Product nameAnti-Oct-1 antibody [POU5I097]
See all Oct-1 primary antibodies
DescriptionMouse monoclonal [POU5I097] to Oct-1
Tested applicationsSuitable for: Flow Cyt, Dot blot, WB, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human Oct-1.
Database link: P14859
- HeLa cells, HEK293 whole cell lysate
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, 99% PBS
Concentration information loading...
PurityProtein G purified
Purification notesFiltered through a 0.22 µm membrane.
Our Abpromise guarantee covers the use of ab51363 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|Dot blot||Use at an assay dependent concentration.|
|WB||1/50. Detects a band of approximately 89 kDa (predicted molecular weight: 89 kDa).|
FunctionTranscription factor that binds to the octamer motif (5'-ATTTGCAT-3') and activates the promoters of the genes for some small nuclear RNAs (snRNA) and of genes such as those for histone H2B and immunoglobulins. Modulates transcription transactivation by NR3C1, AR and PGR (By similarity). In case of human herpes simplex virus (HSV) infection, POU2F1 forms a multiprotein-DNA complex with the viral transactivator protein VP16 and HCFC1 thereby enabling the transcription of the viral immediate early genes.
Tissue specificityUbiquitous. Isoform 2 is lymphocyte-specific.
Sequence similaritiesBelongs to the POU transcription factor family. Class-2 subfamily.
Contains 1 homeobox DNA-binding domain.
Contains 1 POU-specific domain.
modificationsPhosphorylated by PRKDC.
- Information by UniProt
- FLJ42836 antibody
- NF A1 antibody
- NF-A1 antibody
Ab51363 at 1:10 dilution staining HeLa cells; visualised with AlexaFluor®488 Goat Anti-mouse IgG at 1:200 dilution.
Anti-Oct-1 antibody [POU5I097] (ab51363) at 1/50 dilution + HEK293 whole cell lysate at 50 µg
Anti mouse IgG antibody at 1/2500 dilution
Predicted band size: 89 kDa
Observed band size: 89 kDa
Overlay histogram showing Jurkat cells stained with ab51363 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab51363, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1:500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
This product has been referenced in:
- Kang Z et al. Bacillus anthracis lethal toxin represses MMTV promoter activity through transcription factors. J Mol Biol 389:595-605 (2009). Read more (PubMed: 19389405) »
- Thum T & Borlak J Lox-1 receptor blockade abrogates OXLDL induced oxidative DNA damage and prevents activation of the transcriptional repressor OCT-1 in human coronary arterial endothelium. J Biol Chem : (2008). ICC/IF ; Human . Read more (PubMed: 18390905) »