Key features and details
- Rabbit polyclonal to Occludin
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Occludin antibody
See all Occludin primary antibodies
DescriptionRabbit polyclonal to Occludin
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Pig
Synthetic peptide within Mouse Occludin aa 480-520 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: Q61146
- WB: A549 cell lysate. IHC-P: Mouse embryo and kidney tissues.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 1% BSA, 50% Glycerol
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab222691 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/100 - 1/1000. Predicted molecular weight: 59 kDa.|
|IHC-P||1/100 - 1/500.
(or 1/50 - 1/200 if using a fluorescent secondary antibody).
FunctionMay play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. It is able to induce adhesion when expressed in cells lacking tight junctions.
Tissue specificityLocalized at tight junctions of both epithelial and endothelial cells. Highly expressed in kidney. Not detected in testis.
Involvement in diseaseDefects in OCLN are the cause of band-like calcification with simplified gyration and polymicrogyria (BLCPMG) [MIM:251290]; also known as pseudo-TORCH syndrome. BLCPMG is a neurologic disorder with characteristic clinical and neuroradiologic features that mimic intrauterine TORCH infection in the absence of evidence of infection. Affected individuals have congenital microcephaly, intracranial calcifications, and severe developmental delay.
Sequence similaritiesBelongs to the ELL/occludin family.
Contains 1 MARVEL domain.
DomainThe C-terminal is cytoplasmic and is important for interaction with ZO-1. Sufficient for the tight junction localization. Involved in the regulation of the permeability barrier function of the tight junction (By similarity). The first extracellular loop participates in an adhesive interaction.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. Dephosphorylated by PTPRJ. The tyrosine phosphorylation on Tyr-398 and Tyr-402 reduces its ability to interact with TJP1.
Cellular localizationMembrane. Cell junction > tight junction.
- Information by UniProt
- BLCPMG antibody
- FLJ08163 antibody
- FLJ18079 antibody
Formalin-fixed, paraffin-embedded mouse embryo tissue stained for Occludin using ab222691 at 1/200 dilution in immunohistochemical analysis, followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed, paraffin-embedded mouse kidney tissue stained for Occludin using ab222691 at 1/200 dilution in immunohistochemical analysis, followed by conjugation to the secondary antibody and DAB staining.
Anti-Occludin antibody (ab222691) at 1/300 dilution + A549 cell lysate
Goat Anti-Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated at 1/5000 dilution
Predicted band size: 59 kDa
ab222691 has been referenced in 4 publications.
- Tunisi L et al. Orexin-A Prevents Lipopolysaccharide-Induced Neuroinflammation at the Level of the Intestinal Barrier. Front Endocrinol (Lausanne) 10:219 (2019). PubMed: 31024456
- Liu R et al. Inhibition of lncRNA NEAT1 suppresses the inflammatory response in IBD by modulating the intestinal epithelial barrier and by exosome-mediated polarization of macrophages. Int J Mol Med 42:2903-2913 (2018). PubMed: 30132508
- Gruber S et al. Radioprotective Effects of Dermatan Sulfate in a Preclinical Model of Oral Mucositis-Targeting Inflammation, Hypoxia and Junction Proteins without Stimulating Proliferation. Int J Mol Sci 19:N/A (2018). PubMed: 29882770
- Chen X et al. Protective Role of Coxsackie-Adenovirus Receptor in the Pathogenesis of Inflammatory Bowel Diseases. Biomed Res Int 2018:7207268 (2018). PubMed: 30175139