Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Good
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer pH6
Sample
Mouse Tissue sections (Ela::Myc transgenic mice)
Specification
Ela::Myc transgenic mice
Permeabilization
No
Fixative
Formaldehyde

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS-T 1% FBS
Dilution
1/100

Secondary antibody

Name
Non-Abcam antibody was used: Leyca envision anti-Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Additional data

Additional Notes
Althought epithelial staining seems specific, the antibody seems to recognice non-specfic areas as backgroud.

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Submitted Jun 12 2013

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