Anti-Obestatin antibody (ab41704)

Reviews (1)Q&A (2)

Overview

  • Product name

    Anti-Obestatin antibody
    See all Obestatin primary antibodies

  • Description

    Rabbit polyclonal to Obestatin

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Sheep
    Predicted to work with: Cat, Dog, Human, Macaque monkey, Gerbil

  • Immunogen

    Synthetic peptide corresponding to Rat Obestatin aa 1-23.
    Sequence:

    FNAPFDVGIKLSGAQYQQHGRAL


    (Peptide available as ab41821)

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

Properties

Applications

Our Abpromise guarantee covers the use of ab41704 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 2.5 kDa (predicted molecular weight: 2.5 kDa).Can be blocked with Rat Obestatin peptide (ab41821).

Target

  • Relevance

    Obestatin, is generated from the proteolytic cleavage of preproghrelin; its sequence corresponds to aamino acids 76-98 of Appetite-regulating hormone [Precursor](Q9QYH7). This peptide has contrasting actions to Ghrelin (a different product of proteolytic cleavage of preproghrelin) in that it inhibits food intake and gastrointestinal motility but does not modify in vitro GH release from pituitary cells.

  • Cellular localization

    Secreted

  • Database links

    • Alternative names

      • Appetite-regulating hormone antibody
      • Growth hormone secretagogue antibody
      • Growth hormone-releasing peptide antibody
      • Motilin-related peptide antibody
      • obestatin 13 antibody
      • Obestatin 23 antibody
      • obestatin-13 antibody
      • Obestatin-23 antibody
      • obestatin13 antibody
      • Obestatin23 antibody
      see all

    References

    ab41704 has not yet been referenced specifically in any publications.

    Publishing research using ab41704? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1-3 of 3 Abreviews or Q&A

    IHC - Wholemount abreview for Anti-Obestatin antibody

     Excellent
    Abreviews
    abreview image
    Application
    IHC - Wholemount
    Sample
    Sheep Tissue (Duodenum)
    Specification
    Duodenum
    Read More

    Michal Szlis

    Verified customer

    Submitted Jan 30 2014

    Question

    What species and tissue samples were used to characterise the anti-Obestatin in Western Blot?

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    Answer

    Les tissus qui ont été utilisés avec le ab41704 sont : Pour le rat : estomac, cerveau, foie, poumons, muscle et reins. Pour la souris : cerveau, intestin grêle et foie. Les meilleurs résultats ont été obtenus avec le cerveau (souris et rat). J'espère que ces informations vous seront utiles. N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions.

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    Question

    1) What species and tissue samples were used to characterise the anti-Obestatin in Western Blot? 2) Does the anti-Obestatin antibody recognise the precusor?

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    Answer

    Un collaborateur du laboratoire vient de m'informer que le western blot dont il dispose actuellement a été obtenu avec le peptide synthétique uniquement; peptide correspondant à l'obestatine-23 de rat (forme clivée). Cette personne m'a confirmé que cet anticorps a également été testé sur échantillons de souris et de rat. J'ai demandé des détails sur les tissus ayant été testés, je vous enverrai cette information dès que j'en aurai pris connaissance. Puisque cet anticorps reconnait l'obestatine-23 (aa 76-98 de la référence SwissProt/UniProt Q9QYH7), je pense que cet anticorps peut donc également reconnaitre le précurseur long de 117 acides aminés. J'espère que ces informations vous seront utiles. N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions.

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