• Product name

  • Description

    Mouse polyclonal to OBFC1
  • Host species

  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Full length human OBFC1 protein (NP_079204.1).

  • Positive control

    • Human spleen tissue lysate, HeLa cell lysate and OBFC1 transfected 293T cell lysate



Our Abpromise guarantee covers the use of ab89250 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 42 kDa.


  • Function

    Component of the CST complex, a complex that binds to single-stranded DNA and is required to protect telomeres from DNA degradation. The CST complex binds single-stranded DNA with high affinity in a sequence-independent manner, while isolated subunits bind DNA with low affinity by themselves. In addition to telomere protection, the CST complex has probably a more general role in DNA metabolism at non-telomeric sites.
  • Sequence similarities

    Belongs to the STN1 family.
    Contains 1 OB DNA-binding domain.
  • Cellular localization

    Nucleus. Chromosome > telomere.
  • Information by UniProt
  • Database links

  • Alternative names

    • AAF 44 antibody
    • AAF44 antibody
    • Alpha accessory factor 44 antibody
    • bA541N10.2 antibody
    • CST complex subunit STN1 antibody
    • FLJ22559 antibody
    • Obfc1 antibody
    • Oligonucleotide/oligosaccharide binding fold containing 1 antibody
    • Oligonucleotide/oligosaccharide-binding fold-containing protein 1 antibody
    • OTTHUMP00000020417 antibody
    • OTTHUMP00000020418 antibody
    • Replication protein A 32 kDa subunit antibody
    • RP11-541N10.2 antibody
    • RPA 32 antibody
    • STN1 antibody
    • STN1_HUMAN antibody
    • Suppressor of cdc thirteen antibody
    • Suppressor of cdc thirteen homolog antibody
    see all


  • Anti-OBFC1 antibody (ab89250) at 1 µg/ml + human spleen tissue lysate at 50 µg

    Predicted band size: 42 kDa
    Observed band size: 42 kDa

  • Anti-OBFC1 antibody (ab89250) at 1 µg/ml + HeLa cell lysate at 50 µg

    Predicted band size: 42 kDa
    Observed band size: 42 kDa

  • All lanes : Anti-OBFC1 antibody (ab89250) at 1 µg/ml

    Lane 1 : OBFC1 transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Predicted band size: 42 kDa
    Observed band size: 42 kDa


This product has been referenced in:

  • Zhang M  et al. Mammalian CST averts replication failure by preventing G-quadruplex accumulation. Nucleic Acids Res 47:5243-5259 (2019). Read more (PubMed: 30976812) »
  • Stewart JA  et al. Human CST promotes telomere duplex replication and general replication restart after fork stalling. EMBO J 31:3537-49 (2012). Read more (PubMed: 22863775) »
See all 2 Publications for this product

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