Validated using a knockout cell lineRabMAb

Anti-OCRL antibody [EP10256] (ab181039)

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Key features and details

  • Rabbit monoclonal [EP10256] to OCRL
  • Suitable for: WB, Flow Cyt
  • Knockout validated
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-OCRL antibody [EP10256]
    See all OCRL primary antibodies

  • Description

    Rabbit monoclonal [EP10256] to OCRL

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC/IF,IHC-P or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human OCRL aa 150-250 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q01968

  • Positive control

    • 293T, SH-SY5Y, HeLa and JAR cell lysates. Permeabilized SH-SY5Y cells.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     This product was previously labelled as INPP5F

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EP10256

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab181039 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 104 kDa.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.

    • Target

    • Function

      Converts phosphatidylinositol 4,5-bisphosphate to phosphatidylinositol 4-phosphate. Also converts inositol 1,4,5-trisphosphate to inositol 1,4-bisphosphate and inositol 1,3,4,5-tetrakisphosphate to inositol 1,3,4-trisphosphate. May function in lysosomal membrane trafficking by regulating the specific pool of phosphatidylinositol 4,5-bisphosphate that is associated with lysosomes.

    • Tissue specificity

      Brain, skeletal muscle, heart, kidney, lung, placenta and fibroblasts.

    • Involvement in disease

      Defects in OCRL are the cause of Lowe oculocerebrorenal syndrome (OCRL) [MIM:309000]. It is an X-linked multisystem disorder affecting eyes, nervous system, and kidney. It is characterized by hydrophthalmia, cataract, mental retardation, vitamin D-resistant rickets, aminoaciduria, and reduced ammonia production by the kidney. Ocular abnormalities include cataract, glaucoma, microphthalmos, and decreased visual acuity. Developmental delay, hypotonia, behavior abnormalities, and areflexia are also present. Renal tubular involvement is characterized by impaired reabsorption of bicarbonate, amino acids, and phosphate. Musculoskeletal abnormalities such as joint hypermobility, dislocated hips, and fractures may develop as consequences of renal tubular acidosis and hypophosphatemia. Cataract is the only significant manifestation in carriers and is detected by slit-lamp examination.
      Defects in OCRL are the cause of Dent disease type 2 (DD2) [MIM:300555]. DD2 is a renal disease belonging to the 'Dent disease complex', a group of disorders characterized by proximal renal tubular defect, hypercalciuria, nephrocalcinosis, and renal insufficiency. The spectrum of phenotypic features is remarkably similar in the various disorders, except for differences in the severity of bone deformities and renal impairment. Characteristic abnormalities include low-molecular-weight proteinuria and other features of Fanconi syndrome, such as glycosuria, aminoaciduria, and phosphaturia, but typically do not include proximal renal tubular acidosis. Progressive renal failure is common, as are nephrocalcinosis and kidney stones.

    • Sequence similarities

      Belongs to the inositol-1,4,5-trisphosphate 5-phosphatase type II family.
      Contains 1 Rho-GAP domain.

    • Cellular localization

      Endosome. Also found on macropinosomes.
    • Information by UniProt

    • Database links

      • Alternative names

        • EC 3.1.3.36 antibody
        • Inositol polyphosphate 5 phosphatase OCRL 1 antibody
        • Inositol polyphosphate 5 phosphatase OCRL1 antibody
        • Inositol polyphosphate 5-phosphatase OCRL-1 antibody
        • INPP5F antibody
        • LOCR antibody
        • Lowe oculocerebrorenal syndrome protein antibody
        • NPHL2 antibody
        • OCRL 1 antibody
        • OCRL antibody
        • OCRL_HUMAN antibody
        • OCRL1 antibody
        • Oculocerebrorenal syndrome of Lowe antibody
        • Phosphatidylinositol polyphosphate 5 phosphatase antibody
        see all

      Images

      • Western blot - Anti-OCRL antibody [EP10256] (ab181039)
        Western blot - Anti-OCRL antibody [EP10256] (ab181039)

        Lane 1: Wild-type HAP1 cell lysate (20 µg)
        Lane 2: INPP5F knockout HAP1 cell lysate (20 µg)
        Lane 3: SH-SY5Y cell lysate (20 µg)
        Lane 4: HeLa cell lysate (20 µg)
        Lanes 1 - 4: Merged signal (red and green). Green - ab181039 observed at 105 kDa. Red - loading control, ab8245, observed at 37 kDa.

        ab181039 was shown to recognize INPP5F when INPP5F knockout samples were used, along with additional cross-reactive bands. Wild-type and INPP5F knockout samples were subjected to SDS-PAGE. ab181039 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

      • Western blot - Anti-OCRL antibody [EP10256] (ab181039)
        Western blot - Anti-OCRL antibody [EP10256] (ab181039)
        All lanes : Anti-OCRL antibody [EP10256] (ab181039) at 1000 µg

        Lane 1 : 293T cell lysates
        Lane 2 : SH-SY5Y cell lysates
        Lane 3 : HeLa cell lysates
        Lane 4 : JAR cell lysates

        Lysates/proteins at 10 µg per lane.

        Predicted band size: 104 kDa

      • Flow Cytometry - Anti-OCRL antibody [EP10256] (ab181039)
        Flow Cytometry - Anti-OCRL antibody [EP10256] (ab181039)

        Flow cytometric analysis of permeabilized SH-SY5Y cells using ab181039 at a 1/10 dilution (red) or a rabbit IgG (negative) (green).

      References (1)

      Publishing research using ab181039? Please let us know so that we can cite the reference in this datasheet.

      ab181039 has been referenced in 1 publication.

      • Daste F  et al. Control of actin polymerization via the coincidence of phosphoinositides and high membrane curvature. J Cell Biol 216:3745-3765 (2017). PubMed: 28923975

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