Anti-Occludin antibody [CL1555] (ab242202)
Key features and details
- Mouse monoclonal [CL1555] to Occludin
- Suitable for: ICC, IHC-P, WB
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-Occludin antibody [CL1555]
See all Occludin primary antibodies -
Description
Mouse monoclonal [CL1555] to Occludin -
Host species
Mouse -
Tested Applications & Species
Application Species ICC HumanIHC-P HumanWB Human -
Immunogen
Recombinant fragment corresponding to Human Occludin aa 250-450.
Database link: Q16625 -
Epitope
Binds to an epitope located within the peptide sequence TSPVDDFRQPRYSSG as determined by overlapping synthetic peptides. -
Positive control
- WB: Caco-2 cell lysate. ICC: Caco-2 cells. IHC-P: Human stomach, kidney, colon, fallopian tube, liver, and prostate cancer tissue..
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
CL1555 -
Isotype
IgG2a -
Research areas
Associated products
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Compatible Secondaries
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab242202 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
---|---|
ICC |
Human
|
IHC-P |
Human
|
WB |
Human
|
All applications |
Dog
Orangutan
|
Application | Abreviews | Notes |
---|---|---|
ICC |
Use a concentration of 2 - 10 µg/ml.
Fixation: PFA/Triton X-100. |
|
IHC-P |
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 59 kDa.
|
Notes |
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ICC
Use a concentration of 2 - 10 µg/ml. Fixation: PFA/Triton X-100. |
IHC-P
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 59 kDa. |
Target
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Function
May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. It is able to induce adhesion when expressed in cells lacking tight junctions. -
Tissue specificity
Localized at tight junctions of both epithelial and endothelial cells. Highly expressed in kidney. Not detected in testis. -
Involvement in disease
Defects in OCLN are the cause of band-like calcification with simplified gyration and polymicrogyria (BLCPMG) [MIM:251290]; also known as pseudo-TORCH syndrome. BLCPMG is a neurologic disorder with characteristic clinical and neuroradiologic features that mimic intrauterine TORCH infection in the absence of evidence of infection. Affected individuals have congenital microcephaly, intracranial calcifications, and severe developmental delay. -
Sequence similarities
Belongs to the ELL/occludin family.
Contains 1 MARVEL domain. -
Domain
The C-terminal is cytoplasmic and is important for interaction with ZO-1. Sufficient for the tight junction localization. Involved in the regulation of the permeability barrier function of the tight junction (By similarity). The first extracellular loop participates in an adhesive interaction. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. Dephosphorylated by PTPRJ. The tyrosine phosphorylation on Tyr-398 and Tyr-402 reduces its ability to interact with TJP1. -
Cellular localization
Membrane. Cell junction > tight junction. - Information by UniProt
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Database links
- Entrez Gene: 403844 Dog
- Entrez Gene: 100506658 Human
- Entrez Gene: 100174190 Orangutan
- Omim: 602876 Human
- SwissProt: Q28269 Dog
- SwissProt: Q16625 Human
- SwissProt: Q5RFS5 Orangutan
- Unigene: 592605 Human
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Alternative names
- BLCPMG antibody
- FLJ08163 antibody
- FLJ18079 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Occludin antibody [CL1555] (ab242202)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach tissue labelling Occludin with ab242202 at 1/1000 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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PFA-fixed, Triton X-100 permeabilized CACO-2 cells stained for OCLN (Green) using ab242202 at 4 μg/ml in ICC/IF.
Microtublues are stained red, nuclei are stained blue.
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All lanes : Anti-Occludin antibody [CL1555] (ab242202) at 1 µg/ml
Lane 1 : Caco-2 (Human colorectal adenocarcinoma cell line) cell lysate
Lane 2 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cell lysate
Predicted band size: 59 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Occludin antibody [CL1555] (ab242202)
Formalin-fixed, paraffin-embedded human colon tissue stained for Occludin with ab242202 at a 1/500 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Occludin antibody [CL1555] (ab242202)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human fallopian tube tissue labelling Occludin with ab242202 at 1/1000 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Occludin antibody [CL1555] (ab242202)
Formalin-fixed, paraffin-embedded human prostate cancer tissue stained for Occludin with ab242202 at a 1/500 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Occludin antibody [CL1555] (ab242202)
Formalin-fixed, paraffin-embedded human kidney tissue stained for Occludin with ab242202 at a 1/500 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Occludin antibody [CL1555] (ab242202)
Formalin-fixed, paraffin-embedded human liver tissue stained for Occludin with ab242202 at a 1/500 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Occludin antibody [CL1555] (ab242202)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skeeltal muscle tissue labelling Occludin with ab242202 at 1/1000 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab242202 has not yet been referenced specifically in any publications.