Key features and details
- Rabbit polyclonal to Occludin
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Occludin antibody
See all Occludin primary antibodies
DescriptionRabbit polyclonal to Occludin
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human Occludin aa 285-430.
Database link: Q16625
- WB: MCF7 whole cell lysate. IHC-P: Human colon cancer tissue. ICC/IF: MCF7 cells.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%
Our Abpromise guarantee covers the use of ab235986 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/50 - 1/200.|
|IHC-P||1/200 - 1/500.|
|WB||1/500 - 1/5000. Predicted molecular weight: 59 kDa.|
FunctionMay play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. It is able to induce adhesion when expressed in cells lacking tight junctions.
Tissue specificityLocalized at tight junctions of both epithelial and endothelial cells. Highly expressed in kidney. Not detected in testis.
Involvement in diseaseDefects in OCLN are the cause of band-like calcification with simplified gyration and polymicrogyria (BLCPMG) [MIM:251290]; also known as pseudo-TORCH syndrome. BLCPMG is a neurologic disorder with characteristic clinical and neuroradiologic features that mimic intrauterine TORCH infection in the absence of evidence of infection. Affected individuals have congenital microcephaly, intracranial calcifications, and severe developmental delay.
Sequence similaritiesBelongs to the ELL/occludin family.
Contains 1 MARVEL domain.
DomainThe C-terminal is cytoplasmic and is important for interaction with ZO-1. Sufficient for the tight junction localization. Involved in the regulation of the permeability barrier function of the tight junction (By similarity). The first extracellular loop participates in an adhesive interaction.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. Dephosphorylated by PTPRJ. The tyrosine phosphorylation on Tyr-398 and Tyr-402 reduces its ability to interact with TJP1.
Cellular localizationMembrane. Cell junction > tight junction.
- Information by UniProt
- BLCPMG antibody
- FLJ08163 antibody
- FLJ18079 antibody
Anti-Occludin antibody (ab235986) at 1/500 dilution + MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 59 kDa
Paraffin-embedded human colon cancer tissue stained for Occludin using ab235986 at 1/400 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.
4% formaldehyde-fixed, 0.2% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells stained for Occludin (green) using ab235986 at 1/133 dilution in ICC/IF, followed by an Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L). Counterstained with DAPI.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab235986 has not yet been referenced specifically in any publications.