Key features and details
- Rabbit polyclonal to OCC1
- Suitable for: ICC/IF, WB, ELISA, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-OCC1 antibody
DescriptionRabbit polyclonal to OCC1
Tested applicationsSuitable for: ICC/IF, WB, ELISA, IHC-Pmore details
Species reactivityReacts with: Human
16 amino acid peptide designed close to the N terminal region of Human OCC1
- Human spleen tissue lysate.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab83948 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 20 µg/ml.|
|WB||Use a concentration of 2 µg/ml. Predicted molecular weight: 6 kDa.|
|ELISA||Use at an assay dependent concentration.|
|IHC-P||Use a concentration of 2.5 µg/ml.|
Tissue specificityHigh expression in placenta, skeletal muscle, kidney and pancreas tissues. Absent or very faint expression in heart, brain, lung and liver. Expressed during adipogenic differentiation of mesenchymal stem cells (at protein level).
Sequence similaritiesBelongs to the OCC1 family.
- Information by UniProt
- Adipogenesis down regulated 3 antibody
- AGD3 antibody
- C12orf75 antibody
Anti-OCC1 antibody (ab83948) at 2 µg/ml + Human spleen tissue lysate at 15 µg
Predicted band size: 6 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?
Note: in vitro transcription and translation of the OCC1 cDNA yields polypeptides of less than 9 kDa. Despite this, OCC1 from cell lines is detected at ~28 kDa in SDS-PAGE.
Immunofluorescence of OCC1 in Human spleen tissue with ab83948 at 20 ug/mL.
ab83948 at 2.5µg/ml staining OCC1 in human spleen tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections).
ab83948 has been referenced in 1 publication.
- Lan Y et al. Long noncoding RNA OCC-1 suppresses cell growth through destabilizing HuR protein in colorectal cancer. Nucleic Acids Res 46:5809-5821 (2018). PubMed: 29931370