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Anti-Oct-1 antibody [POU5I097] (ab51363)

Reviews (1)Q&A (5)References (3)
Immunocytochemistry/ Immunofluorescence - Anti-Oct-1 antibody [POU5I097] (ab51363)
  • Western blot - Anti-Oct-1 antibody [POU5I097] (ab51363)
  • Flow Cytometry - Anti-Oct-1 antibody [POU5I097] (ab51363)

Key features and details

  • Mouse monoclonal [POU5I097] to Oct-1
  • Suitable for: Flow Cyt, WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-Oct-1 antibody [POU5I097]
    See all Oct-1 primary antibodies

  • Description

    Mouse monoclonal [POU5I097] to Oct-1

  • Host species

    Mouse

  • Tested applications

    Suitable for: Flow Cyt, WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment corresponding to Human Oct-1.
    Database link: P14859

  • Positive control

    • HeLa cells, HEK293 whole cell lysate

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.40
    Preservative: 0.05% Sodium azide
    Constituents: 1% BSA, PBS
  • Concentration information loading...

  • Purity

    Protein G purified

  • Purification notes

    Filtered through a 0.22 µm membrane.

  • Clonality

    Monoclonal

  • Clone number

    POU5I097

  • Isotype

    IgG1

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab51363 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 
WB
1/50. Detects a band of approximately 89 kDa (predicted molecular weight: 89 kDa).
ICC/IF (1)
1/10.
Notes
Flow Cyt
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 
WB
1/50. Detects a band of approximately 89 kDa (predicted molecular weight: 89 kDa).
ICC/IF
1/10.

Target

  • Function

    Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and activates the promoters of the genes for some small nuclear RNAs (snRNA) and of genes such as those for histone H2B and immunoglobulins. Modulates transcription transactivation by NR3C1, AR and PGR (By similarity). In case of human herpes simplex virus (HSV) infection, POU2F1 forms a multiprotein-DNA complex with the viral transactivator protein VP16 and HCFC1 thereby enabling the transcription of the viral immediate early genes.

  • Tissue specificity

    Ubiquitous. Isoform 2 is lymphocyte-specific.

  • Sequence similarities

    Belongs to the POU transcription factor family. Class-2 subfamily.
    Contains 1 homeobox DNA-binding domain.
    Contains 1 POU-specific domain.

  • Post-translational
    modifications

    Phosphorylated by PRKDC.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • FLJ42836 antibody
      • NF A1 antibody
      • NF-A1 antibody
      • Oct 1 antibody
      • Oct 1B antibody
      • Oct-1 antibody
      • OCT1 antibody
      • Octamer binding protein 1 antibody
      • Octamer binding transcription factor 1 antibody
      • Octamer-binding protein 1 antibody
      • Octamer-binding transcription factor 1 antibody
      • OTF 1 antibody
      • OTF-1 antibody
      • OTF1 antibody
      • OTTHUMP00000032348 antibody
      • OTTHUMP00000032350 antibody
      • OTTHUMP00000032351 antibody
      • PO21 antibody
      • PO2F1 antibody
      • PO2F1_HUMAN antibody
      • POU class 2 homeobox 1 antibody
      • POU domain class 2 transcription factor 1 antibody
      • POU domain, class 2, transcription factor 1 antibody
      • POU2F1 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-Oct-1 antibody [POU5I097] (ab51363)
      Immunocytochemistry/ Immunofluorescence - Anti-Oct-1 antibody [POU5I097] (ab51363)

      Ab51363 at 1:10 dilution staining HeLa cells; visualised with AlexaFluor®488 Goat Anti-mouse IgG at 1:200 dilution.

    • Western blot - Anti-Oct-1 antibody [POU5I097] (ab51363)
      Western blot - Anti-Oct-1 antibody [POU5I097] (ab51363)
      Anti-Oct-1 antibody [POU5I097] (ab51363) at 1/50 dilution + HEK293 whole cell lysate at 50 µg

      Secondary
      Anti mouse IgG antibody at 1/2500 dilution

      Predicted band size: 89 kDa
      Observed band size: 89 kDa

    • Flow Cytometry - Anti-Oct-1 antibody [POU5I097] (ab51363)
      Flow Cytometry - Anti-Oct-1 antibody [POU5I097] (ab51363)

      Overlay histogram showing Jurkat cells stained with ab51363 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab51363, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1:500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    References (3)

    Publishing research using ab51363? Please let us know so that we can cite the reference in this datasheet.

    ab51363 has been referenced in 3 publications.

    • He W  et al. DNA methylation integratedly modulates the expression of Pit-Oct-Unt transcription factors in esophageal squamous cell carcinoma. J Cancer 12:1634-1643 (2021). PubMed: 33613750
    • Kang Z  et al. Bacillus anthracis lethal toxin represses MMTV promoter activity through transcription factors. J Mol Biol 389:595-605 (2009). PubMed: 19389405
    • Thum T & Borlak J Lox-1 receptor blockade abrogates OXLDL induced oxidative DNA damage and prevents activation of the transcriptional repressor OCT-1 in human coronary arterial endothelium. J Biol Chem : (2008). ICC/IF ; Human . PubMed: 18390905

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-5 of 5 Q&A

    Question

    May I have questions aboutthis antibody application?
    What fixation condition doyou use inyour IF data sheet(human cells)?

    Read More
    Answer

    Thank you for your reply.

    In brief, the protocol was: Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with ab51363.

    Protocol:

    1) Fix cells with 2-4% PFA in PBS for 10 min at RT.
    2) Wash cells twice with PBS.
    3) Permeabilize cells with 0.1% Triton X-100 in PBS.
    4) Wash cells three times with PBS.
    5) Block with 10% normal serum.
    6) Incubate cells with first antibody in 2% normal serum for 1 hr at RT.
    7) Wash cells in BPS three times for 5 min.
    8) Incubate cells with secondary antibody in 2% normal serum for 1 hr at RT.
    9) Wash cells in PBS three times in 5 min.
    10) Mount with mounting medium and seal with nail polish.

    I hope this information helps. Please contact us with any other questions.

    Read More

    Question

    We are customs of your company and we use lots of antibodies from your company.We had joined your testing program thisJune.I bought anti-Oct1(ab51363) antibody last week. I didMEF cells immunofluorescence staining(IF) using this antibody according reference. However, It does't work for me. May I return it for another Oct-1 antibody?

    Read More
    Answer

    Thank you for your inquiry. I am sorry to hear that you are having trouble with this antibody.

    We have not tested, nor do we guarantee, this antibody to work in mouse. We have only tested this antibody with human samples. It may be that this antibody is not suitable to detect the mouse protein, but we are unsure since we haven't tested this ourselves.

    I hope this information is helpful. Please contact us with any other questions.

    Read More

    Question

    The customer would be trying to use the antibodies on NTERA-2 cells(as
    postive control) and also on human MSC-like cells (experimental) for
    FACS and IHC but not too sure for WB.

    Read More
    Answer

    Expiration date: 30-November-2012

    I am very pleased to hear you would like to accept our offer and test ab15112, ab51363 and ab66132in Flow Cytometry. These codes will give you:3 free [PRIMARY ANTIBODY/PROTEIN] OR VALUE OFF OF ORDER before the expiration date. To redeem this offer, please submit an Abreview for [Flow Cytometry] and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
    For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

    Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

    Read More

    Question

    we would like to have the discount code as the customer is interested in testing some of the antibodies:

    https://www.abcam.com/ab15112: Anti-Oct1 antibody

    https://www.abcam.com/ab66132: Anti-Oct1 antibody

    https://www.abcam.com/ab51363: Anti-Oct1 antibody [POU5I097]

    May I know how much discount we can give?

    Hope to hear from you soon!
    Many Thanks and Best Regards,

    Read More
    Answer

    Thank you for contacting us.

    Could you let me know the sample type, species and application e.g. WB, IHC-P customer is interested in?

    Please note the abreview discount code is valid only for untested species and applications. When sending the reply could you also send us customer details e.g. name, email address and University.

    Please also note that the abreview testing discount is a 100% discount offer. This offer does not mean, customer would get free antibody in their initial purchase; they however have to buy the antibodies at full price and then after submitting the Abreview they will get free antibodies. Please check the link below for further information;

    https://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Question

    Dear Sir / Madam,

    thank you for your email,

    just one last request, the customer was thinking of getting oct 1 antibody, anti-human, for the purpose of FACS, ICC and WB but I could only find:

    https://www.abcam.com/Oct1-antibody-ab15112.html

    which is meant for only EMSA, ICC-P and WB but not FACS.

    What would you advise?

    Hope to hear from you soon :)

    Many Thanks and Best Regards,

    Read More
    Answer

    Thank you for your interest.

    We have some antibodies against Oct1 but currently none of them have been tested in Flow cytometry. These products have been characterized in IHC and WB.

    https://www.abcam.com/ab15112: Anti-Oct1 antibody

    https://www.abcam.com/ab66132: Anti-Oct1 antibody

    https://www.abcam.com/ab51363: Anti-Oct1 antibody [POU5I097]

    In case your customer wish to test any of these in Flow, I could offer a discount code for testing.

    I would advise you to visit the following site where the Terms and Conditions of our testing initiative can be found at: https://www.abcam.com/collaborationdiscount.

    If you need any further assistance in the future, please do not hesitate to contact me.

    Read More

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