Anti-OCRL antibody [EP10256] (ab181039)
![Western blot - Anti-OCRL antibody [EP10256] (ab181039)](https://www.abcam.com/ps/products/181/ab181039/Images/ab181039-266741-anti-inpp5f-antibody-ep10256-western-blot.jpg "Western blot - Anti-OCRL antibody [EP10256] (ab181039)")
Key features and details
- Rabbit monoclonal [EP10256] to OCRL
- Suitable for: Flow Cyt (Intra), WB
- Knockout validated
- Reacts with: Human
- Isotype: IgG
Related conjugates and formulations
Overview
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Product name
Anti-OCRL antibody [EP10256]
See all OCRL primary antibodies -
Description
Rabbit monoclonal [EP10256] to OCRL -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WBmore details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- 293T, SH-SY5Y, HeLa and JAR cell lysates. Permeabilized SH-SY5Y cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP10256 -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab181039 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| WB |
1/1000 - 1/5000. Predicted molecular weight: 104 kDa.
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| Notes |
|---|
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB
1/1000 - 1/5000. Predicted molecular weight: 104 kDa. |
Target
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Function
Converts phosphatidylinositol 4,5-bisphosphate to phosphatidylinositol 4-phosphate. Also converts inositol 1,4,5-trisphosphate to inositol 1,4-bisphosphate and inositol 1,3,4,5-tetrakisphosphate to inositol 1,3,4-trisphosphate. May function in lysosomal membrane trafficking by regulating the specific pool of phosphatidylinositol 4,5-bisphosphate that is associated with lysosomes. -
Tissue specificity
Brain, skeletal muscle, heart, kidney, lung, placenta and fibroblasts. -
Involvement in disease
Defects in OCRL are the cause of Lowe oculocerebrorenal syndrome (OCRL) [MIM:309000]. It is an X-linked multisystem disorder affecting eyes, nervous system, and kidney. It is characterized by hydrophthalmia, cataract, mental retardation, vitamin D-resistant rickets, aminoaciduria, and reduced ammonia production by the kidney. Ocular abnormalities include cataract, glaucoma, microphthalmos, and decreased visual acuity. Developmental delay, hypotonia, behavior abnormalities, and areflexia are also present. Renal tubular involvement is characterized by impaired reabsorption of bicarbonate, amino acids, and phosphate. Musculoskeletal abnormalities such as joint hypermobility, dislocated hips, and fractures may develop as consequences of renal tubular acidosis and hypophosphatemia. Cataract is the only significant manifestation in carriers and is detected by slit-lamp examination.
Defects in OCRL are the cause of Dent disease type 2 (DD2) [MIM:300555]. DD2 is a renal disease belonging to the 'Dent disease complex', a group of disorders characterized by proximal renal tubular defect, hypercalciuria, nephrocalcinosis, and renal insufficiency. The spectrum of phenotypic features is remarkably similar in the various disorders, except for differences in the severity of bone deformities and renal impairment. Characteristic abnormalities include low-molecular-weight proteinuria and other features of Fanconi syndrome, such as glycosuria, aminoaciduria, and phosphaturia, but typically do not include proximal renal tubular acidosis. Progressive renal failure is common, as are nephrocalcinosis and kidney stones. -
Sequence similarities
Belongs to the inositol-1,4,5-trisphosphate 5-phosphatase type II family.
Contains 1 Rho-GAP domain. -
Cellular localization
Endosome. Also found on macropinosomes. - Information by UniProt
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Database links
- Entrez Gene: 4952 Human
- Omim: 300535 Human
- SwissProt: Q01968 Human
- Unigene: 126357 Human
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Alternative names
- EC 3.1.3.36 antibody
- Inositol polyphosphate 5 phosphatase OCRL 1 antibody
- Inositol polyphosphate 5 phosphatase OCRL1 antibody
see all
Images
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Western blot - Anti-OCRL antibody [EP10256] (ab181039)
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: INPP5F knockout HAP1 cell lysate (20 µg)
Lane 3: SH-SY5Y cell lysate (20 µg)
Lane 4: HeLa cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab181039 observed at 105 kDa. Red - loading control, ab8245, observed at 37 kDa.ab181039 was shown to recognize INPP5F when INPP5F knockout samples were used, along with additional cross-reactive bands. Wild-type and INPP5F knockout samples were subjected to SDS-PAGE. ab181039 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-OCRL antibody [EP10256] (ab181039)](https://www.abcam.com/ps/products/181/ab181039/Images/ab181039-211266-anti-inpp5f-antibody-ep10256-western-blot.jpg)
Western blot - Anti-OCRL antibody [EP10256] (ab181039)All lanes : Anti-OCRL antibody [EP10256] (ab181039) at 1000 µg
Lane 1 : 293T cell lysates
Lane 2 : SH-SY5Y cell lysates
Lane 3 : HeLa cell lysates
Lane 4 : JAR cell lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 104 kDa -
![Flow Cytometry (Intracellular) - Anti-OCRL antibody [EP10256] (ab181039)](https://www.abcam.com/ps/products/181/ab181039/Images/ab181039-211265-anti-inpp5f-antibody-ep10256-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-OCRL antibody [EP10256] (ab181039)Intracellular flow cytometric analysis of permeabilized SH-SY5Y cells using ab181039 at a 1/10 dilution (red) or a rabbit IgG (negative) (green).
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (1)
ab181039 has been referenced in 1 publication.
- Daste F et al. Control of actin polymerization via the coincidence of phosphoinositides and high membrane curvature. J Cell Biol 216:3745-3765 (2017). PubMed: 28923975
