Overview

  • Product name

  • Description

    Rabbit polyclonal to OB Cadherin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Chicken, Human
    Predicted to work with: Rat, Cow
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 65-343 of Human OB Cadherin (Uniprot ID: P55287)

  • Positive control

    • Mouse brain lysate; Normal Human lung tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab151302 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 87 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types.
  • Tissue specificity

    Expressed mainly in brain but also found in other tissues. Expressed in neuroblasts.
  • Involvement in disease

    Note=A chromosomal aberration involving CDH11 is a common genetic feature of aneurysmal bone cyst, a benign osseous neoplasm. Translocation t(16;17)(q22;p13) with USP6. The translocation generates a fusion gene in which the strong CDH11 promoter is fused to the entire USP6 coding sequence, resulting in USP6 transcriptional up-regulation.
  • Sequence similarities

    Contains 5 cadherin domains.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CAD11 antibody
    • CAD11_HUMAN antibody
    • Cadherin 11 antibody
    • Cadherin 11 type 2 antibody
    • Cadherin 11 type 2 OB cadherin (osteoblast) antibody
    • Cadherin-11 antibody
    • Cdh11 antibody
    • CDHOB antibody
    • OB antibody
    • OB-cadherin antibody
    • OBcadherin antibody
    • OSF-4 antibody
    • OSF4 antibody
    • Osteoblast cadherin antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded normal Human lung tissue labeling OB Cadherin with ab151302 antibody at 1/250 dilution.
  • Anti-OB Cadherin antibody (ab151302) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg

    Predicted band size: 87 kDa



    5% SDS PAGE

References

This product has been referenced in:

  • Ueda Y  et al. Additive effects of inhibiting both mTOR and glutamine metabolism on the arthritis in SKG mice. Sci Rep 9:6374 (2019). Read more (PubMed: 31011190) »
  • Luan L  et al. HOXD10 silencing suppresses human fibroblast-like synoviocyte migration in rheumatoid arthritis via downregulation of the p38/JNK pathway. Exp Ther Med 16:1621-1628 (2018). Read more (PubMed: 30186380) »
See all 4 Publications for this product

Customer reviews and Q&As

Filter by Application

Filter by Species

Filter by Ratings

1-3 of 3 Abreviews

Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
Sample
Chicken Tissue sections (Hindbrain section)
Specification
Hindbrain section
Permeabilization
Yes - PBS + 0.1% Tween20
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jan 15 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer pH6
Sample
Mouse Tissue sections (Ela::Myc transgenic mice)
Specification
Ela::Myc transgenic mice
Permeabilization
No
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Jun 12 2013

Application
Western blot
Sample
Mouse Cell lysate - whole cell (MEFs)
Loading amount
50 µg
Specification
MEFs
Treatment
TGF-b 5ng/ml for 48h
Gel Running Conditions
Reduced Denaturing (7.5% gel)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Apr 02 2013

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up