Overview

  • Product name

    Anti-OCRL antibody [EP10256]
    See all OCRL primary antibodies
  • Description

    Rabbit monoclonal [EP10256] to OCRL
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human OCRL aa 150-250 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q01968

  • Positive control

    • 293T, SH-SY5Y, HeLa and JAR cell lysates. Permeabilized SH-SY5Y cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     This product was previously labelled as INPP5F

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab181039 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 104 kDa.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.
  • Target

    • Function

      Converts phosphatidylinositol 4,5-bisphosphate to phosphatidylinositol 4-phosphate. Also converts inositol 1,4,5-trisphosphate to inositol 1,4-bisphosphate and inositol 1,3,4,5-tetrakisphosphate to inositol 1,3,4-trisphosphate. May function in lysosomal membrane trafficking by regulating the specific pool of phosphatidylinositol 4,5-bisphosphate that is associated with lysosomes.
    • Tissue specificity

      Brain, skeletal muscle, heart, kidney, lung, placenta and fibroblasts.
    • Involvement in disease

      Defects in OCRL are the cause of Lowe oculocerebrorenal syndrome (OCRL) [MIM:309000]. It is an X-linked multisystem disorder affecting eyes, nervous system, and kidney. It is characterized by hydrophthalmia, cataract, mental retardation, vitamin D-resistant rickets, aminoaciduria, and reduced ammonia production by the kidney. Ocular abnormalities include cataract, glaucoma, microphthalmos, and decreased visual acuity. Developmental delay, hypotonia, behavior abnormalities, and areflexia are also present. Renal tubular involvement is characterized by impaired reabsorption of bicarbonate, amino acids, and phosphate. Musculoskeletal abnormalities such as joint hypermobility, dislocated hips, and fractures may develop as consequences of renal tubular acidosis and hypophosphatemia. Cataract is the only significant manifestation in carriers and is detected by slit-lamp examination.
      Defects in OCRL are the cause of Dent disease type 2 (DD2) [MIM:300555]. DD2 is a renal disease belonging to the 'Dent disease complex', a group of disorders characterized by proximal renal tubular defect, hypercalciuria, nephrocalcinosis, and renal insufficiency. The spectrum of phenotypic features is remarkably similar in the various disorders, except for differences in the severity of bone deformities and renal impairment. Characteristic abnormalities include low-molecular-weight proteinuria and other features of Fanconi syndrome, such as glycosuria, aminoaciduria, and phosphaturia, but typically do not include proximal renal tubular acidosis. Progressive renal failure is common, as are nephrocalcinosis and kidney stones.
    • Sequence similarities

      Belongs to the inositol-1,4,5-trisphosphate 5-phosphatase type II family.
      Contains 1 Rho-GAP domain.
    • Cellular localization

      Endosome. Also found on macropinosomes.
    • Information by UniProt
    • Database links

    • Alternative names

      • EC 3.1.3.36 antibody
      • Inositol polyphosphate 5 phosphatase OCRL 1 antibody
      • Inositol polyphosphate 5 phosphatase OCRL1 antibody
      • Inositol polyphosphate 5-phosphatase OCRL-1 antibody
      • INPP5F antibody
      • LOCR antibody
      • Lowe oculocerebrorenal syndrome protein antibody
      • NPHL2 antibody
      • OCRL 1 antibody
      • OCRL antibody
      • OCRL_HUMAN antibody
      • OCRL1 antibody
      • Oculocerebrorenal syndrome of Lowe antibody
      • Phosphatidylinositol polyphosphate 5 phosphatase antibody
      see all

    Images

    • Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: INPP5F knockout HAP1 cell lysate (20 µg)
      Lane 3: SH-SY5Y cell lysate (20 µg)
      Lane 4: HeLa cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab181039 observed at 105 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab181039 was shown to recognize INPP5F when INPP5F knockout samples were used, along with additional cross-reactive bands. Wild-type and INPP5F knockout samples were subjected to SDS-PAGE. ab181039 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • All lanes : Anti-OCRL antibody [EP10256] (ab181039) at 1000 µg

      Lane 1 : 293T cell lysates
      Lane 2 : SH-SY5Y cell lysates
      Lane 3 : HeLa cell lysates
      Lane 4 : JAR cell lysates

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 104 kDa

    • Flow cytometric analysis of permeabilized SH-SY5Y cells using ab181039 at a 1/10 dilution (red) or a rabbit IgG (negative) (green).

    References

    This product has been referenced in:

    • Daste F  et al. Control of actin polymerization via the coincidence of phosphoinositides and high membrane curvature. J Cell Biol 216:3745-3765 (2017). Read more (PubMed: 28923975) »
    See 1 Publication for this product

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