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How exactly were these antibodies tested in IP, IHC, and IF?
Asked on Nov 02 2005
Thank you for your enquiry. Both antibodies were tested in IP using human liver samples (~20-50 ug) and about 1 ug/ml of antibody used. For IF, human liver and transfected HEK293 whole cell lysates were used. Ab15442 was used at a dilution of 1:20. The fixative was 4% PFA for ~10-15 minutes. Ab15441 was used at 1:100 in IF. If you have any additional questions, please contact us again.
Answered on Nov 04 2005