Anti-OATP1B1 antibody [MDQ] (ab15442)
Key features and details
- Mouse monoclonal [MDQ] to OATP1B1
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-OATP1B1 antibody [MDQ]
See all OATP1B1 primary antibodies -
Description
Mouse monoclonal [MDQ] to OATP1B1 -
Host species
Mouse -
Specificity
Ab15442 will also cross react with OATP8. -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat, Dog -
Immunogen
Synthetic peptide corresponding to Human OATP1B1 aa 1-23.
Sequence:MDQNQHLNKTAEAQPSENKKTRY
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Positive control
- Human liver tissue slides.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
MDQ -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab15442 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/20.
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Notes |
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ICC/IF
1/20. |
Target
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Function
Mediates the Na(+)-independent uptake of organic anions such as pravastatin, taurocholate, methotrexate, dehydroepiandrosterone sulfate, 17-beta-glucuronosyl estradiol, estrone sulfate, prostaglandin E2, thromboxane B2, leukotriene C3, leukotriene E4, thyroxine and triiodothyronine. May play an important role in the clearance of bile acids and organic anions from the liver. -
Tissue specificity
Highly expressed in liver. Not detected in heart, brain, placenta, lung, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, ovary, small intestine, colon and leukocyte. -
Sequence similarities
Belongs to the organo anion transporter (TC 2.A.60) family.
Contains 1 Kazal-like domain. -
Cellular localization
Basolateral cell membrane. Detected in basolateral membranes of hepatocytes. - Information by UniProt
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Database links
- Entrez Gene: 10599 Human
- Omim: 604843 Human
- SwissProt: Q9Y6L6 Human
- Unigene: 449738 Human
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Alternative names
- Liver-specific organic anion transporter 1 antibody
- LST-1 antibody
- LST1 antibody
see all
Images
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Immunocytochemistry/Immunofluorescence analysis of HepG2 cells labeling OATP1B1 (green) with ab15542 at 1/20. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue). Cells were fixed with formaldehyde and incubated with the primary antibody overnight at 4°C. A DyLight 488-conjugated secondary antibody was used. 60X magnification. Right - negative control.
Protocols
Datasheets and documents
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Datasheet download
References (3)
ab15442 has been referenced in 3 publications.
- Imada S et al. Central bisectionectomy for hepatocellular carcinoma in a patient with indocyanine green excretory defect associated with reduced expression of the liver transporter. Surg Case Rep 2:89 (2016). PubMed: 27589984
- Peng JF et al. Role of miR-511 in the Regulation of OATP1B1 Expression by Free Fatty Acid. Biomol Ther (Seoul) 23:400-6 (2015). WB . PubMed: 26336578
- Sticova E et al. Down-regulation of OATP1B proteins correlates with hyperbilirubinemia in advanced cholestasis. Int J Clin Exp Pathol 8:5252-62 (2015). PubMed: 26191226