Overview

  • Product name

    Anti-OATP5A1 antibody [EPR14395(2)]
    See all OATP5A1 primary antibodies
  • Description

    Rabbit monoclonal [EPR14395(2)] to OATP5A1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment within Human OATP5A1 aa 500-650. The exact sequence is proprietary.
    Database link: Q9H2Y9

  • Positive control

    • MCF7, T47D cell lysates; Human fetal brain and fetal heart tissue lysates; Human and Mouse kidney tissues
  • General notes

     

     

     This product was previously labelled as SLCO5A1

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR14395(2)
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab191412 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 92 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Sequence similarities

    Belongs to the organo anion transporter (TC 2.A.60) family.
    Contains 1 Kazal-like domain.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Form

    Organic anion transporters, such as SLCO5A1, are 12-pass transmembrane proteins that transport a wide spectrum of amphipathic organic solutes apparently via an anion exchange mechanism.
  • Alternative names

    • FLJ39560 antibody
    • OATP-J antibody
    • OATP-RP4 antibody
    • OATP5A1 antibody
    • OATPRP4 antibody
    • Organic anion transporter polypeptide-related protein 4 antibody
    • SLC21A15 antibody
    • SLCO5A1 antibody
    • SO5A1_HUMAN antibody
    • solute carrier family 21 (organic anion transporter), member 15 antibody
    • Solute carrier family 21 member 15 antibody
    • Solute carrier organic anion transporter family member 5A1 antibody
    see all

Images

  • All lanes : Anti-OATP5A1 antibody [EPR14395(2)] (ab191412) at 1/1000 dilution

    Lane 1 : MCF7 cell lysate
    Lane 2 : T47D cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 92 kDa

  • All lanes : Anti-OATP5A1 antibody [EPR14395(2)] (ab191412) at 1/10000 dilution

    Lane 1 : Human fetal brain tissue lysate
    Lane 2 : Human fetal heart tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 92 kDa

  • Immunohistochemical analysis of paraffin-embedded Human fetal kidney tissue labeling OATP5A1 using ab191412 at 1/100 dilution (10μg/mL).  A Ready to use HRP Polymer for Rabbit IgG (prediluted) was used as secondary. Counterstain: Hematoxylin. Inset image: negative control obtained using PBS instead of ab191412.

  • Immunohistochemical analysis of paraffin-embedded Mouse fetal kidney tissue labeling OATP5A1 using ab191412 at 1/100 dilution (10 μg/mL). A Ready to use HRP Polymer for Rabbit IgG (prediluted) was used as secondary. Counterstain: Hematoxylin. Inset image: negative control obtained using PBS instead of ab191412.

References

ab191412 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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