Recombinant
RabMAb

Recombinant Anti-Obestatin antibody [EPR19972] (ab216575)

Overview

  • Product name

    Anti-Obestatin antibody [EPR19972]
    See all Obestatin primary antibodies
  • Description

    Rabbit monoclonal [EPR19972] to Obestatin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, IHC-Frmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Positive control

    • WB: GST and His-tagged human Appetite-regulating hormone, full length recombinant protein (aa1-117); His-tagged human Obestatin peptide (aa76-98)*5. IHC-P: Human stomach and duodenum tissues; Mouse stomach tissue. IHC-Fr: Mouse stomach tissue.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR19972
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab216575 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 13 kDa (predicted molecular weight: 13 kDa).
IHC-P 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC-Fr 1/500.

Antigen retrieval: Heated citrate solution (10mM citrate PH 6.0 + 0.05% Tween-20).

Target

  • Relevance

    Obestatin, is generated from the proteolytic cleavage of preproghrelin; its sequence corresponds to aamino acids 76-98 of Appetite-regulating hormone [Precursor](Q9QYH7). This peptide has contrasting actions to Ghrelin (a different product of proteolytic cleavage of preproghrelin) in that it inhibits food intake and gastrointestinal motility but does not modify in vitro GH release from pituitary cells.
  • Cellular localization

    Secreted
  • Database links

  • Alternative names

    • Appetite-regulating hormone antibody
    • Growth hormone secretagogue antibody
    • Growth hormone-releasing peptide antibody
    • Motilin-related peptide antibody
    • obestatin 13 antibody
    • Obestatin 23 antibody
    • obestatin-13 antibody
    • Obestatin-23 antibody
    • obestatin13 antibody
    • Obestatin23 antibody
    see all

Images

  • All lanes : Anti-Obestatin antibody [EPR19972] (ab216575) at 1/1000 dilution

    Lane 1 : GST and His-tagged human Appetite-regulating hormone, full length recombinant protein (aa1-117) at 0.005 µg
    Lane 2 : His-tagged human Obestatin peptide (aa76-98)*5 at 0.005 µg
    Lane 3 : His-tagged human Ghrelin peptide (aa24-51)*4 at 0.05 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 13 kDa
    Observed band size: 13,38 kDa
    why is the actual band size different from the predicted?


    Exposure time: 1 second


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Obestatin and Ghrelin are processed cleaved forms deriving from Appetite-regulating hormone. This product is specific to Obestatin.

    Detecting Obestatin in endogenous tissues may require optimization.

  • Immunohistochemical analysis of paraffin-embedded human stomach tissue labeling Obestatin with ab216575 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) at a ready to use dilution. 

    Cytoplasmic staining on endocrine cells of human stomach is observed [PMID: 18474938].

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) at a ready to use dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded human duodenum tissue labeling Obestatin with ab216575 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) at a ready to use dilution. 

    Cytoplasmic staining on endocrine cells of human duodenum is observed [PMID: 18474938].

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded mouse stomach tissue labeling Obestatin with ab216575 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) at a ready to use dilution. 

    Cytoplasmic staining on endocrine cells of mouse stomach is observed [PMID: 18474938].

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) at a ready to use dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded human colon tissue labeling Obestatin with ab216575 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) at a ready to use dilution.

    Negative control: no staining on human colon [PMID: 18474938].

    Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse stomach tissue labeling Obestatin with ab216575 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Cytoplasmic staining on endocrine cells of mouse stomach is observed PMID: 18474938]. 

    The nuclear counterstain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

References

ab216575 has not yet been referenced specifically in any publications.

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