Overview

  • Product name
  • Description
    Rabbit polyclonal to Oct-1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: EMSA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human Oct-1 aa 700 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P14859

  • Positive control
    • Tonsil WB: Jurkat lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.40
    Preservative: 0.1% Sodium azide
    Constituents: 0.0268% PBS, 1% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab15112 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
EMSA Use at an assay dependent concentration. PubMed: 20143233
WB Use at an assay dependent concentration. Detects a band of approximately 80 kDa (predicted molecular weight: 89 kDa).
IHC-P 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function
    Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and activates the promoters of the genes for some small nuclear RNAs (snRNA) and of genes such as those for histone H2B and immunoglobulins. Modulates transcription transactivation by NR3C1, AR and PGR (By similarity). In case of human herpes simplex virus (HSV) infection, POU2F1 forms a multiprotein-DNA complex with the viral transactivator protein VP16 and HCFC1 thereby enabling the transcription of the viral immediate early genes.
  • Tissue specificity
    Ubiquitous. Isoform 2 is lymphocyte-specific.
  • Sequence similarities
    Belongs to the POU transcription factor family. Class-2 subfamily.
    Contains 1 homeobox DNA-binding domain.
    Contains 1 POU-specific domain.
  • Post-translational
    modifications
    Phosphorylated by PRKDC.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ42836 antibody
    • NF A1 antibody
    • NF-A1 antibody
    • Oct 1 antibody
    • Oct 1B antibody
    • Oct-1 antibody
    • OCT1 antibody
    • Octamer binding protein 1 antibody
    • Octamer binding transcription factor 1 antibody
    • Octamer-binding protein 1 antibody
    • Octamer-binding transcription factor 1 antibody
    • OTF 1 antibody
    • OTF-1 antibody
    • OTF1 antibody
    • OTTHUMP00000032348 antibody
    • OTTHUMP00000032350 antibody
    • OTTHUMP00000032351 antibody
    • PO21 antibody
    • PO2F1 antibody
    • PO2F1_HUMAN antibody
    • POU class 2 homeobox 1 antibody
    • POU domain class 2 transcription factor 1 antibody
    • POU domain, class 2, transcription factor 1 antibody
    • POU2F1 antibody
    see all

Images

  • ab15112 staining Oct-1 in human tonsil by Immunohistochemistry (FFPE-sections).

  • All lanes : Anti-Oct-1 antibody (ab15112) at 1/100 dilution (in TBST + 5% milk for 16 hours at 4°C)

    All lanes : Whole cell lysate of human HEK293 cells

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : An HRP-conjugated Donkey anti-rabbit polyclonal at 1/1000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 89 kDa
    Observed band size: 89 kDa
    Additional bands at: 30 kDa, 45 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 20 seconds


    Blocking Step: 5% Milk for 1 hour at 22°C

References

This product has been referenced in:
  • Kitamura H  et al. Ubiquitin-Specific Protease 2 Modulates the Lipopolysaccharide-Elicited Expression of Proinflammatory Cytokines in Macrophage-like HL-60 Cells. Mediators Inflamm 2017:6909415 (2017). Read more (PubMed: 29138532) »
  • Oguri T  et al. Organic cation transporter 6 directly confers resistance to anticancer platinum drugs. Biomed Rep 5:639-643 (2016). WB ; Human . Read more (PubMed: 27882231) »
See all 7 Publications for this product

Customer reviews and Q&As

1-7 of 7 Abreviews or Q&A

Answer

Expiration date: 30-November-2012

I am very pleased to hear you would like to accept our offer and test ab15112, ab51363 and ab66132in Flow Cytometry. These codes will give you:3 free [PRIMARY ANTIBODY/PROTEIN] OR VALUE OFF OF ORDER before the expiration date. To redeem this offer, please submit an Abreview for [Flow Cytometry] and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Read More

Answer

Thank you for contacting us.

Could you let me know the sample type, species and application e.g. WB, IHC-P customer is interested in?

Please note the abreview discount code is valid only for untested species and applications. When sending the reply could you also send us customer details e.g. name, email address and University.

Please also note that the abreview testing discount is a 100% discount offer. This offer does not mean, customer would get free antibody in their initial purchase; they however have to buy the antibodies at full price and then after submitting the Abreview they will get free antibodies. Please check the link below for further information;

https://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Answer

Thank you for your interest.

We have some antibodies against Oct1 but currently none of them have been tested in Flow cytometry. These products have been characterized in IHC and WB.

https://www.abcam.com/ab15112: Anti-Oct1 antibody

https://www.abcam.com/ab66132: Anti-Oct1 antibody

https://www.abcam.com/ab51363: Anti-Oct1 antibody [POU5I097]

In case your customer wish to test any of these in Flow, I could offer a discount code for testing.

I would advise you to visit the following site where the Terms and Conditions of our testing initiative can be found at: https://www.abcam.com/collaborationdiscount.

If you need any further assistance in the future, please do not hesitate to contact me.

Read More

Answer

Thanks for your email. ab4587 does not have any required MSDS information, so a link will not be present on this datasheet. For example, ab7254 does have a link to MSDS information. I hope this helps. Please contact me again if you have any further questions. 

Read More

Answer

Thanks for your enquiry. If required, MSDS information is located via one or more 'MSDS' links near the top left corner of the online datasheet. This link is just below the 'Datasheet PDF' link present on all datasheets. I hope this is helpful. Please contact me again if you have any further questions.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (human HEK 293 cells)
Loading amount
20 µg
Specification
human HEK 293 cells
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Sep 16 2011

Answer

I received confirmation from the laboratory that tested this antibody that it gave a It 80kDa band on Jurkat lysate, however Raji lysate it gave a weak 80kDa band and a strong 32kDa band, which might be a truncated form. I would recommend to run the Jurkat lysate control along your samples to see what band size you see. Please do not hesitate to contact us if you require further assistance, we will be happy to look at your protocol in detail to see if we can provide some technical help,

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up