Anti-OCRL antibody (ab125917)
Key features and details
- Rabbit polyclonal to OCRL
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-OCRL antibody
See all OCRL primary antibodies -
Description
Rabbit polyclonal to OCRL -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human OCRL aa 591-857.
Database link: Q01968 -
Positive control
- 293T and A431 cell lysates, A431 cells and Human Breast ca tissue.
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General notes
This product was previously labelled as INPP5F
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab125917 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Detects a band of approximately 104 kDa. | |
| IHC-P | 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. citrate buffer-10mM | |
| ICC/IF | 1/100 - 1/500. |
Target
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Function
Converts phosphatidylinositol 4,5-bisphosphate to phosphatidylinositol 4-phosphate. Also converts inositol 1,4,5-trisphosphate to inositol 1,4-bisphosphate and inositol 1,3,4,5-tetrakisphosphate to inositol 1,3,4-trisphosphate. May function in lysosomal membrane trafficking by regulating the specific pool of phosphatidylinositol 4,5-bisphosphate that is associated with lysosomes. -
Tissue specificity
Brain, skeletal muscle, heart, kidney, lung, placenta and fibroblasts. -
Involvement in disease
Defects in OCRL are the cause of Lowe oculocerebrorenal syndrome (OCRL) [MIM:309000]. It is an X-linked multisystem disorder affecting eyes, nervous system, and kidney. It is characterized by hydrophthalmia, cataract, mental retardation, vitamin D-resistant rickets, aminoaciduria, and reduced ammonia production by the kidney. Ocular abnormalities include cataract, glaucoma, microphthalmos, and decreased visual acuity. Developmental delay, hypotonia, behavior abnormalities, and areflexia are also present. Renal tubular involvement is characterized by impaired reabsorption of bicarbonate, amino acids, and phosphate. Musculoskeletal abnormalities such as joint hypermobility, dislocated hips, and fractures may develop as consequences of renal tubular acidosis and hypophosphatemia. Cataract is the only significant manifestation in carriers and is detected by slit-lamp examination.
Defects in OCRL are the cause of Dent disease type 2 (DD2) [MIM:300555]. DD2 is a renal disease belonging to the 'Dent disease complex', a group of disorders characterized by proximal renal tubular defect, hypercalciuria, nephrocalcinosis, and renal insufficiency. The spectrum of phenotypic features is remarkably similar in the various disorders, except for differences in the severity of bone deformities and renal impairment. Characteristic abnormalities include low-molecular-weight proteinuria and other features of Fanconi syndrome, such as glycosuria, aminoaciduria, and phosphaturia, but typically do not include proximal renal tubular acidosis. Progressive renal failure is common, as are nephrocalcinosis and kidney stones. -
Sequence similarities
Belongs to the inositol-1,4,5-trisphosphate 5-phosphatase type II family.
Contains 1 Rho-GAP domain. -
Cellular localization
Endosome. Also found on macropinosomes. - Information by UniProt
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Database links
- Entrez Gene: 4952 Human
- Omim: 300535 Human
- SwissProt: Q01968 Human
- Unigene: 126357 Human
- Unigene: 369755 Human
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Alternative names
- EC 3.1.3.36 antibody
- Inositol polyphosphate 5 phosphatase OCRL 1 antibody
- Inositol polyphosphate 5 phosphatase OCRL1 antibody
see all
Images
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Western blot - Anti-OCRL antibody (ab125917)All lanes : Anti-OCRL antibody (ab125917) at 1/500 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lysates/proteins at 30 µg per lane.
Gel concentration:5% -
Immunocytochemistry/ Immunofluorescence - Anti-OCRL antibody (ab125917)ab125917, at 1/500, staining OCRL in Human A431 cells (methanol-fixed) by immunofluorescence (panel 1) and co-stained with Hoechst 33342 (panel 2).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OCRL antibody (ab125917)ab125917, at 1/250, staining OCRL in Human Breast ca tissue by immunohistochemistry [Paraffin Embedded Tissues (IHC-P)].
Protocols
References (0)
ab125917 has not yet been referenced specifically in any publications.
