Key features and details
- Mouse monoclonal [10B2F5] to OC-3
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-OC-3 antibody [10B2F5]
See all OC-3 primary antibodies
DescriptionMouse monoclonal [10B2F5] to OC-3
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
- Recombinant Human OC-3 protein; OC-3-transfected HEK293 cell lysate; Human cervical cancer tissue; Human rectum cancer tissue.
Previously labelled as ONECUT3.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituent: 99% PBS
0.5% protein stabilizer consisting of amino acid =85% pH(1% water solution) =7.0~7.5 Water =2% As(mg/kg) =0.5 Pb(mg/kg) =0.1
Concentration information loading...
PurityProtein G purified
Purification notesPurified from tissue culture supernatant.
Our Abpromise guarantee covers the use of ab181450 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Predicted molecular weight: 50 kDa.|
|IHC-P||1/200 - 1/1000.|
FunctionTranscriptional activator. Binds the consensus DNA sequence 5'-DHWATTGAYTWWD-3' on a variety of gene promoters such as those of HNF3B and TTR.
Sequence similaritiesContains 1 CUT DNA-binding domain.
Contains 1 homeobox DNA-binding domain.
- Information by UniProt
- OC 3 antibody
- OC-3 antibody
- OC3 antibody
All lanes : Anti-OC-3 antibody [10B2F5] (ab181450) at 1/500 dilution
Lane 1 : HEK293 cell lysate
Lane 2 : OC-3-transfected HEK293 cell lysate
Predicted band size: 50 kDa
Anti-OC-3 antibody [10B2F5] (ab181450) at 1/500 dilution + recombinant Human ONECUT3 protein
Predicted band size: 50 kDa
Immunohistochemical analysis of paraffin-embedded Human rectum cancer tissue labeling OC-3 with ab181450 at a 1/200 dilution.
Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissue labeling OC-3 with ab181450 at a 1/200 dilution.
ab181450 has not yet been referenced specifically in any publications.