Product nameAnti-Occludin antibody [EPR8208]
See all Occludin primary antibodies
DescriptionRabbit monoclonal [EPR8208] to Occludin
SpecificityThe antibody does not recognize human Occludin isoform 4 and isoform 5 based on the immunogen sequence.
Tested applicationsSuitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human Occludin aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: Q16625
- Human fetal kidney and HepG2 whole cell lysate (ab7900).
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab167161 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/50000 - 1/200000. Predicted molecular weight: 59 kDa.|
|IP||1/10 - 1/100.|
FunctionMay play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. It is able to induce adhesion when expressed in cells lacking tight junctions.
Tissue specificityLocalized at tight junctions of both epithelial and endothelial cells. Highly expressed in kidney. Not detected in testis.
Involvement in diseaseDefects in OCLN are the cause of band-like calcification with simplified gyration and polymicrogyria (BLCPMG) [MIM:251290]; also known as pseudo-TORCH syndrome. BLCPMG is a neurologic disorder with characteristic clinical and neuroradiologic features that mimic intrauterine TORCH infection in the absence of evidence of infection. Affected individuals have congenital microcephaly, intracranial calcifications, and severe developmental delay.
Sequence similaritiesBelongs to the ELL/occludin family.
Contains 1 MARVEL domain.
DomainThe C-terminal is cytoplasmic and is important for interaction with ZO-1. Sufficient for the tight junction localization. Involved in the regulation of the permeability barrier function of the tight junction (By similarity). The first extracellular loop participates in an adhesive interaction.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. Dephosphorylated by PTPRJ. The tyrosine phosphorylation on Tyr-398 and Tyr-402 reduces its ability to interact with TJP1.
Cellular localizationMembrane. Cell junction > tight junction.
- Information by UniProt
- BLCPMG antibody
- FLJ08163 antibody
- FLJ18079 antibody
All lanes : Anti-Occludin antibody [EPR8208] (ab167161) at 1/50000 dilution
Lane 1 : Human fetal kidney cell lysate
Lane 2 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 59 kDa
Lane 1 : Anti-Occludin antibody [EPR8208] (ab167161) at 1/5000 dilution
Lane 2 : Anti-Occludin antibody (ab31721) at 1/1000 dilution
Lane 3 : Anti-Occludin antibody [EPR20992] (ab216327) at 1/5000 dilution
All lanes :
Recombinant Human Occludin protein (ab114189)
Lysates/proteins at 0.025 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 59 kDa
Observed band size: 85 kDa why is the actual band size different from the predicted?
Blocking and diluting buffer: 5% NFDM/TBST
Exposure time: 5.5 seconds
This product has been referenced in:
- Feng G et al. Protective effect of biogenic polyphosphate nanoparticles from Synechococcus sp. PCC 7002 on dextran sodium sulphate-induced colitis in mice. Food Funct 10:1007-1016 (2019). Read more (PubMed: 30706920) »
- Chen L et al. Unilateral ureteral obstruction causes gut microbial dysbiosis and metabolome disorders contributing to tubulointerstitial fibrosis. Exp Mol Med 51:38 (2019). Read more (PubMed: 30918245) »