Overview

  • Product name
    Anti-Oct-1 antibody [POU5I097]
    See all Oct-1 primary antibodies
  • Description
    Mouse monoclonal [POU5I097] to Oct-1
  • Host species
    Mouse
  • Tested applications
    Suitable for: Flow Cyt, Dot blot, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human Oct-1.
    Database link: P14859

  • Positive control
    • HeLa cells, HEK293 whole cell lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.40
    Preservative: 0.05% Sodium azide
    Constituents: 1% BSA, 99% PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    Filtered through a 0.22 µm membrane.
  • Clonality
    Monoclonal
  • Clone number
    POU5I097
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab51363 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Dot blot Use at an assay dependent concentration.
WB 1/50. Detects a band of approximately 89 kDa (predicted molecular weight: 89 kDa).
ICC/IF 1/10.

Target

  • Function
    Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and activates the promoters of the genes for some small nuclear RNAs (snRNA) and of genes such as those for histone H2B and immunoglobulins. Modulates transcription transactivation by NR3C1, AR and PGR (By similarity). In case of human herpes simplex virus (HSV) infection, POU2F1 forms a multiprotein-DNA complex with the viral transactivator protein VP16 and HCFC1 thereby enabling the transcription of the viral immediate early genes.
  • Tissue specificity
    Ubiquitous. Isoform 2 is lymphocyte-specific.
  • Sequence similarities
    Belongs to the POU transcription factor family. Class-2 subfamily.
    Contains 1 homeobox DNA-binding domain.
    Contains 1 POU-specific domain.
  • Post-translational
    modifications
    Phosphorylated by PRKDC.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ42836 antibody
    • NF A1 antibody
    • NF-A1 antibody
    • Oct 1 antibody
    • Oct 1B antibody
    • Oct-1 antibody
    • OCT1 antibody
    • Octamer binding protein 1 antibody
    • Octamer binding transcription factor 1 antibody
    • Octamer-binding protein 1 antibody
    • Octamer-binding transcription factor 1 antibody
    • OTF 1 antibody
    • OTF-1 antibody
    • OTF1 antibody
    • OTTHUMP00000032348 antibody
    • OTTHUMP00000032350 antibody
    • OTTHUMP00000032351 antibody
    • PO21 antibody
    • PO2F1 antibody
    • PO2F1_HUMAN antibody
    • POU class 2 homeobox 1 antibody
    • POU domain class 2 transcription factor 1 antibody
    • POU domain, class 2, transcription factor 1 antibody
    • POU2F1 antibody
    see all

Images

  • Ab51363 at 1:10 dilution staining HeLa cells; visualised with AlexaFluor®488 Goat Anti-mouse IgG at 1:200 dilution.

  • Anti-Oct-1 antibody [POU5I097] (ab51363) at 1/50 dilution + HEK293 whole cell lysate at 50 µg

    Secondary
    Anti mouse IgG antibody at 1/2500 dilution

    Predicted band size: 89 kDa
    Observed band size: 89 kDa

  • Overlay histogram showing Jurkat cells stained with ab51363 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab51363, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1:500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

References

This product has been referenced in:
  • Kang Z  et al. Bacillus anthracis lethal toxin represses MMTV promoter activity through transcription factors. J Mol Biol 389:595-605 (2009). Read more (PubMed: 19389405) »
  • Thum T & Borlak J Lox-1 receptor blockade abrogates OXLDL induced oxidative DNA damage and prevents activation of the transcriptional repressor OCT-1 in human coronary arterial endothelium. J Biol Chem : (2008). ICC/IF ; Human . Read more (PubMed: 18390905) »
See all 2 Publications for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (hiPSC)
Permeabilization
Yes - 0.1% Triton-X
Specification
hiPSC
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Paraformaldehyde

Jason Halliwell

Verified customer

Submitted Feb 08 2017

Answer

Thank you for your reply.

In brief, the protocol was: Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with ab51363.

Protocol:

1) Fix cells with 2-4% PFA in PBS for 10 min at RT.
2) Wash cells twice with PBS.
3) Permeabilize cells with 0.1% Triton X-100 in PBS.
4) Wash cells three times with PBS.
5) Block with 10% normal serum.
6) Incubate cells with first antibody in 2% normal serum for 1 hr at RT.
7) Wash cells in BPS three times for 5 min.
8) Incubate cells with secondary antibody in 2% normal serum for 1 hr at RT.
9) Wash cells in PBS three times in 5 min.
10) Mount with mounting medium and seal with nail polish.

I hope this information helps. Please contact us with any other questions.

Read More

Answer

Thank you for your inquiry. I am sorry to hear that you are having trouble with this antibody.

We have not tested, nor do we guarantee, this antibody to work in mouse. We have only tested this antibody with human samples. It may be that this antibody is not suitable to detect the mouse protein, but we are unsure since we haven't tested this ourselves.

I hope this information is helpful. Please contact us with any other questions.

Read More

Answer

Expiration date: 30-November-2012

I am very pleased to hear you would like to accept our offer and test ab15112, ab51363 and ab66132in Flow Cytometry. These codes will give you:3 free [PRIMARY ANTIBODY/PROTEIN] OR VALUE OFF OF ORDER before the expiration date. To redeem this offer, please submit an Abreview for [Flow Cytometry] and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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Answer

Thank you for contacting us.

Could you let me know the sample type, species and application e.g. WB, IHC-P customer is interested in?

Please note the abreview discount code is valid only for untested species and applications. When sending the reply could you also send us customer details e.g. name, email address and University.

Please also note that the abreview testing discount is a 100% discount offer. This offer does not mean, customer would get free antibody in their initial purchase; they however have to buy the antibodies at full price and then after submitting the Abreview they will get free antibodies. Please check the link below for further information;

https://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Answer

Thank you for your interest.

We have some antibodies against Oct1 but currently none of them have been tested in Flow cytometry. These products have been characterized in IHC and WB.

https://www.abcam.com/ab15112: Anti-Oct1 antibody

https://www.abcam.com/ab66132: Anti-Oct1 antibody

https://www.abcam.com/ab51363: Anti-Oct1 antibody [POU5I097]

In case your customer wish to test any of these in Flow, I could offer a discount code for testing.

I would advise you to visit the following site where the Terms and Conditions of our testing initiative can be found at: https://www.abcam.com/collaborationdiscount.

If you need any further assistance in the future, please do not hesitate to contact me.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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