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Rabbit polyclonal to OAZ1 - N-terminal
Synthetic peptide selected from the N terminal region of Human OAZ1, conjugated to KLH (NP_004143).
Mouse heart tissue lysate.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Concentration information loading...
Ammonium Sulphate Precipitation
ab85221 is prepared by Saturated Ammonium Sulphate (SAS) precipitation followed by dialysis against PBS.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/1000. Predicted molecular weight: 25 kDa.
Binds to, and destabilizes, ornithine decarboxylase which is then degraded. Also inhibits cellular uptake of polyamines by inactivating the polyamine uptake transporter. SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1.
Belongs to the ODC antizyme family.
Information by UniProt
Antizyme 1 antibody
Western blot - Anti-OAZ1 antibody - N-terminal (ab85221)
Anti-OAZ1 antibody - N-terminal (ab85221) at 1/1000 dilution + Mouse heart tissue lysate at 35 µg
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size:
Observed band size:
28 kDa (
why is the actual band size different from the predicted?
Additional bands at:
16 kDa, 40 kDa. We are unsure as to the identity of these extra bands.
Blocking/Dilution buffer: 5% NFDM/TBST.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"