Overview

  • Product name
    Anti-OATP2 antibody [MDQ]
    See all OATP2 primary antibodies
  • Description
    Mouse monoclonal [MDQ] to OATP2
  • Host species
    Mouse
  • Specificity
    Ab15442 will also cross react with OATP8.
  • Tested applications
    Suitable for: WB, IHC-Fr, ICC/IF, IPmore details
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat, Dog
  • Immunogen

    Synthetic peptide corresponding to Human OATP2 aa 1-23.
    Sequence:

    MDQNQHLNKTAEAQPSENKKTRY

  • Positive control
    • Human liver tissue slides.

Properties

Applications

Our Abpromise guarantee covers the use of ab15442 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.
IHC-Fr 1/20.
ICC/IF 1/20.
IP Use at an assay dependent concentration.

Target

  • Function
    Mediates the Na(+)-independent uptake of organic anions such as pravastatin, taurocholate, methotrexate, dehydroepiandrosterone sulfate, 17-beta-glucuronosyl estradiol, estrone sulfate, prostaglandin E2, thromboxane B2, leukotriene C3, leukotriene E4, thyroxine and triiodothyronine. May play an important role in the clearance of bile acids and organic anions from the liver.
  • Tissue specificity
    Highly expressed in liver. Not detected in heart, brain, placenta, lung, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, ovary, small intestine, colon and leukocyte.
  • Sequence similarities
    Belongs to the organo anion transporter (TC 2.A.60) family.
    Contains 1 Kazal-like domain.
  • Cellular localization
    Basolateral cell membrane. Detected in basolateral membranes of hepatocytes.
  • Information by UniProt
  • Database links
  • Alternative names
    • Liver-specific organic anion transporter 1 antibody
    • LST-1 antibody
    • LST1 antibody
    • OATP-2 antibody
    • OATP-C antibody
    • OATP1B1 antibody
    • OATPC antibody
    • SLC21A6 antibody
    • SLCO1B1 antibody
    • SO1B1_HUMAN antibody
    • Sodium-independent organic anion-transporting polypeptide 2 antibody
    • Solute carrier family 21 member 6 antibody
    • Solute carrier organic anion transporter family member 1B1 antibody
    see all

Images

  • Immunocytochemistry/Immunofluorescence analysis of HepG2 cells labeling OATP2 (green) with ab15542 at 1/20. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue). Cells were fixed with formaldehyde and incubated with the primary antibody overnight at 4°C. A DyLight 488-conjugated secondary antibody was used. 60X magnification. Right - negative control.

References

This product has been referenced in:
  • Imada S  et al. Central bisectionectomy for hepatocellular carcinoma in a patient with indocyanine green excretory defect associated with reduced expression of the liver transporter. Surg Case Rep 2:89 (2016). Read more (PubMed: 27589984) »
  • Peng JF  et al. Role of miR-511 in the Regulation of OATP1B1 Expression by Free Fatty Acid. Biomol Ther (Seoul) 23:400-6 (2015). WB . Read more (PubMed: 26336578) »
See all 3 Publications for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Answer



Any products that require an MSDS form will have a link on the product's online datasheet to the data. It will be a PDF document link labeled 'SDS'. Please see attached image for example of what to look for on the datasheet. If no 'SDS' link is available than the product does not contain any components that require an SDS.

Read More
Application
Western blot
Sample
Human Cell lysate - whole cell (human HEK293 cells over-expressing)
Loading amount
150 µg
Specification
human HEK293 cells over-expressing
Gel Running Conditions
Reduced Denaturing (denature at 70C for 10mins, 7.5% gel)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Sep 08 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - other (transiently transfected HeLa cells)
Loading amount
100000 cells
Specification
transiently transfected HeLa cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

Abcam user community

Verified customer

Submitted Dec 19 2006

Answer

Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. I have read through your technical questionaire and I have a few comments. OATP2 antibody [MDQ] (ab15442) has been tested for its applications of immunofluorescence, immunohistochemistry (Frozen sections) and immunoprecipitation. To our knowledge we are not aware of how this antibody behaves in western blotting. Therefore unfortunately we cannot guarantee it for this purpose. I am sorry to hear that you have observed differences between the batches of antibody that you have used. This is a monoclonal antibody and I would not expect different antibody preparations to have significant variation in their quality. However, occasional batch to batch variation does exist. Therefore given this I would like to recommend that you perform the optimisation to your protocol; Firstly I would like to suggest that you verify the integrity of your sample and preparation by performing a loading control immunolabelling using an antibody that targets a housekeeping protein such as GAPDH or alpha tubulin. Following on I would like to suggest that you significantly reduce the dilution of the antibody to ~1:20 in view of the antibody dilutions recommended for immunofluorescence and perform an overnight incubation at 4oC using BSA as a blocking agent. This serves to improve the sensitivity of the antibody.

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Question
Answer

Thank you for your enquiry. Both antibodies were tested in IP using human liver samples (~20-50 ug) and about 1 ug/ml of antibody used. For IF, human liver and transfected HEK293 whole cell lysates were used. Ab15442 was used at a dilution of 1:20. The fixative was 4% PFA for ~10-15 minutes. Ab15441 was used at 1:100 in IF. If you have any additional questions, please contact us again.

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Answer

Thank you for bringing this to our attention. The immunogen sequence is from OATP2, however this product will also recognise OATP8. The data sheet has now been updated to reflect this. Please let me know if you spot any further problems. I apologize for any inconvenience that this may have caused.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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