Anti-Obestatin antibody (ab41704)

Abreviews (1)Q&A (2)

Overview

  • Product name
    Anti-Obestatin antibody
    See all Obestatin primary antibodies
  • Description
    Rabbit polyclonal to Obestatin
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep
    Predicted to work with: Cat, Dog, Human, Macaque monkey, Gerbil
  • Immunogen

    Synthetic peptide corresponding to Rat Obestatin aa 1-23.
    Sequence:

    FNAPFDVGIKLSGAQYQQHGRAL


    (Peptide available as ab41821)

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

Properties

Applications

Our Abpromise guarantee covers the use of ab41704 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 2.5 kDa (predicted molecular weight: 2.5 kDa).Can be blocked with Rat Obestatin peptide (ab41821).

Target

  • Relevance
    Obestatin, is generated from the proteolytic cleavage of preproghrelin; its sequence corresponds to aamino acids 76-98 of Appetite-regulating hormone [Precursor](Q9QYH7). This peptide has contrasting actions to Ghrelin (a different product of proteolytic cleavage of preproghrelin) in that it inhibits food intake and gastrointestinal motility but does not modify in vitro GH release from pituitary cells.
  • Cellular localization
    Secreted
  • Database links
    • Alternative names
      • Appetite-regulating hormone antibody
      • Growth hormone secretagogue antibody
      • Growth hormone-releasing peptide antibody
      • Motilin-related peptide antibody
      • obestatin 13 antibody
      • Obestatin 23 antibody
      • obestatin-13 antibody
      • Obestatin-23 antibody
      • obestatin13 antibody
      • Obestatin23 antibody
      see all

    Images

    • IHC - Wholemount - Anti-Obestatin antibody (ab41704)
      IHC - Wholemount - Anti-Obestatin antibody (ab41704)This image is courtesy of an Abreview submitted by Michal Szlis

      IHC - Wholemount of sheep duodenum tissue labelling Obestatin with ab41704. Sample was incubated with primary antibody (1/400) at 4°C. ab6795, a HRP-conjugated sheep anti-rabbit polyclonal (1/800) was used as the secondary antibody.

      See Abreview

    References

    ab41704 has not yet been referenced specifically in any publications.

    Publishing research using ab41704? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    3 reviews or Q&A

    10
    Votes: Highest First

    IHC - Wholemount abreview for Anti-Obestatin antibody

     Excellent
    Abreviews
    Application
    IHC - Wholemount
    Sample
    Sheep Tissue (Duodenum)
    Specification
    Duodenum
    Read More
    abreview image
    Username

    Michal Szlis

    Verified customer

    Submitted Jan 30 2014

    What species and tissue samples were used to characterise the anti-Obestatin in Western Blot?

    Les tissus qui ont été utilisés avec le ab41704 sont : Pour le rat : estomac, cerveau, foie, poumons, muscle et reins. Pour la souris : cerveau, intestin grêle et foie. Les meilleurs résultats ont été obtenus avec le cerveau (souris et rat). ...

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    1) What species and tissue samples were used to characterise the anti-Obestatin in Western Blot? 2) Does the anti-Obestatin antibody recognise the precusor?

    Un collaborateur du laboratoire vient de m'informer que le western blot dont il dispose actuellement a été obtenu avec le peptide synthétique uniquement; peptide correspondant à l'obestatine-23 de rat (forme clivée). Cette personne m'a confirmé que ...

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