Overview

  • Product name

    Anti-OC-3 antibody [10B2F5]
    See all OC-3 primary antibodies
  • Description

    Mouse monoclonal [10B2F5] to OC-3
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Recombinant fragment corresponding to Human OC-3 aa 350-455. Expressed in E.coli.
    Sequence:

    C RSQGTLSDLL RNPKPWSKLK SGRETFRRMW KWLQEPEFQR MSALRLAACK RKEQEQQKER ALQPKKQRLV FTDLQRRTLI AIFKENKRPS KEMQVTISQQ LGLEL


    Database link: Q60422

  • Positive control

    • Recombinant Human OC-3 protein; OC-3-transfected HEK293 cell lysate; Human cervical cancer tissue; Human rectum cancer tissue.
  • General notes

    Previously labelled as ONECUT3. 

Properties

Applications

Our Abpromise guarantee covers the use of ab181450 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 50 kDa.
IHC-P 1/200 - 1/1000.

Target

  • Function

    Transcriptional activator. Binds the consensus DNA sequence 5'-DHWATTGAYTWWD-3' on a variety of gene promoters such as those of HNF3B and TTR.
  • Sequence similarities

    Contains 1 CUT DNA-binding domain.
    Contains 1 homeobox DNA-binding domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • OC 3 antibody
    • OC-3 antibody
    • OC3 antibody
    • One cut domain family member 3 antibody
    • One cut homeobox 3 antibody
    • ONEC3_HUMAN antibody
    • ONECUT 3 antibody
    • Onecut3 antibody
    • Transcription factor ONECUT 3 antibody
    • Transcription factor ONECUT-3 antibody
    • Transcription factor ONECUT3 antibody
    see all

Images

  • All lanes : Anti-OC-3 antibody [10B2F5] (ab181450) at 1/500 dilution

    Lane 1 : HEK293 cell lysate
    Lane 2 : OC-3-transfected HEK293 cell lysate

    Predicted band size: 50 kDa

  • Anti-OC-3 antibody [10B2F5] (ab181450) at 1/500 dilution + recombinant Human ONECUT3 protein

    Predicted band size: 50 kDa

  • Immunohistochemical analysis of paraffin-embedded Human rectum cancer tissue labeling OC-3 with ab181450 at a 1/200 dilution.

  • Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissue labeling OC-3 with ab181450 at a 1/200 dilution.

References

ab181450 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Mouse embryonic brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako Ph 6.1
Permeabilization
Yes - Triton
Specification
Mouse embryonic brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Temperature: RT°C
Fixative
Formaldehyde

Dr. Erik Keimpema

Verified customer

Submitted Nov 07 2018

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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