Overview

  • Product name

    Anti-Occludin antibody [EPR8208]
    See all Occludin primary antibodies
  • Description

    Rabbit monoclonal [EPR8208] to Occludin
  • Host species

    Rabbit
  • Specificity

    The antibody does not recognize human Occludin isoform 4 and isoform 5 based on the immunogen sequence.
  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Occludin aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: Q16625

  • Positive control

    • Human fetal kidney and HepG2 whole cell lysate (ab7900).
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR8208
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab167161 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50000 - 1/200000. Predicted molecular weight: 59 kDa.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • Target

    • Function

      May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. It is able to induce adhesion when expressed in cells lacking tight junctions.
    • Tissue specificity

      Localized at tight junctions of both epithelial and endothelial cells. Highly expressed in kidney. Not detected in testis.
    • Involvement in disease

      Defects in OCLN are the cause of band-like calcification with simplified gyration and polymicrogyria (BLCPMG) [MIM:251290]; also known as pseudo-TORCH syndrome. BLCPMG is a neurologic disorder with characteristic clinical and neuroradiologic features that mimic intrauterine TORCH infection in the absence of evidence of infection. Affected individuals have congenital microcephaly, intracranial calcifications, and severe developmental delay.
    • Sequence similarities

      Belongs to the ELL/occludin family.
      Contains 1 MARVEL domain.
    • Domain

      The C-terminal is cytoplasmic and is important for interaction with ZO-1. Sufficient for the tight junction localization. Involved in the regulation of the permeability barrier function of the tight junction (By similarity). The first extracellular loop participates in an adhesive interaction.
    • Post-translational
      modifications

      Phosphorylated upon DNA damage, probably by ATM or ATR. Dephosphorylated by PTPRJ. The tyrosine phosphorylation on Tyr-398 and Tyr-402 reduces its ability to interact with TJP1.
    • Cellular localization

      Membrane. Cell junction > tight junction.
    • Information by UniProt
    • Database links

    • Alternative names

      • BLCPMG antibody
      • FLJ08163 antibody
      • FLJ18079 antibody
      • FLJ77961 antibody
      • FLJ94056 antibody
      • MGC34277 antibody
      • Occludin antibody
      • Ocln antibody
      • OCLN_HUMAN antibody
      • Phosphatase 1 regulatory subunit 115 antibody
      • PPP1R115 antibody
      • PTORCH1 antibody
      • Tight junction protein occludin antibody
      see all

    Images

    • All lanes : Anti-Occludin antibody [EPR8208] (ab167161) at 1/50000 dilution

      Lane 1 : Human fetal kidney cell lysate
      Lane 2 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 59 kDa

    • Lane 1 : Anti-Occludin antibody [EPR8208] (ab167161) at 1/5000 dilution
      Lane 2 : Anti-Occludin antibody (ab31721) at 1/1000 dilution
      Lane 3 : Anti-Occludin antibody [EPR20992] (ab216327) at 1/5000 dilution

      All lanes : Recombinant Human Occludin protein (ab114189)

      Lysates/proteins at 0.025 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 59 kDa
      Observed band size: 85 kDa
      why is the actual band size different from the predicted?



      Blocking and diluting buffer: 5% NFDM/TBST

      Exposure time:
      5.5 seconds

    References

    This product has been referenced in:

    • Feng G  et al. Protective effect of biogenic polyphosphate nanoparticles from Synechococcus sp. PCC 7002 on dextran sodium sulphate-induced colitis in mice. Food Funct 10:1007-1016 (2019). Read more (PubMed: 30706920) »
    • Chen L  et al. Unilateral ureteral obstruction causes gut microbial dysbiosis and metabolome disorders contributing to tubulointerstitial fibrosis. Exp Mol Med 51:38 (2019). Read more (PubMed: 30918245) »
    See all 35 Publications for this product

    Customer reviews and Q&As

    1-8 of 8 Abreviews or Q&A

    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Mouse lung tissue)
    Permeabilization
    Yes - 0.1% Triton X
    Specification
    Mouse lung tissue
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 24°C
    Fixative
    Paraformaldehyde

    Dr. Jim Hsiao

    Verified customer

    Submitted Sep 14 2017

    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Primary mouse lung endothelial cells)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    40 µg
    Specification
    Primary mouse lung endothelial cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

    Dr. Jim Hsiao

    Verified customer

    Submitted Sep 13 2017

    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (Human Lung)
    Permeabilization
    Yes - 0.1% Triton X
    Specification
    Human Lung
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 24°C
    Fixative
    Formaldehyde

    Dr. Jim Hsiao

    Verified customer

    Submitted Sep 13 2017

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Nasal epithelial cells)
    Gel Running Conditions
    Non-reduced Denaturing (10%)
    Loading amount
    20 µg
    Treatment
    Rhinovirus
    Specification
    Nasal epithelial cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

    Dr. Kyeongah Kim

    Verified customer

    Submitted Jun 16 2017

    Application
    IHC - Wholemount
    Sample
    Rat Tissue (Trachea)
    Specification
    Trachea

    Abcam user community

    Verified customer

    Submitted Jan 10 2017

    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Intestinal tissue)
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis Tris MOPS)
    Loading amount
    28 µg
    Specification
    Intestinal tissue
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Mr. Julien Chevalier

    Verified customer

    Submitted Jun 04 2015

    Application
    Western blot
    Sample
    Human Tissue lysate - whole (Intestinal tissue (Mucosa))
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis Tris MOPS)
    Loading amount
    28 µg
    Specification
    Intestinal tissue (Mucosa)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Mr. Julien Chevalier

    Verified customer

    Submitted Jun 01 2015

    Answer

    We do not offer a conjugated form of our anti-Occludin antibody at this time and would recommend the use of a secondary antibody against rabbit IgG in Flow Cytometry. If you could tell me which fluorochromes you are interested in using, I would be happy to find an appropriate secondary antibody fro use with this product.

    Read More

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