Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8208] to Occludin - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Product nameAnti-Occludin antibody [EPR8208] - BSA and Azide free
See all Occludin primary antibodies
DescriptionRabbit monoclonal [EPR8208] to Occludin - BSA and Azide free
Tested applicationsSuitable for: WBmore details
Unsuitable for: IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human Occludin aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: Q16625
- WB: Human fetal kidney tissue lysate. HepG2, HUVEC, Rat-1, and bEnd.3 whole cell lysates. Mouse and rat spinal cord and small intestine tissue lysates.
Ab240150 is the carrier-free version of ab167161. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab240150 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab240150 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 59 kDa.|
FunctionMay play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. It is able to induce adhesion when expressed in cells lacking tight junctions.
Tissue specificityLocalized at tight junctions of both epithelial and endothelial cells. Highly expressed in kidney. Not detected in testis.
Involvement in diseaseDefects in OCLN are the cause of band-like calcification with simplified gyration and polymicrogyria (BLCPMG) [MIM:251290]; also known as pseudo-TORCH syndrome. BLCPMG is a neurologic disorder with characteristic clinical and neuroradiologic features that mimic intrauterine TORCH infection in the absence of evidence of infection. Affected individuals have congenital microcephaly, intracranial calcifications, and severe developmental delay.
Sequence similaritiesBelongs to the ELL/occludin family.
Contains 1 MARVEL domain.
DomainThe C-terminal is cytoplasmic and is important for interaction with ZO-1. Sufficient for the tight junction localization. Involved in the regulation of the permeability barrier function of the tight junction (By similarity). The first extracellular loop participates in an adhesive interaction.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. Dephosphorylated by PTPRJ. The tyrosine phosphorylation on Tyr-398 and Tyr-402 reduces its ability to interact with TJP1.
Cellular localizationMembrane. Cell junction > tight junction.
- Information by UniProt
- BLCPMG antibody
- FLJ08163 antibody
- FLJ18079 antibody
All lanes : Anti-Occludin antibody [EPR8208] (ab167161) at 1/1000 dilution (Purified)
Lane 1 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate
Lane 2 : HUVEC (Human umbilical vein endothelial cell) whole cell lysate
Lane 3 : bEnd.3 (Mouse brain endothelioma) whole cell lysate
Lane 4 : Mouse brain lysate
Lane 5 : C57 mouse spinal cord lysate
Lane 6 : Mouse small intestine lysate
Lane 8 : Rat spinal cord lysate
Lane 9 : Rat small intestine lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 59 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted?
The molecular weight observed is consistent as what is described in PMID: 17332770, PMID: 24884662 and PMID: 15054114.
Although some papers support the expression of Occludin in HUVEC (PMID:31057027, 29927318) and bEnd.3 (PMID: 31096970), ab167161 cannot detect band of interest in these cell lines.
The expression of occludin in brain, spinal cord and intestine is consistent with what has been described in PMID: 31511021, PMID: 30742658 and PMID: 29927318.
Blocking/Diluting buffer: 5% NFDM/TBST
Loading Control: Rabbit monoclonal [EPR16891] to GAPDH (ab181602)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab167161).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab240150 has not yet been referenced specifically in any publications.