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    occludin-antibody-neural-stem-cell-marker-ab168986.pdf

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Signal Transduction Cytoskeleton / ECM Cell Adhesion Tight Junctions
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Anti-Occludin antibody - Neural Stem Cell Marker (ab168986)

  • Datasheet
  • SDS
Reviews (4) Submit a question References (20)

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Western blot - Anti-Occludin antibody - Neural Stem Cell Marker (ab168986)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Occludin antibody - Neural Stem Cell Marker (ab168986)
  • Western blot - Anti-Occludin antibody - Neural Stem Cell Marker (ab168986)
  • Western blot - Anti-Occludin antibody - Neural Stem Cell Marker (ab168986)

Key features and details

  • Rabbit polyclonal to Occludin - Neural Stem Cell Marker
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG

You may also be interested in

Protein
Product image
Recombinant Human Occludin protein (ab114189)
Primary
Product image
Anti-Junctional Adhesion Molecule 2/JAM-B antibody (ab139645)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-Occludin antibody - Neural Stem Cell Marker
    See all Occludin primary antibodies
  • Description

    Rabbit polyclonal to Occludin - Neural Stem Cell Marker
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Mouse
    Human
    See all applications and species data
  • Immunogen

    Recombinant full length protein corresponding to Human Occludin aa 1-522.
    Sequence:

    MSSRPLESPPPYRPDEFKPNHYAPSNDIYGGEMHVRPMLSQPAYSFYPED EILHFYKWTSPPGVIRILSMLIIVMCIAIFACVASTLAWDRGYGTSLLGG SVGYPYGGSGFGSYGSGYGYGYGYGYGYGGYTDPRAAKGFMLAMAAFCFI AALVIFVTSVIRSEMSRTRRYYLSVIIVSAILGIMVFIATIVYIMGVNPT AQSSGSLYGSQIYALCNQFYTPAATGLYVDQYLYHYCVVDPQEAIAIVLG FMIIVAFALIIFFAVKTRRKMDRYDKSNILWDKEHIYDEQPPNVEEWVKN VSAGTQDVPSPPSDYVERVDSPMAYSSNGKVNDKRFYPESSYKSTPVPEV VQELPLTSPVDDFRQPRYSSGGNFETPSKRAPAKGRAGRSKRTEQDHYET DYTTGGESCDELEEDWIREYPPITSDQQRQLYKRNFDTGLQEYKSLQSEL DEINKELSRLDKELDDYREESEEYMAAADEYNRLKQVKGSADYKSKKNHC KQLKSKLSHIKKMVGDYDRQKT


    Database link: NP_002529.1
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • Human and Mouse liver lysates; Occludin-transfected 293T cell line lysate This antibody gave a positive result in IHC in the following FFPE tissue: Human normal kidney

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.4
    Constituent: 100% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Tight Junctions

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Mouse liver tissue lysate - total protein (ab29301)
  • Recombinant Protein

    • Recombinant Human Occludin protein (ab114189)
  • Related Products

    • Recombinant Human Occludin protein (ab114189)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab168986 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
WB
Mouse
Human
All applications
Rat
Dog
Orangutan
Application Abreviews Notes
WB (2)
Use a concentration of 1 µg/ml. Predicted molecular weight: 59 kDa.
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 59 kDa.
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. It is able to induce adhesion when expressed in cells lacking tight junctions.
  • Tissue specificity

    Localized at tight junctions of both epithelial and endothelial cells. Highly expressed in kidney. Not detected in testis.
  • Involvement in disease

    Defects in OCLN are the cause of band-like calcification with simplified gyration and polymicrogyria (BLCPMG) [MIM:251290]; also known as pseudo-TORCH syndrome. BLCPMG is a neurologic disorder with characteristic clinical and neuroradiologic features that mimic intrauterine TORCH infection in the absence of evidence of infection. Affected individuals have congenital microcephaly, intracranial calcifications, and severe developmental delay.
  • Sequence similarities

    Belongs to the ELL/occludin family.
    Contains 1 MARVEL domain.
  • Domain

    The C-terminal is cytoplasmic and is important for interaction with ZO-1. Sufficient for the tight junction localization. Involved in the regulation of the permeability barrier function of the tight junction (By similarity). The first extracellular loop participates in an adhesive interaction.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR. Dephosphorylated by PTPRJ. The tyrosine phosphorylation on Tyr-398 and Tyr-402 reduces its ability to interact with TJP1.
  • Cellular localization

    Membrane. Cell junction > tight junction.
  • Target information above from: UniProt accession Q16625 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 403844 Dog
    • Entrez Gene: 100506658 Human
    • Entrez Gene: 18260 Mouse
    • Entrez Gene: 100174190 Orangutan
    • Entrez Gene: 83497 Rat
    • Omim: 602876 Human
    • SwissProt: Q28269 Dog
    • SwissProt: Q16625 Human
    • SwissProt: Q61146 Mouse
    • SwissProt: Q5RFS5 Orangutan
    • SwissProt: Q6P6T5 Rat
    • Unigene: 592605 Human
    • Unigene: 4807 Mouse
    • Unigene: 31429 Rat
    see all
  • Alternative names

    • BLCPMG antibody
    • FLJ08163 antibody
    • FLJ18079 antibody
    • FLJ77961 antibody
    • FLJ94056 antibody
    • MGC34277 antibody
    • Occludin antibody
    • Ocln antibody
    • OCLN_HUMAN antibody
    • Phosphatase 1 regulatory subunit 115 antibody
    • PPP1R115 antibody
    • PTORCH1 antibody
    • Tight junction protein occludin antibody
    see all

Images

  • Western blot - Anti-Occludin antibody - Neural Stem Cell Marker (ab168986)
    Western blot - Anti-Occludin antibody - Neural Stem Cell Marker (ab168986)
    Anti-Occludin antibody - Neural Stem Cell Marker (ab168986) at 1 µg/ml + Human liver lysate at 50 µg

    Secondary
    Goat Anti-Rabbit IgG (H+L)-HRP at 1/7500 dilution

    Developed using the ECL technique.

    Predicted band size: 59 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Occludin antibody - Neural Stem Cell Marker (ab168986)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Occludin antibody - Neural Stem Cell Marker (ab168986)

    IHC image of Occludin staining in Human normal kidney formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab168986, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Western blot - Anti-Occludin antibody - Neural Stem Cell Marker (ab168986)
    Western blot - Anti-Occludin antibody - Neural Stem Cell Marker (ab168986)
    Anti-Occludin antibody - Neural Stem Cell Marker (ab168986) at 1 µg/ml + Mouse liver lysate at 50 µg

    Secondary
    Goat Anti-Rabbit IgG (H+L)-HRP at 1/7500 dilution

    Developed using the ECL technique.

    Predicted band size: 59 kDa

  • Western blot - Anti-Occludin antibody - Neural Stem Cell Marker (ab168986)
    Western blot - Anti-Occludin antibody - Neural Stem Cell Marker (ab168986)
    All lanes : Anti-Occludin antibody - Neural Stem Cell Marker (ab168986) at 1 µg/ml

    Lane 1 : Occludin-transfected 293T cell line lysate
    Lane 2 : Non-transfected 293T cell line lysate

    Secondary
    All lanes : Goat Anti-Rabbit IgG at 1/7500 dilution

    Developed using the ECL technique.

    Predicted band size: 59 kDa

Protocols

  • Western blot protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (20)

    Publishing research using ab168986? Please let us know so that we can cite the reference in this datasheet.

    ab168986 has been referenced in 20 publications.

    • Hu X  et al. The RNA-binding protein AKAP8 suppresses tumor metastasis by antagonizing EMT-associated alternative splicing. Nat Commun 11:486 (2020). PubMed: 31980632
    • Li JM  et al. Dietary fructose-induced gut dysbiosis promotes mouse hippocampal neuroinflammation: a benefit of short-chain fatty acids. Microbiome 7:98 (2019). PubMed: 31255176
    • Wu Y  et al. Silencing of RAD51AP1 suppresses epithelial-mesenchymal transition and metastasis in non-small cell lung cancer. Thorac Cancer 10:1748-1763 (2019). PubMed: 31317661
    • Xiang Y  et al. USP9X promotes LPS-induced pulmonary epithelial barrier breakdown and hyperpermeability by activating an NF-?Bp65 feedback loop. Am J Physiol Cell Physiol 317:C534-C543 (2019). PubMed: 31216195
    • Dong JP  et al. Protective effect of Saccharomyces boulardii on intestinal mucosal barrier of dextran sodium sulfate-induced colitis in mice. Chin Med J (Engl) 132:1951-1958 (2019). PubMed: 31335471
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-4 of 4 Abreviews

    Western blot abreview for Anti-Occludin antibody - Neural Stem Cell Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Cell lysate - whole cell (Enteric glial cell)
    Gel Running Conditions
    Non-reduced Denaturing (4-12% Bis Acrylamide)
    Loading amount
    50 µg
    Specification
    Enteric glial cell
    Blocking step
    Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 08 2017

    Immunohistochemistry (Frozen sections) abreview for Anti-Occludin antibody - Neural Stem Cell Marker

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Rat Tissue sections (brain)
    Permeabilization
    Yes - Triton-X 100
    Specification
    brain
    Blocking step
    donkey serum as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
    Fixative
    Paraformaldehyde
    Read More

    Alyssa Cortella

    Verified customer

    Submitted Mar 14 2017

    Western blot abreview for Anti-Occludin antibody - Neural Stem Cell Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rhesus monkey Tissue lysate - whole (Colon)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    2500 µg
    Specification
    Colon
    Blocking step
    BSA as blocking agent for 45 minute(s) · Concentration: 2% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 09 2016

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Occludin antibody - Neural Stem Cell Marker

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (CaCo-2)
    Permeabilization
    Yes - 0.5% Triton X-100
    Specification
    CaCo-2
    Blocking step
    BSA as blocking agent for 30 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More

    Mrs. Annelies Bunschoten

    Verified customer

    Submitted Nov 01 2016

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