Western blot abreview for Anti-OB Cadherin antibody

Excellent
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - whole cell (MEFs)
Loading amount
50 µg
Specification
MEFs
Treatment
TGF-b 5ng/ml for 48h
Gel Running Conditions
Reduced Denaturing (7.5% gel)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Other product details

Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS-T 1%Milk

Secondary antibody

Name
Non-Abcam antibody was used: DAKO anti Rb
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000

Detection

Detection method
ECL
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 150 kDa

Additional data

Additional Notes
As the images in antibody data sheet OB-Cadh do not appear at predicted MW, instead of it, antibody recognices an 150kDa MW band, probably due to a covalent dimer formation.

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Submitted Apr 02 2013

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