody
    • OATP-C antibody
    • OATP1B1 antibody
    • OATPC antibody
    • SLC21A6 antibody
    • SLCO1B1 antibody
    • SO1B1_HUMAN antibody
    • Sodium-independent organic anion-transporting polypeptide 2 antibody
    • Solute carrier family 21 member 6 antibody
    • Solute carrier organic anion transporter family member 1B1 antibody
    see all

Images

  • Immunocytochemistry/Immunofluorescence analysis of HepG2 cells labeling OATP1B1 (green) with ab15542 at 1/20. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue). Cells were fixed with formaldehyde and incubated with the primary antibody overnight at 4°C. A DyLight 488-conjugated secondary antibody was used. 60X magnification. Right - negative control.

References

This product has been referenced in:

  • Imada S  et al. Central bisectionectomy for hepatocellular carcinoma in a patient with indocyanine green excretory defect associated with reduced expression of the liver transporter. Surg Case Rep 2:89 (2016). Read more (PubMed: 27589984) »
  • Peng JF  et al. Role of miR-511 in the Regulation of OATP1B1 Expression by Free Fatty Acid. Biomol Ther (Seoul) 23:400-6 (2015). WB . Read more (PubMed: 26336578) »
See all 3 Publications for this product

Customer reviews and Q&As

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1-2 of 2 Abreviews

Application
Western blot
Sample
Human Cell lysate - whole cell (human HEK293 cells over-expressing)
Loading amount
150 µg
Specification
human HEK293 cells over-expressing
Gel Running Conditions
Reduced Denaturing (denature at 70C for 10mins, 7.5% gel)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Sep 08 2011

Application
Western blot
Sample
Human Cell lysate - other (transiently transfected HeLa cells)
Loading amount
100000 cells
Specification
transiently transfected HeLa cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

Abcam user community

Verified customer

Submitted Dec 19 2006

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