Question (14583) | Anti-OATP1B1 antibody [MDQ] (ab15442)

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Question

How exactly were these antibodies tested in IP, IHC, and IF?

Answer

Thank you for your enquiry. Both antibodies were tested in IP using human liver samples (~20-50 ug) and about 1 ug/ml of antibody used. For IF, human liver and transfected HEK293 whole cell lysates were used. Ab15442 was used at a dilution of 1:20. The fixative was 4% PFA for ~10-15 minutes. Ab15441 was used at 1:100 in IF. If you have any additional questions, please contact us again.

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