Recombinant Anti-Occludin antibody [EPR8208] - BSA and Azide free (ab240150)


  • Product name

    Anti-Occludin antibody [EPR8208] - BSA and Azide free
    See all Occludin primary antibodies
  • Description

    Rabbit monoclonal [EPR8208] to Occludin - BSA and Azide free
  • Host species

  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Occludin aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: Q16625

  • General notes

    Ab240150 is the carrier-free version of ab167161. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.


    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab240150 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.


  • Form

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: PBS
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

  • Clone number

  • Isotype

  • Research areas


Our Abpromise guarantee covers the use of ab240150 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 59 kDa.
IP Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • Target

    • Function

      May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. It is able to induce adhesion when expressed in cells lacking tight junctions.
    • Tissue specificity

      Localized at tight junctions of both epithelial and endothelial cells. Highly expressed in kidney. Not detected in testis.
    • Involvement in disease

      Defects in OCLN are the cause of band-like calcification with simplified gyration and polymicrogyria (BLCPMG) [MIM:251290]; also known as pseudo-TORCH syndrome. BLCPMG is a neurologic disorder with characteristic clinical and neuroradiologic features that mimic intrauterine TORCH infection in the absence of evidence of infection. Affected individuals have congenital microcephaly, intracranial calcifications, and severe developmental delay.
    • Sequence similarities

      Belongs to the ELL/occludin family.
      Contains 1 MARVEL domain.
    • Domain

      The C-terminal is cytoplasmic and is important for interaction with ZO-1. Sufficient for the tight junction localization. Involved in the regulation of the permeability barrier function of the tight junction (By similarity). The first extracellular loop participates in an adhesive interaction.
    • Post-translational

      Phosphorylated upon DNA damage, probably by ATM or ATR. Dephosphorylated by PTPRJ. The tyrosine phosphorylation on Tyr-398 and Tyr-402 reduces its ability to interact with TJP1.
    • Cellular localization

      Membrane. Cell junction > tight junction.
    • Information by UniProt
    • Database links

    • Alternative names

      • BLCPMG antibody
      • FLJ08163 antibody
      • FLJ18079 antibody
      • FLJ77961 antibody
      • FLJ94056 antibody
      • MGC34277 antibody
      • Occludin antibody
      • Ocln antibody
      • OCLN_HUMAN antibody
      • Phosphatase 1 regulatory subunit 115 antibody
      • PPP1R115 antibody
      • PTORCH1 antibody
      • Tight junction protein occludin antibody
      see all


    ab240150 has not yet been referenced specifically in any publications.

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