Overview

  • Product name
    Anti-Ochratoxin A antibody [3C5]
    See all Ochratoxin A primary antibodies
  • Description
    Mouse monoclonal [3C5] to Ochratoxin A
  • Host species
    Mouse
  • Tested applications
    Suitable for: ELISAmore details
  • Immunogen

    Chemical/ Small Molecule corresponding to Ochratoxin A conjugated to Bovine Serum Albumin (BSA).

  • Positive control
    • Ochratoxin A from Sigma ( reference O1877)

Properties

Applications

Our Abpromise guarantee covers the use of ab23965 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/2000 - 1/10000.

Target

  • Relevance
    Ochratoxin A is a polyketide-derived dihydroiso-coumarin moiety linked through the 12-carboxy group to phenylalanine. Ochratoxin A is the most important and most commonly occurring of a structurally related group of compounds. It is produced by some species of Aspergillus, such as A. ochraceus, mainly in tropical regions, and by Penicillium verrucosum, a common storage fungus in temperate areas such as Canada, eastern and north western Europe and parts of South America. Ochratoxin A is a potent toxin affecting mainly the kidneys, in which it can cause both acute and chronic lesions, whereas its dechloro derivative, ochratoxin B, is non-toxic. A nephrotoxic effect has been demonstrated in all mammalian species.
  • Cellular localization
    Secreted
  • Alternative names
    • OchratoxinA antibody

References

ab23965 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Question
Answer

The answer is Yes, the antibody ab23965 will recognize Ochratoxin A when it would be coupled to HRP.

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Answer

The antibody ab23965 has been tested on Ochratoxin A from Sigma, Ref. O 1877. http://www.sigmaaldrich.com/catalog/search/ProductDetail/SIGMA/O1877 The following protocol was used: Ochratoxin A is coupled on BSA and used as antigen. -This antigen is coated in a 96 wells plate (EIA/RIA high binding Ref. Corning Costar 3590) at ~ 10µg/ml in bicabonate buffer pH 9.5. Incubation 1 hour at 37°C with shaking. - Saturation : coupling solution is eliminated by turning over the plate. Add 150 µl of BSA 0.5% in PBS. Incubation 1/2 hour at 37°C with shaking. - Eliminate the BSA solution. Add 0.1ml of ab23965 diluted at 1/5000 in PBS/Tween 20(0.1 %). Incubation 1/2 hour to 1 hour at 37°C with shaking. - Wash the plate 4 time with PBS/Tween 20 (0.1%) or TBS/Tween 20 (0.1%). Add 0.1ml of diluted secondary antibody. Incubation 1/2 hour at 37°C with shaking. - Wash the plate 4 time with PBS/Tween 20 (0.1%) or TBS/Tween 20 (0.1%). Add 0.1ml Chromogen. (to be chosen according the enzyme used for labelling the secondary antibody). -Stop the reaction. -Read the OD of the wells. I will add this information to the datasheet and hope it will be useful to you,

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Answer

Thank you for your enquiry. This antibody has not been epitope-mapped, so the epitope could reside anywhere along the length of the protein. Therefore, it is impossible to say whether the epitope of this antibody overlaps with the carboxylic group. I am sorry I couldn't be more helpful. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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Question
Answer

Thank you for your enquiry. I have enquired at the originator of the product but unfortunately, the coupling to BSA method is confidential and cannot be released. I am sorry I can't be more helpful in this occasion. Should you require anything else, please do not hesitate to contact me.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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